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Anthocyanins From the Fruit of Vitis coignetiae Pulliat Potentiate the Cisplatin Activity by Inhibiting PI3K/Akt Signaling Pathways in Human Gastric Cancer Cells.

Lu JN, Lee WS, Nagappan A, Chang SH, Choi YH, Kim HJ, Kim GS, Ryu CH, Shin SC, Jung JM, Hong SC - J Cancer Prev (2015)

Bottom Line: In this study, in order to seeking an approach to increase the anti-cancer effects of CDDP with natural products.We found that AIMs enhanced anticancer effects of CDDP, which activity was additive but not synergistic.This study suggests that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat enhanced anti-cancer effects of CDDP by inhibiting Akt activity activated by CDDP.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Gyeongsang National University School of Medicine, Jinju.

ABSTRACT

Background: Cisplatin (cis-diaminedichloroplatinum, CDDP) is a widely used chemotherapeutic agent for the treatment of many cancers. However, initial resistance to CDDP is a serious problem in treating these cancers. Vitis coignetiae Pulliat (Meoru in Korea) have shown anti-nuclear factor kappa B and anti-epidermal growth factor receptor activities in cancer cells.

Methods: In this study, in order to seeking an approach to increase the anti-cancer effects of CDDP with natural products. Here, we investigated anthocyanins isolated from Vitis coignetiae Pulliat (anthocyanidins isolated from meoru, AIMs) can enhance anti-cancer effects of cisplatin (CDDP) in stomach cancer cells. The cell viability of SNU-1 and SNU-16 cells after treated with AIMs and CDDP were analyzed by MTT assay. The expressions of Akt and X-linked inhibitor of apoptosis protein (XIAP) proteins were examined by western blot in AIMs- and CDDP-treated cells.

Results: We found that AIMs enhanced anticancer effects of CDDP, which activity was additive but not synergistic. AIMs suppressed Akt activity of the cancer cells activated by CDDP. AIMs also suppressed in XIAP an anti-apoptotic protein.

Conclusions: This study suggests that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat enhanced anti-cancer effects of CDDP by inhibiting Akt activity activated by CDDP.

No MeSH data available.


Related in: MedlinePlus

The inhibitory effects of anthocyanins isolated from Vitis coignetiae Pulliat (anthocyanidins isolated from meoru, AIMs) and/or cis-diaminedichloroplatinum (CDDP) on SNU-1, and SNU-16 human gastric cancer cells. Cell proliferation was assessed by MTT assay. (A, B) SNU-1 cells were treated with indicated concentrations of AIMs for 24 hours. (C–F) SNU-16 cells were treated with indicated concentrations of AIMs for 24 hours. The data are shown as means ± SD of three independent experiments. aP < 0.05 versus the untreated group. bP < 0.01 versus the untreated group. cP < 0.05 versus the CDDP-treated group.
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f1-jcp-20-50: The inhibitory effects of anthocyanins isolated from Vitis coignetiae Pulliat (anthocyanidins isolated from meoru, AIMs) and/or cis-diaminedichloroplatinum (CDDP) on SNU-1, and SNU-16 human gastric cancer cells. Cell proliferation was assessed by MTT assay. (A, B) SNU-1 cells were treated with indicated concentrations of AIMs for 24 hours. (C–F) SNU-16 cells were treated with indicated concentrations of AIMs for 24 hours. The data are shown as means ± SD of three independent experiments. aP < 0.05 versus the untreated group. bP < 0.01 versus the untreated group. cP < 0.05 versus the CDDP-treated group.

Mentions: First, we assessed the effects of AIMs on the cell growth. MTT assay revealed that AIMs suppressed the proliferation of both the SNU-1 and SNU-16 cells in a dose-dependent manner (Fig. 1A and 1B). The degrees to inhibitory effects on the cell proliferation are similar in both cell lines. We previously found that SNU-1 cells are more sensitive to CDDP than SNU-16 cells at the high concentration that can induce apoptosis. At 100 μg/mL of AIMs, the difference in sensitivity to AIMs between SNU-1 and SNU-16 cells was not so much (Fig. 1C and 1D). Next, we checked the effects of CDDP and AIMs in the higher dose. As we previously showed higher CDDP (12 μg/mL) did not increased cell death in SNU-16 cells, but the increase in dose to 500 μg/mL of AIMs enhanced anticancer effects on SNU-16 cells, and also enhanced cytotoxicity of CDDP, but the enhanced cytotoxicity did not meet synergism (Fig. 1E and 1F) These results suggest that AIMs have anti-cancer cancer effects on SNU-16 cells which is resistant to CDDP.


