Limits...
Carnosic Acid Inhibits Lipid Accumulation in 3T3-L1 Adipocytes Through Attenuation of Fatty Acid Desaturation.

Park MY, Sung MK - J Cancer Prev (2015)

Bottom Line: CA significantly decreased mRNA expression of peroxisome proliferator-activated receptor-γ, sterol regulatory element-binding protein 1, and CCAAT/enhancer binding protein-α in a dose-dependent manner.Moreover, it decreased the ratio of both C16:1/C16:0 and C18:1/C18:0, with reduced expression of stearoyl CoA desaturase 1 mRNA and protein.These results suggest that CA efficiently suppressed adipogenesis in 3T3-L1 adipocytes and its action, at least in part, is associated with the downregulation of adipogenesis-related genes and the fatty acid composition of TG accumulated in adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition Education, Graduate School of Education, Soonchunhyang University, Asan.

ABSTRACT

Background: Excess body fat accumulation contributes to the development of metabolic disorders that can cause adverse health effects. Carnosic acid (CA), a major bioactive component of rosemary (Rosemarinus officinalis), has been suggested to possess anti-adipogenic properties. The present study was conducted to elucidate the mechanism underlying the anti-adipogenic effects of CA.

Methods: 3T3-L1 pre-adipocytes were treated with CA (0.1, 1, and 10 μM) from day 0 to day 8 of differentiation. On day 8, biochemical markers of lipid accumulation and the degree of fatty acid desaturation were measured.

Results: Oil Red O staining results, triglyceride (TG) accumulation, and glycerol 3-phosphate dehydrogenase activity suggested that CA significantly inhibited lipid accumulation in 3T3-L1 adipocytes. CA significantly decreased mRNA expression of peroxisome proliferator-activated receptor-γ, sterol regulatory element-binding protein 1, and CCAAT/enhancer binding protein-α in a dose-dependent manner. Moreover, it decreased the ratio of both C16:1/C16:0 and C18:1/C18:0, with reduced expression of stearoyl CoA desaturase 1 mRNA and protein.

Conclusions: These results suggest that CA efficiently suppressed adipogenesis in 3T3-L1 adipocytes and its action, at least in part, is associated with the downregulation of adipogenesis-related genes and the fatty acid composition of TG accumulated in adipocytes.

No MeSH data available.


Related in: MedlinePlus

Effects of carnosic acid (CA) on adipocyte differentiation, triglyceride (TG) content, and glycerol 3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 cells. Preadipocytes were grown to confluency (day 0) and induced to differentiate with an optimized adipocyte differentiation medium in the presence of CA (0.1, 1, and 10 μM) throughout differentiation. (A) Morphological observation and Oil Red O staining of 3T3-L1 cells. After differentiation (day 8), the cells were fixed and stained with Oil Red O and photographed using a microscope (× 200). (B) Quantification of intracellular Oil Red O staining using spectrophotometry. (C) Quantification of TG content in 3T3-L1 cells. (D) GPDH activity in 3T3-L1 cells. Data are expressed as mean ± standard deviation (n = 4). a,b,cMeans with the different letters are significantly different from each other (P < 0.05) using Duncan’s multiple-range test.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4384713&req=5

f1-jcp-20-41: Effects of carnosic acid (CA) on adipocyte differentiation, triglyceride (TG) content, and glycerol 3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 cells. Preadipocytes were grown to confluency (day 0) and induced to differentiate with an optimized adipocyte differentiation medium in the presence of CA (0.1, 1, and 10 μM) throughout differentiation. (A) Morphological observation and Oil Red O staining of 3T3-L1 cells. After differentiation (day 8), the cells were fixed and stained with Oil Red O and photographed using a microscope (× 200). (B) Quantification of intracellular Oil Red O staining using spectrophotometry. (C) Quantification of TG content in 3T3-L1 cells. (D) GPDH activity in 3T3-L1 cells. Data are expressed as mean ± standard deviation (n = 4). a,b,cMeans with the different letters are significantly different from each other (P < 0.05) using Duncan’s multiple-range test.

Mentions: The effects of CA on the accumulation of intracellular lipid droplets in 3T3-L1 adipocytes are shown in Figure 1. Compared to the OD value of control cells, the OD values of Oil Red O in the cells treated with 1 μM and 10 μM CA significantly decreased by 19.1% and 33.4%, respectively, indicating reduced intracellular lipid accumulation (P < 0.05; Fig. 1B). Cellular TG was quantified, and adipocytes treated with CA at 1 μM and 10 μM showed reduced TG content by 15.5% and 39.8%, respectively, compared to that in the control cells (P < 0.05; Fig. 1C). The activity of cytosolic GPDH, a key regulatory enzyme involved in TG synthesis was also significantly decreased in CA-treated adipocytes compared to that in the control cells (P < 0.05; Fig. 1D).


