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Four additional cases of Diphyllobothrium nihonkaiense infection confirmed by analysis of COX1 gene in Korea.

Park SH, Jeon HK, Kim JB - Korean J. Parasitol. (2015)

Bottom Line: This paper reports confirmation of D. nihonkaiense infections in 4 additional human samples obtained between 1995 and 2014, which were analyzed at the Department of Parasitology, Hallym University College of Medicine, Korea.Analysis of the mitochondrial cytochrome c oxidase 1 (cox1) gene revealed a 98.5-99.5% similarity with a reference D. nihonkaiense sequence in GenBank.The present report adds 4 cases of D. nihonkaiense infections to the literature, indicating that the dominant diphyllobothriid tapeworm species in Korea is D. nihonkaiense but not D. latum.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Hallym University Chuncheon Sacred Heart Hospital, Chuncheon 200-704, Korea.

ABSTRACT
Most of the diphyllobothriid tapeworms isolated from human samples in the Republic of Korea (= Korea) have been identified as Diphyllobothrium nihonkaiense by genetic analysis. This paper reports confirmation of D. nihonkaiense infections in 4 additional human samples obtained between 1995 and 2014, which were analyzed at the Department of Parasitology, Hallym University College of Medicine, Korea. Analysis of the mitochondrial cytochrome c oxidase 1 (cox1) gene revealed a 98.5-99.5% similarity with a reference D. nihonkaiense sequence in GenBank. The present report adds 4 cases of D. nihonkaiense infections to the literature, indicating that the dominant diphyllobothriid tapeworm species in Korea is D. nihonkaiense but not D. latum.

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Aligned partial nucleotide sequences of a region of the mitochondrial cytochrome c oxidase subunit 1 gene of the DNA of adult Diphyllobothrium species. The sequences corresponded to 919-1,120 bp (total 203 bps) of the partial mitochondrial cox1 gene.
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f1-kjp-53-1-105: Aligned partial nucleotide sequences of a region of the mitochondrial cytochrome c oxidase subunit 1 gene of the DNA of adult Diphyllobothrium species. The sequences corresponded to 919-1,120 bp (total 203 bps) of the partial mitochondrial cox1 gene.

Mentions: The primers used were D1f (5´-TGTGTGGGGGCATCATATGT-3´) and D1r (5´-ATGATAAGGAACAGGAGCTCAGCATA-3´). The second PCR and sequencing primers used were D1f1 (5´-GTTTAGATGTAAAGACGGCTG-3´) and D1r1 (5´-GGTAAACCGCACACACCAAA-3´) and yielded a 203-bp product corresponding to the position 919-1,122 bp of the cox1 gene (Fig. 1).


Four additional cases of Diphyllobothrium nihonkaiense infection confirmed by analysis of COX1 gene in Korea.

Park SH, Jeon HK, Kim JB - Korean J. Parasitol. (2015)

Aligned partial nucleotide sequences of a region of the mitochondrial cytochrome c oxidase subunit 1 gene of the DNA of adult Diphyllobothrium species. The sequences corresponded to 919-1,120 bp (total 203 bps) of the partial mitochondrial cox1 gene.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384784&req=5

f1-kjp-53-1-105: Aligned partial nucleotide sequences of a region of the mitochondrial cytochrome c oxidase subunit 1 gene of the DNA of adult Diphyllobothrium species. The sequences corresponded to 919-1,120 bp (total 203 bps) of the partial mitochondrial cox1 gene.
Mentions: The primers used were D1f (5´-TGTGTGGGGGCATCATATGT-3´) and D1r (5´-ATGATAAGGAACAGGAGCTCAGCATA-3´). The second PCR and sequencing primers used were D1f1 (5´-GTTTAGATGTAAAGACGGCTG-3´) and D1r1 (5´-GGTAAACCGCACACACCAAA-3´) and yielded a 203-bp product corresponding to the position 919-1,122 bp of the cox1 gene (Fig. 1).

Bottom Line: This paper reports confirmation of D. nihonkaiense infections in 4 additional human samples obtained between 1995 and 2014, which were analyzed at the Department of Parasitology, Hallym University College of Medicine, Korea.Analysis of the mitochondrial cytochrome c oxidase 1 (cox1) gene revealed a 98.5-99.5% similarity with a reference D. nihonkaiense sequence in GenBank.The present report adds 4 cases of D. nihonkaiense infections to the literature, indicating that the dominant diphyllobothriid tapeworm species in Korea is D. nihonkaiense but not D. latum.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Hallym University Chuncheon Sacred Heart Hospital, Chuncheon 200-704, Korea.

ABSTRACT
Most of the diphyllobothriid tapeworms isolated from human samples in the Republic of Korea (= Korea) have been identified as Diphyllobothrium nihonkaiense by genetic analysis. This paper reports confirmation of D. nihonkaiense infections in 4 additional human samples obtained between 1995 and 2014, which were analyzed at the Department of Parasitology, Hallym University College of Medicine, Korea. Analysis of the mitochondrial cytochrome c oxidase 1 (cox1) gene revealed a 98.5-99.5% similarity with a reference D. nihonkaiense sequence in GenBank. The present report adds 4 cases of D. nihonkaiense infections to the literature, indicating that the dominant diphyllobothriid tapeworm species in Korea is D. nihonkaiense but not D. latum.

Show MeSH
Related in: MedlinePlus