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Auxin regulates SNARE-dependent vacuolar morphology restricting cell size.

Löfke C, Dünser K, Scheuring D, Kleine-Vehn J - Elife (2015)

Bottom Line: Here, we reveal that the phytohormone auxin impacts on the shape of the biggest plant organelle, the vacuole.Genetic and pharmacological interference with the auxin effect on vacuolar SNAREs interrelates with auxin-resistant vacuolar morphogenesis and cell size regulation.Vacuolar SNARE VTI11 is strictly required for auxin-reliant vacuolar morphogenesis and loss of function renders cells largely insensitive to auxin-dependent growth inhibition.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.

ABSTRACT
The control of cellular growth is central to multicellular patterning. In plants, the encapsulating cell wall literally binds neighbouring cells to each other and limits cellular sliding/migration. In contrast to its developmental importance, growth regulation is poorly understood in plants. Here, we reveal that the phytohormone auxin impacts on the shape of the biggest plant organelle, the vacuole. TIR1/AFBs-dependent auxin signalling posttranslationally controls the protein abundance of vacuolar SNARE components. Genetic and pharmacological interference with the auxin effect on vacuolar SNAREs interrelates with auxin-resistant vacuolar morphogenesis and cell size regulation. Vacuolar SNARE VTI11 is strictly required for auxin-reliant vacuolar morphogenesis and loss of function renders cells largely insensitive to auxin-dependent growth inhibition. Our data suggests that the adaptation of SNARE-dependent vacuolar morphogenesis allows auxin to limit cellular expansion, contributing to root organ growth rates.

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Auxin does not affect vacuolar morphology of epidermal root cells in the differentiation zone.DMSO (A and C) or NAA (B and D) (250 nM, 20 hr) -treated pUBQ10::VAMP711-YFP (orange) expressing seedlings imaged at the onset of root hair bulging (differentiation zone). Propidium-iodide (PI)-stained cell walls (green). (C and D) Overlay of YFP and PI. Scale bar: 50 µm.DOI:http://dx.doi.org/10.7554/eLife.05868.005
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fig1s2: Auxin does not affect vacuolar morphology of epidermal root cells in the differentiation zone.DMSO (A and C) or NAA (B and D) (250 nM, 20 hr) -treated pUBQ10::VAMP711-YFP (orange) expressing seedlings imaged at the onset of root hair bulging (differentiation zone). Propidium-iodide (PI)-stained cell walls (green). (C and D) Overlay of YFP and PI. Scale bar: 50 µm.DOI:http://dx.doi.org/10.7554/eLife.05868.005

Mentions: Our data shows that the auxin effect on vacuolar morphology correlates with its negative effect on late meristematic epidermal cell size. Auxin treatments manifestly did not reverse vacuolar morphology of fully elongated epidermal root cells in the differentiation zone (Figure 1—figure supplement 2). This finding suggests that auxin mainly shapes vacuoles in growth competent cells.


Auxin regulates SNARE-dependent vacuolar morphology restricting cell size.

Löfke C, Dünser K, Scheuring D, Kleine-Vehn J - Elife (2015)

Auxin does not affect vacuolar morphology of epidermal root cells in the differentiation zone.DMSO (A and C) or NAA (B and D) (250 nM, 20 hr) -treated pUBQ10::VAMP711-YFP (orange) expressing seedlings imaged at the onset of root hair bulging (differentiation zone). Propidium-iodide (PI)-stained cell walls (green). (C and D) Overlay of YFP and PI. Scale bar: 50 µm.DOI:http://dx.doi.org/10.7554/eLife.05868.005
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384535&req=5

fig1s2: Auxin does not affect vacuolar morphology of epidermal root cells in the differentiation zone.DMSO (A and C) or NAA (B and D) (250 nM, 20 hr) -treated pUBQ10::VAMP711-YFP (orange) expressing seedlings imaged at the onset of root hair bulging (differentiation zone). Propidium-iodide (PI)-stained cell walls (green). (C and D) Overlay of YFP and PI. Scale bar: 50 µm.DOI:http://dx.doi.org/10.7554/eLife.05868.005
Mentions: Our data shows that the auxin effect on vacuolar morphology correlates with its negative effect on late meristematic epidermal cell size. Auxin treatments manifestly did not reverse vacuolar morphology of fully elongated epidermal root cells in the differentiation zone (Figure 1—figure supplement 2). This finding suggests that auxin mainly shapes vacuoles in growth competent cells.

Bottom Line: Here, we reveal that the phytohormone auxin impacts on the shape of the biggest plant organelle, the vacuole.Genetic and pharmacological interference with the auxin effect on vacuolar SNAREs interrelates with auxin-resistant vacuolar morphogenesis and cell size regulation.Vacuolar SNARE VTI11 is strictly required for auxin-reliant vacuolar morphogenesis and loss of function renders cells largely insensitive to auxin-dependent growth inhibition.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria.

ABSTRACT
The control of cellular growth is central to multicellular patterning. In plants, the encapsulating cell wall literally binds neighbouring cells to each other and limits cellular sliding/migration. In contrast to its developmental importance, growth regulation is poorly understood in plants. Here, we reveal that the phytohormone auxin impacts on the shape of the biggest plant organelle, the vacuole. TIR1/AFBs-dependent auxin signalling posttranslationally controls the protein abundance of vacuolar SNARE components. Genetic and pharmacological interference with the auxin effect on vacuolar SNAREs interrelates with auxin-resistant vacuolar morphogenesis and cell size regulation. Vacuolar SNARE VTI11 is strictly required for auxin-reliant vacuolar morphogenesis and loss of function renders cells largely insensitive to auxin-dependent growth inhibition. Our data suggests that the adaptation of SNARE-dependent vacuolar morphogenesis allows auxin to limit cellular expansion, contributing to root organ growth rates.

Show MeSH
Related in: MedlinePlus