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Characteristics of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa in Korea.

Hong JS, Kim JO, Lee H, Bae IK, Jeong SH, Lee K - Infect Chemother (2015)

Bottom Line: All MBL-producing isolates showed multi-drug resistant phenotype, and a majority (n = 19) of the isolates were identified as sequence type 235 (ST235).The remaining isolates (n = 2) were identified as ST309 and ST463.P. aeruginosa ST235 might play an important role in dissemination of MBL genes in Korea.

View Article: PubMed Central - PubMed

Affiliation: Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Korea.

ABSTRACT

Background: The aim of this study was to investigate the molecular epidemiological characteristics of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa clinical isolates in Korea.

Materials and methods: Three hundred and twenty nine P. aeruginosa clinical isolates were collected from 23 general hospitals in Korea from March to June 2014. Species were identified by matrix-assited laser desorption/ionization-time of flight and 16S rRNA sequencing. Antimicrobial susceptibility was determined by disk diffusion methods. Further, minimum inhibitory concentrations of carbapenems were determined by Etest. Polymerase chain reaction and sequencing were performed to identify genes encoding MBLs. Multi-locus sequence typing and pulsed-field gel electrophoresis were performed to determine epidemiological characteristics of MBL-producing P. aeruginosa isolates.

Results: Of the 329 isolates, 229 (69.6%) were susceptible to the carbapenems tested, including imipenem and meropenem; while 100 (30.4%) were non-susceptible to more than one of the carbapenems. Genes encoding imipenemase-6 (IMP-6) and Verona imipenemase-2 (VIM-2) MBLs were identified in 21 (6.4%) isolates (n = 17 and 4, respectively). All MBL-producing isolates showed multi-drug resistant phenotype, and a majority (n = 19) of the isolates were identified as sequence type 235 (ST235). The remaining isolates (n = 2) were identified as ST309 and ST463.

Conclusion: P. aeruginosa ST235 might play an important role in dissemination of MBL genes in Korea.

No MeSH data available.


Related in: MedlinePlus

Map showing the locations of the participating hospitals in this study.MBL, metallo-β-lactamase; IMP, imipenemase; VIM, Verona imipenemase.
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Figure 1: Map showing the locations of the participating hospitals in this study.MBL, metallo-β-lactamase; IMP, imipenemase; VIM, Verona imipenemase.

Mentions: Non-duplicate P. aeruginosa clinical isolates (n = 329) were collected from 23 hospitals across 15 cities in Korea (Fig. 1) from March to June, 2014. The isolates were recovered from respiratory specimens (n = 120), urine (n = 96), pus (n = 78), blood (n = 24), and others (n = 11) (Table 1). Species identification was performed using the Bruker MALDI Biotyper (Bruker, Billerica, MA, USA) and 16S rRNA gene sequencing.


Characteristics of Metallo-β-Lactamase-Producing Pseudomonas aeruginosa in Korea.

Hong JS, Kim JO, Lee H, Bae IK, Jeong SH, Lee K - Infect Chemother (2015)

Map showing the locations of the participating hospitals in this study.MBL, metallo-β-lactamase; IMP, imipenemase; VIM, Verona imipenemase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384452&req=5

Figure 1: Map showing the locations of the participating hospitals in this study.MBL, metallo-β-lactamase; IMP, imipenemase; VIM, Verona imipenemase.
Mentions: Non-duplicate P. aeruginosa clinical isolates (n = 329) were collected from 23 hospitals across 15 cities in Korea (Fig. 1) from March to June, 2014. The isolates were recovered from respiratory specimens (n = 120), urine (n = 96), pus (n = 78), blood (n = 24), and others (n = 11) (Table 1). Species identification was performed using the Bruker MALDI Biotyper (Bruker, Billerica, MA, USA) and 16S rRNA gene sequencing.

Bottom Line: All MBL-producing isolates showed multi-drug resistant phenotype, and a majority (n = 19) of the isolates were identified as sequence type 235 (ST235).The remaining isolates (n = 2) were identified as ST309 and ST463.P. aeruginosa ST235 might play an important role in dissemination of MBL genes in Korea.

View Article: PubMed Central - PubMed

Affiliation: Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Korea.

ABSTRACT

Background: The aim of this study was to investigate the molecular epidemiological characteristics of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa clinical isolates in Korea.

Materials and methods: Three hundred and twenty nine P. aeruginosa clinical isolates were collected from 23 general hospitals in Korea from March to June 2014. Species were identified by matrix-assited laser desorption/ionization-time of flight and 16S rRNA sequencing. Antimicrobial susceptibility was determined by disk diffusion methods. Further, minimum inhibitory concentrations of carbapenems were determined by Etest. Polymerase chain reaction and sequencing were performed to identify genes encoding MBLs. Multi-locus sequence typing and pulsed-field gel electrophoresis were performed to determine epidemiological characteristics of MBL-producing P. aeruginosa isolates.

Results: Of the 329 isolates, 229 (69.6%) were susceptible to the carbapenems tested, including imipenem and meropenem; while 100 (30.4%) were non-susceptible to more than one of the carbapenems. Genes encoding imipenemase-6 (IMP-6) and Verona imipenemase-2 (VIM-2) MBLs were identified in 21 (6.4%) isolates (n = 17 and 4, respectively). All MBL-producing isolates showed multi-drug resistant phenotype, and a majority (n = 19) of the isolates were identified as sequence type 235 (ST235). The remaining isolates (n = 2) were identified as ST309 and ST463.

Conclusion: P. aeruginosa ST235 might play an important role in dissemination of MBL genes in Korea.

No MeSH data available.


Related in: MedlinePlus