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The time-course and RNA interference of TNF-α, IL-6, and IL-1β expression on neuropathic pain induced by L5 spinal nerve transection in rats.

Choi BM, Lee SH, An SM, Park do Y, Lee GW, Noh GJ - Korean J Anesthesiol (2015)

Bottom Line: Behavioral tests and levels of the cytokine mRNAs and proteins as well as glial cell activity were following the L5 SNT.In the behavioral tests, allodynia and hyperalgesia were significantly lower in the COCK group from 2 d after SNT (P < 0.05).RNA interference may be a method of reducing the development of mechanical allodynia and hyperalgesia in response to nerve injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

ABSTRACT

Background: The objective of this study was to investigate the time-course of the expression of TNF-α, IL-6, and IL-1β after L5 spinal nerve transection (SNT), and to determine the effect of small interfering RNA (siRNA) targeting these cytokines on neuropathic pain.

Methods: Rats received control siRNA (CON group, n = 80) or a cocktail of siRNAs targeting these cytokines (COCK group, n = 70). The siRNAs were given via intrathecal catheter 1 d prior to SNT, on the operation day, and 1, 2 and 3 d postoperatively. Behavioral tests and levels of the cytokine mRNAs and proteins as well as glial cell activity were following the L5 SNT.

Results: In the CON group, TNF-α and IL-1β mRNA levels increased immediately after SNT and remained high for 6 d, while IL-6 transcripts only began to increase after 12 h. TNF-α and IL-1β mRNA levels in the COCK group were lower than in the CON group at all time points (P < 0.05). In the behavioral tests, allodynia and hyperalgesia were significantly lower in the COCK group from 2 d after SNT (P < 0.05).

Conclusions: The time courses of TNF-α, IL-6 and IL-1β mRNA expression after L5 SNT differ. RNA interference may be a method of reducing the development of mechanical allodynia and hyperalgesia in response to nerve injury.

No MeSH data available.


Related in: MedlinePlus

Glial fibrillary acidic protein (GFAP) expression over time in the CON (A) and COCK (B) groups. Astrocytes is determined at 4, 8, 12 h, 1, 2, 4 and 6 d after L5 SNT. BASE: 7 d after intrathecal catheter implantation without administration of siRNA or SNT operation (n = 3 per time point, occasional star-shape astrocytes moderately labeled by GFAP are much more common than in COCK group.
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Figure 7: Glial fibrillary acidic protein (GFAP) expression over time in the CON (A) and COCK (B) groups. Astrocytes is determined at 4, 8, 12 h, 1, 2, 4 and 6 d after L5 SNT. BASE: 7 d after intrathecal catheter implantation without administration of siRNA or SNT operation (n = 3 per time point, occasional star-shape astrocytes moderately labeled by GFAP are much more common than in COCK group.

Mentions: The time-course of GFAP and OX-42 expression in the L5 spinal cords of the CON and COCK groups are shown in Figs. 7 and 8, respectively. Taken as a whole, the CON group exhibited greater GFAP immunoreactivity than the COCK group. This was especially clear at 6 d after SNT. Proinflammatory effects were also revealed by enhanced immunostaining for OX-42.


The time-course and RNA interference of TNF-α, IL-6, and IL-1β expression on neuropathic pain induced by L5 spinal nerve transection in rats.

Choi BM, Lee SH, An SM, Park do Y, Lee GW, Noh GJ - Korean J Anesthesiol (2015)

Glial fibrillary acidic protein (GFAP) expression over time in the CON (A) and COCK (B) groups. Astrocytes is determined at 4, 8, 12 h, 1, 2, 4 and 6 d after L5 SNT. BASE: 7 d after intrathecal catheter implantation without administration of siRNA or SNT operation (n = 3 per time point, occasional star-shape astrocytes moderately labeled by GFAP are much more common than in COCK group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384404&req=5

Figure 7: Glial fibrillary acidic protein (GFAP) expression over time in the CON (A) and COCK (B) groups. Astrocytes is determined at 4, 8, 12 h, 1, 2, 4 and 6 d after L5 SNT. BASE: 7 d after intrathecal catheter implantation without administration of siRNA or SNT operation (n = 3 per time point, occasional star-shape astrocytes moderately labeled by GFAP are much more common than in COCK group.
Mentions: The time-course of GFAP and OX-42 expression in the L5 spinal cords of the CON and COCK groups are shown in Figs. 7 and 8, respectively. Taken as a whole, the CON group exhibited greater GFAP immunoreactivity than the COCK group. This was especially clear at 6 d after SNT. Proinflammatory effects were also revealed by enhanced immunostaining for OX-42.

Bottom Line: Behavioral tests and levels of the cytokine mRNAs and proteins as well as glial cell activity were following the L5 SNT.In the behavioral tests, allodynia and hyperalgesia were significantly lower in the COCK group from 2 d after SNT (P < 0.05).RNA interference may be a method of reducing the development of mechanical allodynia and hyperalgesia in response to nerve injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Pain Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea.

ABSTRACT

Background: The objective of this study was to investigate the time-course of the expression of TNF-α, IL-6, and IL-1β after L5 spinal nerve transection (SNT), and to determine the effect of small interfering RNA (siRNA) targeting these cytokines on neuropathic pain.

Methods: Rats received control siRNA (CON group, n = 80) or a cocktail of siRNAs targeting these cytokines (COCK group, n = 70). The siRNAs were given via intrathecal catheter 1 d prior to SNT, on the operation day, and 1, 2 and 3 d postoperatively. Behavioral tests and levels of the cytokine mRNAs and proteins as well as glial cell activity were following the L5 SNT.

Results: In the CON group, TNF-α and IL-1β mRNA levels increased immediately after SNT and remained high for 6 d, while IL-6 transcripts only began to increase after 12 h. TNF-α and IL-1β mRNA levels in the COCK group were lower than in the CON group at all time points (P < 0.05). In the behavioral tests, allodynia and hyperalgesia were significantly lower in the COCK group from 2 d after SNT (P < 0.05).

Conclusions: The time courses of TNF-α, IL-6 and IL-1β mRNA expression after L5 SNT differ. RNA interference may be a method of reducing the development of mechanical allodynia and hyperalgesia in response to nerve injury.

No MeSH data available.


Related in: MedlinePlus