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Antileishmanial and cytotoxic effects of essential oil and methanolic extract of Myrtus communis L.

Mahmoudvand H, Ezzatkhah F, Sharififar F, Sharifi I, Dezaki ES - Korean J. Parasitol. (2015)

Bottom Line: The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model.Findings demonstrated that M. communis, particularly its essential oil, significantly (P<0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response.The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.

View Article: PubMed Central - PubMed

Affiliation: Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.

ABSTRACT
Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were α-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P<0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The IC50 values for essential oil and methanolic extract was 8.4 and 28.9 μg/ml against promastigotes, respectively. These values were 11.6 and 40.8 μg/ml against amastigote forms, respectively. Glucantime as control drug also revealed IC50 values of 88.3 and 44.6 μg/ml for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.

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The viability of Leishmania tropica promastigotes in the presence of various concentrations of the meglumine antimoniate (MA), essential oil, and methanolic extract of M. communis after 72 hr incubation. Data are expressed as the mean±SD (n=3).
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f1-kjp-53-1-21: The viability of Leishmania tropica promastigotes in the presence of various concentrations of the meglumine antimoniate (MA), essential oil, and methanolic extract of M. communis after 72 hr incubation. Data are expressed as the mean±SD (n=3).

Mentions: Anti-amastigote assay: The results showed that essential oil and methanolic extract of M. communis significantly (P<0.05) inhibited the growth rate of intramacrophage amastigotes as a dose-dependent manner (Fig. 1). Similar to anti-promastigote assay, the essential oil was more effective on the amastigote forms than the methanolic extract and MA once it exhibited the lower IC50 value for amastigote forms. The IC50 value for the essential oil (11.6 μg/ml) against promastigotes of L. tropica was significantly (P<0.05) lower than those of MA (44.6 μg/ml) and methanolic extract (40.8 μg/ml).


Antileishmanial and cytotoxic effects of essential oil and methanolic extract of Myrtus communis L.

Mahmoudvand H, Ezzatkhah F, Sharififar F, Sharifi I, Dezaki ES - Korean J. Parasitol. (2015)

The viability of Leishmania tropica promastigotes in the presence of various concentrations of the meglumine antimoniate (MA), essential oil, and methanolic extract of M. communis after 72 hr incubation. Data are expressed as the mean±SD (n=3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384785&req=5

f1-kjp-53-1-21: The viability of Leishmania tropica promastigotes in the presence of various concentrations of the meglumine antimoniate (MA), essential oil, and methanolic extract of M. communis after 72 hr incubation. Data are expressed as the mean±SD (n=3).
Mentions: Anti-amastigote assay: The results showed that essential oil and methanolic extract of M. communis significantly (P<0.05) inhibited the growth rate of intramacrophage amastigotes as a dose-dependent manner (Fig. 1). Similar to anti-promastigote assay, the essential oil was more effective on the amastigote forms than the methanolic extract and MA once it exhibited the lower IC50 value for amastigote forms. The IC50 value for the essential oil (11.6 μg/ml) against promastigotes of L. tropica was significantly (P<0.05) lower than those of MA (44.6 μg/ml) and methanolic extract (40.8 μg/ml).

Bottom Line: The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model.Findings demonstrated that M. communis, particularly its essential oil, significantly (P<0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response.The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.

View Article: PubMed Central - PubMed

Affiliation: Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.

ABSTRACT
Plants used for traditional medicine contain a wide range of substances that can be used to treat various diseases such as infectious diseases. The present study was designed to evaluate the antileishmanial effects of the essential oil and methanolic extract of Myrtus communis against Leishmania tropica on an in vitro model. Antileishmanial effects of essential oil and methanolic extract of M. communis on promastigote forms and their cytotoxic activities against J774 cells were evaluated using MTT assay for 72 hr. In addition, their leishmanicidal activity against amastigote forms was determined in a macrophage model, for 72 hr. Findings showed that the main components of essential oil were α-pinene (24.7%), 1,8-cineole (19.6%), and linalool (12.6%). Findings demonstrated that M. communis, particularly its essential oil, significantly (P<0.05) inhibited the growth rate of promastigote and amastigote forms of L. tropica based on a dose-dependent response. The IC50 values for essential oil and methanolic extract was 8.4 and 28.9 μg/ml against promastigotes, respectively. These values were 11.6 and 40.8 μg/ml against amastigote forms, respectively. Glucantime as control drug also revealed IC50 values of 88.3 and 44.6 μg/ml for promastigotes and amastigotes of L. tropica, respectively. The in vitro assay demonstrated no significant cytotoxicity in J774 cells. However, essential oil indicated a more cytotoxic effect as compared with the methanolic extract of M. communis. The findings of the present study demonstrated that M. communis might be a natural source for production of a new leishmanicidal agent.

Show MeSH
Related in: MedlinePlus