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A QTL for root growth angle on rice chromosome 7 is involved in the genetic pathway of DEEPER ROOTING 1.

Uga Y, Kitomi Y, Yamamoto E, Kanno N, Kawai S, Mizubayashi T, Fukuoka S - Rice (N Y) (2015)

Bottom Line: By crossing IR64 (which has a non-functional allele of DRO1) with Kinandang Patong (which has a functional allele of DRO1), we developed 26 chromosome segment substitution lines (CSSLs) that carried a particular chromosome segment from Kinandang Patong in the IR64 genetic background.Using these CSSLs, we found only one chromosomal region that was related to RGA: on chromosome 9, which includes DRO1.DRO3 may only affect RGA in plants with a functional DRO1 allele, suggesting that DRO3 is involved in the DRO1 genetic pathway.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602 Japan.

ABSTRACT

Background: Root growth angle (RGA) is an important trait that influences the ability of rice to avoid drought stress. DEEPER ROOTING 1 (DRO1), which is a major quantitative trait locus (QTL) for RGA, is responsible for the difference in RGA between the shallow-rooting cultivar IR64 and the deep-rooting cultivar Kinandang Patong. However, the RGA differences between these cultivars cannot be fully explained by DRO1. The objective of this study was to identify new QTLs for RGA explaining the difference in RGA between these cultivars.

Results: By crossing IR64 (which has a non-functional allele of DRO1) with Kinandang Patong (which has a functional allele of DRO1), we developed 26 chromosome segment substitution lines (CSSLs) that carried a particular chromosome segment from Kinandang Patong in the IR64 genetic background. Using these CSSLs, we found only one chromosomal region that was related to RGA: on chromosome 9, which includes DRO1. Using an F2 population derived from a cross between Kinandang Patong and the Dro1-NIL (near isogenic line), which had a functional DRO1 allele in the IR64 genetic background, we identified a new QTL for RGA (DRO3) on the long arm of chromosome 7.

Conclusions: DRO3 may only affect RGA in plants with a functional DRO1 allele, suggesting that DRO3 is involved in the DRO1 genetic pathway.

No MeSH data available.


Related in: MedlinePlus

Frequency distributions for the ratio of deep rooting and the root growth angle in the KD-F2 plants. Vertical and horizontal lines above the bars indicate the mean and SD in the parental lines.
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Fig3: Frequency distributions for the ratio of deep rooting and the root growth angle in the KD-F2 plants. Vertical and horizontal lines above the bars indicate the mean and SD in the parental lines.

Mentions: We developed an F2 population (KD-F2) derived from a cross between Kinandang Patong and Dro1-NIL because we did not find RGA QTLs in the IK-CSSLs having non-functional alleles of DRO1. When grown in the baskets, Dro1-NIL (RDR = 44.0%; RGA = 58.3°) had deeper roots than IR64 (RDR = 4.4%; RGA = 35.1°) but shallower roots than Kinandang Patong (RDR = 76.1%; RGA = 67.1°) (Figure 3). The RDRs in the KD-F2 plants showed transgressive segregation, with values ranging from 22.2% to 83.3%. The RGAs also showed transgressive segregation, with values ranging from 35.0° to 79.0°. Broad-sense heritabilities of RDR and RGA in KD-F2 plants were 68.7% and 57.1%, respectively.Figure 3


A QTL for root growth angle on rice chromosome 7 is involved in the genetic pathway of DEEPER ROOTING 1.

Uga Y, Kitomi Y, Yamamoto E, Kanno N, Kawai S, Mizubayashi T, Fukuoka S - Rice (N Y) (2015)

Frequency distributions for the ratio of deep rooting and the root growth angle in the KD-F2 plants. Vertical and horizontal lines above the bars indicate the mean and SD in the parental lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384719&req=5

Fig3: Frequency distributions for the ratio of deep rooting and the root growth angle in the KD-F2 plants. Vertical and horizontal lines above the bars indicate the mean and SD in the parental lines.
Mentions: We developed an F2 population (KD-F2) derived from a cross between Kinandang Patong and Dro1-NIL because we did not find RGA QTLs in the IK-CSSLs having non-functional alleles of DRO1. When grown in the baskets, Dro1-NIL (RDR = 44.0%; RGA = 58.3°) had deeper roots than IR64 (RDR = 4.4%; RGA = 35.1°) but shallower roots than Kinandang Patong (RDR = 76.1%; RGA = 67.1°) (Figure 3). The RDRs in the KD-F2 plants showed transgressive segregation, with values ranging from 22.2% to 83.3%. The RGAs also showed transgressive segregation, with values ranging from 35.0° to 79.0°. Broad-sense heritabilities of RDR and RGA in KD-F2 plants were 68.7% and 57.1%, respectively.Figure 3

Bottom Line: By crossing IR64 (which has a non-functional allele of DRO1) with Kinandang Patong (which has a functional allele of DRO1), we developed 26 chromosome segment substitution lines (CSSLs) that carried a particular chromosome segment from Kinandang Patong in the IR64 genetic background.Using these CSSLs, we found only one chromosomal region that was related to RGA: on chromosome 9, which includes DRO1.DRO3 may only affect RGA in plants with a functional DRO1 allele, suggesting that DRO3 is involved in the DRO1 genetic pathway.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602 Japan.

ABSTRACT

Background: Root growth angle (RGA) is an important trait that influences the ability of rice to avoid drought stress. DEEPER ROOTING 1 (DRO1), which is a major quantitative trait locus (QTL) for RGA, is responsible for the difference in RGA between the shallow-rooting cultivar IR64 and the deep-rooting cultivar Kinandang Patong. However, the RGA differences between these cultivars cannot be fully explained by DRO1. The objective of this study was to identify new QTLs for RGA explaining the difference in RGA between these cultivars.

Results: By crossing IR64 (which has a non-functional allele of DRO1) with Kinandang Patong (which has a functional allele of DRO1), we developed 26 chromosome segment substitution lines (CSSLs) that carried a particular chromosome segment from Kinandang Patong in the IR64 genetic background. Using these CSSLs, we found only one chromosomal region that was related to RGA: on chromosome 9, which includes DRO1. Using an F2 population derived from a cross between Kinandang Patong and the Dro1-NIL (near isogenic line), which had a functional DRO1 allele in the IR64 genetic background, we identified a new QTL for RGA (DRO3) on the long arm of chromosome 7.

Conclusions: DRO3 may only affect RGA in plants with a functional DRO1 allele, suggesting that DRO3 is involved in the DRO1 genetic pathway.

No MeSH data available.


Related in: MedlinePlus