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Seropositivity and serointensity of Toxoplasma gondii antibodies and DNA among patients with schizophrenia.

Omar A, Bakar OC, Adam NF, Osman H, Osman A, Suleiman AH, Manaf MR, Selamat MI - Korean J. Parasitol. (2015)

Bottom Line: The serofrequency of Tg IgG antibodies (51.5%, 52/101) and DNA (32.67%, 33/101) among patients with schizophrenia was significantly higher than IgG (18.2%, 10/55) and DNA (3.64%, 2/55) of the controls (IgG, P=0.000, OD=4.8, CI=2.2-10.5; DNA, P=0.000, OD=12.9, CI=2.17-10.51).However, the Tg IgM antibody between patients with schizophrenia and controls was not significant (P>0.005).These findings have further demonstrated the strong association between the active Tg infection and schizophrenia.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine and Defence Health, National University of Defence, Malaysia.

ABSTRACT
The aim of this cross sectional case control study was to examine the serofrequency and serointensity of Toxoplasma gondii (Tg) IgG, IgM, and DNA among patients with schizophrenia. A total of 101 patients with schizophrenia and 55 healthy controls from Sungai Buloh Hospital, Selangor, Malaysia and University Malaya Medical Center (UMMC) were included in this study. The diagnosis of schizophrenia was made based on the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV). The presence of Tg infection was examined using both indirect (ELISA) and direct (quantitative real-time PCR) detection methods by measuring Tg IgG and IgM and DNA, respectively. The serofrequency of Tg IgG antibodies (51.5%, 52/101) and DNA (32.67%, 33/101) among patients with schizophrenia was significantly higher than IgG (18.2%, 10/55) and DNA (3.64%, 2/55) of the controls (IgG, P=0.000, OD=4.8, CI=2.2-10.5; DNA, P=0.000, OD=12.9, CI=2.17-10.51). However, the Tg IgM antibody between patients with schizophrenia and controls was not significant (P>0.005). There was no significant difference (P>0.005) in both serointensity of Tg IgG and DNA between patients with schizophrenia and controls. These findings have further demonstrated the strong association between the active Tg infection and schizophrenia.

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Quantity of T. gondii DNA in healthy controls by real-time PCR.
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f1-kjp-53-1-29: Quantity of T. gondii DNA in healthy controls by real-time PCR.

Mentions: Tg IgG antibody was positive in 52 (51.5%) and 10 (18.2%) of patients with schizophrenia and controls, respectively (P=0.0005, OD=4.78, 95% CI=2.17-10.51). Meanwhile, the IgM antibody was found in 4 (7.7%) of patients with schizophrenia and none (0%) in controls (OR=3.97, P=0.34, CI= 0.20-78.25). DNA for Tg was positive in 33 (32.67%) of patients with schizophrenia and 2 (3.64%) of controls (P= 0.0005, OD=12.86, CI=2.95-56.03). Table 3 shows the serointensity of Tg IgG, IgM, and DNA among patients and controls. The levels of Tg IgG antibody were not significantly different (P>0.005) in patients with schizophrenia (214.6±163.1 IU/ml) compared to controls (138.4±83.6 IU/ml, P=0.06). There was no significant difference between Tg DNA levels of patients with schizophrenia (0.09 ng/ml) vs controls (0.03± 0.65 ng/ml) (P=0.23). Figs. 1 and 2 show the data of qPCR in controls and patients with schizophrenia, respectively.


Seropositivity and serointensity of Toxoplasma gondii antibodies and DNA among patients with schizophrenia.

Omar A, Bakar OC, Adam NF, Osman H, Osman A, Suleiman AH, Manaf MR, Selamat MI - Korean J. Parasitol. (2015)

Quantity of T. gondii DNA in healthy controls by real-time PCR.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384787&req=5

f1-kjp-53-1-29: Quantity of T. gondii DNA in healthy controls by real-time PCR.
Mentions: Tg IgG antibody was positive in 52 (51.5%) and 10 (18.2%) of patients with schizophrenia and controls, respectively (P=0.0005, OD=4.78, 95% CI=2.17-10.51). Meanwhile, the IgM antibody was found in 4 (7.7%) of patients with schizophrenia and none (0%) in controls (OR=3.97, P=0.34, CI= 0.20-78.25). DNA for Tg was positive in 33 (32.67%) of patients with schizophrenia and 2 (3.64%) of controls (P= 0.0005, OD=12.86, CI=2.95-56.03). Table 3 shows the serointensity of Tg IgG, IgM, and DNA among patients and controls. The levels of Tg IgG antibody were not significantly different (P>0.005) in patients with schizophrenia (214.6±163.1 IU/ml) compared to controls (138.4±83.6 IU/ml, P=0.06). There was no significant difference between Tg DNA levels of patients with schizophrenia (0.09 ng/ml) vs controls (0.03± 0.65 ng/ml) (P=0.23). Figs. 1 and 2 show the data of qPCR in controls and patients with schizophrenia, respectively.

Bottom Line: The serofrequency of Tg IgG antibodies (51.5%, 52/101) and DNA (32.67%, 33/101) among patients with schizophrenia was significantly higher than IgG (18.2%, 10/55) and DNA (3.64%, 2/55) of the controls (IgG, P=0.000, OD=4.8, CI=2.2-10.5; DNA, P=0.000, OD=12.9, CI=2.17-10.51).However, the Tg IgM antibody between patients with schizophrenia and controls was not significant (P>0.005).These findings have further demonstrated the strong association between the active Tg infection and schizophrenia.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine and Defence Health, National University of Defence, Malaysia.

ABSTRACT
The aim of this cross sectional case control study was to examine the serofrequency and serointensity of Toxoplasma gondii (Tg) IgG, IgM, and DNA among patients with schizophrenia. A total of 101 patients with schizophrenia and 55 healthy controls from Sungai Buloh Hospital, Selangor, Malaysia and University Malaya Medical Center (UMMC) were included in this study. The diagnosis of schizophrenia was made based on the Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV). The presence of Tg infection was examined using both indirect (ELISA) and direct (quantitative real-time PCR) detection methods by measuring Tg IgG and IgM and DNA, respectively. The serofrequency of Tg IgG antibodies (51.5%, 52/101) and DNA (32.67%, 33/101) among patients with schizophrenia was significantly higher than IgG (18.2%, 10/55) and DNA (3.64%, 2/55) of the controls (IgG, P=0.000, OD=4.8, CI=2.2-10.5; DNA, P=0.000, OD=12.9, CI=2.17-10.51). However, the Tg IgM antibody between patients with schizophrenia and controls was not significant (P>0.005). There was no significant difference (P>0.005) in both serointensity of Tg IgG and DNA between patients with schizophrenia and controls. These findings have further demonstrated the strong association between the active Tg infection and schizophrenia.

Show MeSH
Related in: MedlinePlus