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Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses.

Li CX, Shi M, Tian JH, Lin XD, Kang YJ, Chen LJ, Qin XC, Xu J, Holmes EC, Zhang YZ - Elife (2015)

Bottom Line: Although arthropods are important viral vectors, the biodiversity of arthropod viruses, as well as the role that arthropods have played in viral origins and evolution, is unclear.We similarly documented a remarkable diversity of genome structures in arthropod viruses, including a putative circular form, that sheds new light on the evolution of genome organization.Hence, arthropods are a major reservoir of viral genetic diversity and have likely been central to viral evolution.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

ABSTRACT
Although arthropods are important viral vectors, the biodiversity of arthropod viruses, as well as the role that arthropods have played in viral origins and evolution, is unclear. Through RNA sequencing of 70 arthropod species we discovered 112 novel viruses that appear to be ancestral to much of the documented genetic diversity of negative-sense RNA viruses, a number of which are also present as endogenous genomic copies. With this greatly enriched diversity we revealed that arthropods contain viruses that fall basal to major virus groups, including the vertebrate-specific arenaviruses, filoviruses, hantaviruses, influenza viruses, lyssaviruses, and paramyxoviruses. We similarly documented a remarkable diversity of genome structures in arthropod viruses, including a putative circular form, that sheds new light on the evolution of genome organization. Hence, arthropods are a major reservoir of viral genetic diversity and have likely been central to viral evolution.

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Related in: MedlinePlus

A fully labeled ML phylogeny for Orthomyxoviridae-likeviruses.The phylogeny is reconstructed using RdRp alignments. Statistical supportfrom the approximate likelihood-ratio test (aLRT) is shown on each nodeof the tree. The names of the viruses discovered in this study are shownin red. The names of reference sequences, which contain both the GenBankaccession number and the virus species name, are shown in black. Thenames of previously defined genera/families are shown to the right of thephylogenies.DOI:http://dx.doi.org/10.7554/eLife.05378.010
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fig3s1: A fully labeled ML phylogeny for Orthomyxoviridae-likeviruses.The phylogeny is reconstructed using RdRp alignments. Statistical supportfrom the approximate likelihood-ratio test (aLRT) is shown on each nodeof the tree. The names of the viruses discovered in this study are shownin red. The names of reference sequences, which contain both the GenBankaccession number and the virus species name, are shown in black. Thenames of previously defined genera/families are shown to the right of thephylogenies.DOI:http://dx.doi.org/10.7554/eLife.05378.010


Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses.

Li CX, Shi M, Tian JH, Lin XD, Kang YJ, Chen LJ, Qin XC, Xu J, Holmes EC, Zhang YZ - Elife (2015)

A fully labeled ML phylogeny for Orthomyxoviridae-likeviruses.The phylogeny is reconstructed using RdRp alignments. Statistical supportfrom the approximate likelihood-ratio test (aLRT) is shown on each nodeof the tree. The names of the viruses discovered in this study are shownin red. The names of reference sequences, which contain both the GenBankaccession number and the virus species name, are shown in black. Thenames of previously defined genera/families are shown to the right of thephylogenies.DOI:http://dx.doi.org/10.7554/eLife.05378.010
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384744&req=5

fig3s1: A fully labeled ML phylogeny for Orthomyxoviridae-likeviruses.The phylogeny is reconstructed using RdRp alignments. Statistical supportfrom the approximate likelihood-ratio test (aLRT) is shown on each nodeof the tree. The names of the viruses discovered in this study are shownin red. The names of reference sequences, which contain both the GenBankaccession number and the virus species name, are shown in black. Thenames of previously defined genera/families are shown to the right of thephylogenies.DOI:http://dx.doi.org/10.7554/eLife.05378.010
Bottom Line: Although arthropods are important viral vectors, the biodiversity of arthropod viruses, as well as the role that arthropods have played in viral origins and evolution, is unclear.We similarly documented a remarkable diversity of genome structures in arthropod viruses, including a putative circular form, that sheds new light on the evolution of genome organization.Hence, arthropods are a major reservoir of viral genetic diversity and have likely been central to viral evolution.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.

ABSTRACT
Although arthropods are important viral vectors, the biodiversity of arthropod viruses, as well as the role that arthropods have played in viral origins and evolution, is unclear. Through RNA sequencing of 70 arthropod species we discovered 112 novel viruses that appear to be ancestral to much of the documented genetic diversity of negative-sense RNA viruses, a number of which are also present as endogenous genomic copies. With this greatly enriched diversity we revealed that arthropods contain viruses that fall basal to major virus groups, including the vertebrate-specific arenaviruses, filoviruses, hantaviruses, influenza viruses, lyssaviruses, and paramyxoviruses. We similarly documented a remarkable diversity of genome structures in arthropod viruses, including a putative circular form, that sheds new light on the evolution of genome organization. Hence, arthropods are a major reservoir of viral genetic diversity and have likely been central to viral evolution.

Show MeSH
Related in: MedlinePlus