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Montelukast inhibits pentylenetetrazol-induced seizures in rats.

Cevik B, Solmaz V, Aksoy D, Erbas O - Med. Sci. Monit. (2015)

Bottom Line: Animals treated with 50 or 100 mg/kg montelukast had significantly lower RCS and significantly increased FMJ onset time compared to the saline-treated animals.Moreover, groups given 25, 50, or 100 mg/kg montelukast had significantly lower MDA and higher SOD levels compared to the saline-treated group.The differences were more pronounced in the 100 mg/kg montelukast-pretreated group (p<0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.

ABSTRACT

Background: Montelukast is an antiinflammatory drug with an antioxidant property. In this study, we aimed to reveal whether montelukast has a preventive effect against seizures and post-seizure oxidative stress in pentylenetetrazol (PTZ)-induced seizures in rats.

Material and methods: Of the 48 male Sprague-Dawley rats used in the study, 24 were assigned to EEG recordings (group A) and 24 were assigned to behavioral studies (group B). In group A, the electrodes were implanted on dura over the left frontal cortex for EEG recording. After 10 days, in group A, i.p. saline, 25, 50, or 100 mg/kg montelukast+35 mg/kg PTZ was administered to the rats. EEG was recorded and spike percentage was evaluated. In group B, i.p. saline, 25, 50, or 100 mg/kg montelukast+70 mg/kg PTZ was administered to the rats. Racine's Convulsion Scale (RCS) and onset times of first myoclonic jerk (FMJ) was used to evaluate the seizures. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined in the brain tissue of animals.

Results: Animals treated with 50 or 100 mg/kg montelukast had significantly lower RCS and significantly increased FMJ onset time compared to the saline-treated animals. Moreover, groups given 25, 50, or 100 mg/kg montelukast had significantly lower MDA and higher SOD levels compared to the saline-treated group. The differences were more pronounced in the 100 mg/kg montelukast-pretreated group (p<0.001).

Conclusions: Montelukast showed anticonvulsant action and led to amelioration of oxidative stress markers in PTZ-induced seizures in rats.

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Related in: MedlinePlus

EEG recording (A): PTZ (35 mg/kg) and saline, (B): PTZ (35 mg/kg) and 25 mg/kg montelukast group; (C): PTZ (35 mg/kg) and 50 mg/kg montelukast group; (D): PTZ (35 mg/kg) and 100 mg/kg montelukast group.
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f1-medscimonit-21-869: EEG recording (A): PTZ (35 mg/kg) and saline, (B): PTZ (35 mg/kg) and 25 mg/kg montelukast group; (C): PTZ (35 mg/kg) and 50 mg/kg montelukast group; (D): PTZ (35 mg/kg) and 100 mg/kg montelukast group.

Mentions: Group A1 was treated with i.p. saline, while Groups A2, A3, and A4 were treated with 25, 50, and 100 mg/kg i.p. montelukast (Singulair, Merck Sharp & Dohme BV Waarderweg 39, Haarlem – The Netherlands), respectively. The drugs were administered 30 min prior to the PTZ injection (35 mg/kg, i.p.) (Sigma-Aldrich). All these 4 groups received 35 mg/kg PTZ i.p. and EEG was recorded. EEG recordings were taken in conscious rats in a special container 5 min after PTZ administration. The EEG recording was made during a 60-min period (Figure 1) [13]. The EEG signals were amplified 10 000 times and filtered with a range of 1–60 Hz. EEG records were taken by using the Biopac MP 150 amplifier system and 2 clinical neurophysiologists (BC and DA) scored the EEG data for spike percentage.


Montelukast inhibits pentylenetetrazol-induced seizures in rats.

Cevik B, Solmaz V, Aksoy D, Erbas O - Med. Sci. Monit. (2015)

EEG recording (A): PTZ (35 mg/kg) and saline, (B): PTZ (35 mg/kg) and 25 mg/kg montelukast group; (C): PTZ (35 mg/kg) and 50 mg/kg montelukast group; (D): PTZ (35 mg/kg) and 100 mg/kg montelukast group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4384514&req=5

f1-medscimonit-21-869: EEG recording (A): PTZ (35 mg/kg) and saline, (B): PTZ (35 mg/kg) and 25 mg/kg montelukast group; (C): PTZ (35 mg/kg) and 50 mg/kg montelukast group; (D): PTZ (35 mg/kg) and 100 mg/kg montelukast group.
Mentions: Group A1 was treated with i.p. saline, while Groups A2, A3, and A4 were treated with 25, 50, and 100 mg/kg i.p. montelukast (Singulair, Merck Sharp & Dohme BV Waarderweg 39, Haarlem – The Netherlands), respectively. The drugs were administered 30 min prior to the PTZ injection (35 mg/kg, i.p.) (Sigma-Aldrich). All these 4 groups received 35 mg/kg PTZ i.p. and EEG was recorded. EEG recordings were taken in conscious rats in a special container 5 min after PTZ administration. The EEG recording was made during a 60-min period (Figure 1) [13]. The EEG signals were amplified 10 000 times and filtered with a range of 1–60 Hz. EEG records were taken by using the Biopac MP 150 amplifier system and 2 clinical neurophysiologists (BC and DA) scored the EEG data for spike percentage.

Bottom Line: Animals treated with 50 or 100 mg/kg montelukast had significantly lower RCS and significantly increased FMJ onset time compared to the saline-treated animals.Moreover, groups given 25, 50, or 100 mg/kg montelukast had significantly lower MDA and higher SOD levels compared to the saline-treated group.The differences were more pronounced in the 100 mg/kg montelukast-pretreated group (p<0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.

ABSTRACT

Background: Montelukast is an antiinflammatory drug with an antioxidant property. In this study, we aimed to reveal whether montelukast has a preventive effect against seizures and post-seizure oxidative stress in pentylenetetrazol (PTZ)-induced seizures in rats.

Material and methods: Of the 48 male Sprague-Dawley rats used in the study, 24 were assigned to EEG recordings (group A) and 24 were assigned to behavioral studies (group B). In group A, the electrodes were implanted on dura over the left frontal cortex for EEG recording. After 10 days, in group A, i.p. saline, 25, 50, or 100 mg/kg montelukast+35 mg/kg PTZ was administered to the rats. EEG was recorded and spike percentage was evaluated. In group B, i.p. saline, 25, 50, or 100 mg/kg montelukast+70 mg/kg PTZ was administered to the rats. Racine's Convulsion Scale (RCS) and onset times of first myoclonic jerk (FMJ) was used to evaluate the seizures. Malondialdehyde (MDA) and superoxide dismutase (SOD) levels were determined in the brain tissue of animals.

Results: Animals treated with 50 or 100 mg/kg montelukast had significantly lower RCS and significantly increased FMJ onset time compared to the saline-treated animals. Moreover, groups given 25, 50, or 100 mg/kg montelukast had significantly lower MDA and higher SOD levels compared to the saline-treated group. The differences were more pronounced in the 100 mg/kg montelukast-pretreated group (p<0.001).

Conclusions: Montelukast showed anticonvulsant action and led to amelioration of oxidative stress markers in PTZ-induced seizures in rats.

Show MeSH
Related in: MedlinePlus