Carnosic Acid Inhibits Lipid Accumulation in 3T3-L1 Adipocytes Through Attenuation of Fatty Acid Desaturation.

Park MY, Sung MK - J Cancer Prev (2015)

Effects of carnosic acid (CA) on adipocyte differentiation, triglyceride (TG) content, and glycerol 3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 cells. Preadipocytes were grown to confluency (day 0) and induced to differentiate with an optimized adipocyte differentiation medium in the presence of CA (0.1, 1, and 10 μM) throughout differentiation. (A) Morphological observation and Oil Red O staining of 3T3-L1 cells. After differentiation (day 8), the cells were fixed and stained with Oil Red O and photographed using a microscope (× 200). (B) Quantification of intracellular Oil Red O staining using spectrophotometry. (C) Quantification of TG content in 3T3-L1 cells. (D) GPDH activity in 3T3-L1 cells. Data are expressed as mean ± standard deviation (n = 4). a,b,cMeans with the different letters are significantly different from each other (P < 0.05) using Duncan’s multiple-range test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384713&req=5

f1-jcp-20-41: Effects of carnosic acid (CA) on adipocyte differentiation, triglyceride (TG) content, and glycerol 3-phosphate dehydrogenase (GPDH) activity in 3T3-L1 cells. Preadipocytes were grown to confluency (day 0) and induced to differentiate with an optimized adipocyte differentiation medium in the presence of CA (0.1, 1, and 10 μM) throughout differentiation. (A) Morphological observation and Oil Red O staining of 3T3-L1 cells. After differentiation (day 8), the cells were fixed and stained with Oil Red O and photographed using a microscope (× 200). (B) Quantification of intracellular Oil Red O staining using spectrophotometry. (C) Quantification of TG content in 3T3-L1 cells. (D) GPDH activity in 3T3-L1 cells. Data are expressed as mean ± standard deviation (n = 4). a,b,cMeans with the different letters are significantly different from each other (P < 0.05) using Duncan’s multiple-range test.
Mentions: The effects of CA on the accumulation of intracellular lipid droplets in 3T3-L1 adipocytes are shown in Figure 1. Compared to the OD value of control cells, the OD values of Oil Red O in the cells treated with 1 μM and 10 μM CA significantly decreased by 19.1% and 33.4%, respectively, indicating reduced intracellular lipid accumulation (P < 0.05; Fig. 1B). Cellular TG was quantified, and adipocytes treated with CA at 1 μM and 10 μM showed reduced TG content by 15.5% and 39.8%, respectively, compared to that in the control cells (P < 0.05; Fig. 1C). The activity of cytosolic GPDH, a key regulatory enzyme involved in TG synthesis was also significantly decreased in CA-treated adipocytes compared to that in the control cells (P < 0.05; Fig. 1D).

Bottom Line: CA significantly decreased mRNA expression of peroxisome proliferator-activated receptor-γ, sterol regulatory element-binding protein 1, and CCAAT/enhancer binding protein-α in a dose-dependent manner.Moreover, it decreased the ratio of both C16:1/C16:0 and C18:1/C18:0, with reduced expression of stearoyl CoA desaturase 1 mRNA and protein.These results suggest that CA efficiently suppressed adipogenesis in 3T3-L1 adipocytes and its action, at least in part, is associated with the downregulation of adipogenesis-related genes and the fatty acid composition of TG accumulated in adipocytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Food and Nutrition Education, Graduate School of Education, Soonchunhyang University, Asan.

ABSTRACT

Background: Excess body fat accumulation contributes to the development of metabolic disorders that can cause adverse health effects. Carnosic acid (CA), a major bioactive component of rosemary (Rosemarinus officinalis), has been suggested to possess anti-adipogenic properties. The present study was conducted to elucidate the mechanism underlying the anti-adipogenic effects of CA.

Methods: 3T3-L1 pre-adipocytes were treated with CA (0.1, 1, and 10 μM) from day 0 to day 8 of differentiation. On day 8, biochemical markers of lipid accumulation and the degree of fatty acid desaturation were measured.

Results: Oil Red O staining results, triglyceride (TG) accumulation, and glycerol 3-phosphate dehydrogenase activity suggested that CA significantly inhibited lipid accumulation in 3T3-L1 adipocytes. CA significantly decreased mRNA expression of peroxisome proliferator-activated receptor-γ, sterol regulatory element-binding protein 1, and CCAAT/enhancer binding protein-α in a dose-dependent manner. Moreover, it decreased the ratio of both C16:1/C16:0 and C18:1/C18:0, with reduced expression of stearoyl CoA desaturase 1 mRNA and protein.

Conclusions: These results suggest that CA efficiently suppressed adipogenesis in 3T3-L1 adipocytes and its action, at least in part, is associated with the downregulation of adipogenesis-related genes and the fatty acid composition of TG accumulated in adipocytes.

No MeSH data available.


Related in: MedlinePlus