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High genetic variability of Schistosoma haematobium in Mali and Nigeria.

Ezeh C, Yin M, Li H, Zhang T, Xu B, Sacko M, Feng Z, Hu W - Korean J. Parasitol. (2015)

Bottom Line: We found that the average allele number of individuals from Mali was significantly higher than that from Nigeria.In addition, no significant difference in allelic composition was detected among the populations within Nigeria; however, the allelic composition was significantly different between Mali and Nigeria populations.This study demonstrated a high level of genetic variability of S. haematobium in the populations from Mali and Nigeria, the 2 major African endemic countries, suggesting that geographical population differentiation may occur in the regions.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Parasite and Vector Biology, Ministry of Public Health, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.

ABSTRACT
Schistosoma haematobium is one of the most prevalent parasitic flatworms, infecting over 112 million people in Africa. However, little is known about the genetic diversity of natural S. haematobium populations from the human host because of the inaccessible location of adult worms in the host. We used 4 microsatellite loci to genotype individually pooled S. haematobium eggs directly from each patient sampled at 4 endemic locations in Africa. We found that the average allele number of individuals from Mali was significantly higher than that from Nigeria. In addition, no significant difference in allelic composition was detected among the populations within Nigeria; however, the allelic composition was significantly different between Mali and Nigeria populations. This study demonstrated a high level of genetic variability of S. haematobium in the populations from Mali and Nigeria, the 2 major African endemic countries, suggesting that geographical population differentiation may occur in the regions.

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Location of S. haematobium samples collected.
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f1-kjp-53-1-129: Location of S. haematobium samples collected.

Mentions: By applying a set of microsatellite markers, we genotyped pooled eggs of S. haematobium sampled directly from patients’urine samples. The S. haematobium egg samples were collected from 3 locations in Nigeria (Ebonyi, Bayelsa, and Ogun) and 1 location in Mali (Bamako) (Fig. 1). The egg samples were obtained by filtering single urine samples of infected primary school students, during May to November 2011. In total, 22 patients from Nigeria and 27 patients from Mali were enrolled for this study; all the patients were confirmed by the presence of S. haematobium eggs in the urine. The urine samples were then concentrated, and 5 eggs from each patient sample were fixed on Whatman FTA cards. The egg samples were delivered to the National Institute of Parasitic Diseases (NIPD), Chinese Center for Disease Control and Prevention (China CDC) for the study. The study protocol was approved by the Institutional Ethics Committee of NIPD, China CDC. The investigation aim, potential risks, and the benefits were explained to the participants, and the informed consents were obtained verbally. Treatment was followed to the subjects enrolled.


High genetic variability of Schistosoma haematobium in Mali and Nigeria.

Ezeh C, Yin M, Li H, Zhang T, Xu B, Sacko M, Feng Z, Hu W - Korean J. Parasitol. (2015)

Location of S. haematobium samples collected.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384788&req=5

f1-kjp-53-1-129: Location of S. haematobium samples collected.
Mentions: By applying a set of microsatellite markers, we genotyped pooled eggs of S. haematobium sampled directly from patients’urine samples. The S. haematobium egg samples were collected from 3 locations in Nigeria (Ebonyi, Bayelsa, and Ogun) and 1 location in Mali (Bamako) (Fig. 1). The egg samples were obtained by filtering single urine samples of infected primary school students, during May to November 2011. In total, 22 patients from Nigeria and 27 patients from Mali were enrolled for this study; all the patients were confirmed by the presence of S. haematobium eggs in the urine. The urine samples were then concentrated, and 5 eggs from each patient sample were fixed on Whatman FTA cards. The egg samples were delivered to the National Institute of Parasitic Diseases (NIPD), Chinese Center for Disease Control and Prevention (China CDC) for the study. The study protocol was approved by the Institutional Ethics Committee of NIPD, China CDC. The investigation aim, potential risks, and the benefits were explained to the participants, and the informed consents were obtained verbally. Treatment was followed to the subjects enrolled.

Bottom Line: We found that the average allele number of individuals from Mali was significantly higher than that from Nigeria.In addition, no significant difference in allelic composition was detected among the populations within Nigeria; however, the allelic composition was significantly different between Mali and Nigeria populations.This study demonstrated a high level of genetic variability of S. haematobium in the populations from Mali and Nigeria, the 2 major African endemic countries, suggesting that geographical population differentiation may occur in the regions.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Parasite and Vector Biology, Ministry of Public Health, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.

ABSTRACT
Schistosoma haematobium is one of the most prevalent parasitic flatworms, infecting over 112 million people in Africa. However, little is known about the genetic diversity of natural S. haematobium populations from the human host because of the inaccessible location of adult worms in the host. We used 4 microsatellite loci to genotype individually pooled S. haematobium eggs directly from each patient sampled at 4 endemic locations in Africa. We found that the average allele number of individuals from Mali was significantly higher than that from Nigeria. In addition, no significant difference in allelic composition was detected among the populations within Nigeria; however, the allelic composition was significantly different between Mali and Nigeria populations. This study demonstrated a high level of genetic variability of S. haematobium in the populations from Mali and Nigeria, the 2 major African endemic countries, suggesting that geographical population differentiation may occur in the regions.

Show MeSH