Anthocyanins From the Fruit of Vitis coignetiae Pulliat Potentiate the Cisplatin Activity by Inhibiting PI3K/Akt Signaling Pathways in Human Gastric Cancer Cells.

Lu JN, Lee WS, Nagappan A, Chang SH, Choi YH, Kim HJ, Kim GS, Ryu CH, Shin SC, Jung JM, Hong SC - J Cancer Prev (2015)

The inhibitory effects of anthocyanins isolated from Vitis coignetiae Pulliat (anthocyanidins isolated from meoru, AIMs) and/or cis-diaminedichloroplatinum (CDDP) on SNU-1, and SNU-16 human gastric cancer cells. Cell proliferation was assessed by MTT assay. (A, B) SNU-1 cells were treated with indicated concentrations of AIMs for 24 hours. (C–F) SNU-16 cells were treated with indicated concentrations of AIMs for 24 hours. The data are shown as means ± SD of three independent experiments. aP < 0.05 versus the untreated group. bP < 0.01 versus the untreated group. cP < 0.05 versus the CDDP-treated group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384714&req=5

f1-jcp-20-50: The inhibitory effects of anthocyanins isolated from Vitis coignetiae Pulliat (anthocyanidins isolated from meoru, AIMs) and/or cis-diaminedichloroplatinum (CDDP) on SNU-1, and SNU-16 human gastric cancer cells. Cell proliferation was assessed by MTT assay. (A, B) SNU-1 cells were treated with indicated concentrations of AIMs for 24 hours. (C–F) SNU-16 cells were treated with indicated concentrations of AIMs for 24 hours. The data are shown as means ± SD of three independent experiments. aP < 0.05 versus the untreated group. bP < 0.01 versus the untreated group. cP < 0.05 versus the CDDP-treated group.
Mentions: First, we assessed the effects of AIMs on the cell growth. MTT assay revealed that AIMs suppressed the proliferation of both the SNU-1 and SNU-16 cells in a dose-dependent manner (Fig. 1A and 1B). The degrees to inhibitory effects on the cell proliferation are similar in both cell lines. We previously found that SNU-1 cells are more sensitive to CDDP than SNU-16 cells at the high concentration that can induce apoptosis. At 100 μg/mL of AIMs, the difference in sensitivity to AIMs between SNU-1 and SNU-16 cells was not so much (Fig. 1C and 1D). Next, we checked the effects of CDDP and AIMs in the higher dose. As we previously showed higher CDDP (12 μg/mL) did not increased cell death in SNU-16 cells, but the increase in dose to 500 μg/mL of AIMs enhanced anticancer effects on SNU-16 cells, and also enhanced cytotoxicity of CDDP, but the enhanced cytotoxicity did not meet synergism (Fig. 1E and 1F) These results suggest that AIMs have anti-cancer cancer effects on SNU-16 cells which is resistant to CDDP.

Bottom Line: In this study, in order to seeking an approach to increase the anti-cancer effects of CDDP with natural products.We found that AIMs enhanced anticancer effects of CDDP, which activity was additive but not synergistic.This study suggests that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat enhanced anti-cancer effects of CDDP by inhibiting Akt activity activated by CDDP.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Gyeongsang National University School of Medicine, Jinju.

ABSTRACT

Background: Cisplatin (cis-diaminedichloroplatinum, CDDP) is a widely used chemotherapeutic agent for the treatment of many cancers. However, initial resistance to CDDP is a serious problem in treating these cancers. Vitis coignetiae Pulliat (Meoru in Korea) have shown anti-nuclear factor kappa B and anti-epidermal growth factor receptor activities in cancer cells.

Methods: In this study, in order to seeking an approach to increase the anti-cancer effects of CDDP with natural products. Here, we investigated anthocyanins isolated from Vitis coignetiae Pulliat (anthocyanidins isolated from meoru, AIMs) can enhance anti-cancer effects of cisplatin (CDDP) in stomach cancer cells. The cell viability of SNU-1 and SNU-16 cells after treated with AIMs and CDDP were analyzed by MTT assay. The expressions of Akt and X-linked inhibitor of apoptosis protein (XIAP) proteins were examined by western blot in AIMs- and CDDP-treated cells.

Results: We found that AIMs enhanced anticancer effects of CDDP, which activity was additive but not synergistic. AIMs suppressed Akt activity of the cancer cells activated by CDDP. AIMs also suppressed in XIAP an anti-apoptotic protein.

Conclusions: This study suggests that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat enhanced anti-cancer effects of CDDP by inhibiting Akt activity activated by CDDP.

No MeSH data available.


Related in: MedlinePlus