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Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.

Tukappa N K A, Londonkar RL, Nayaka HB, Kumar C B S - Biol. Res. (2015)

Bottom Line: Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver.The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups.The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 μg/ml were not cytotoxic and appears to be safe.

View Article: PubMed Central - PubMed

ABSTRACT

Background: To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of SGOT (Serum glutamic oxaloacetic transaminase), SGPT (Serum glutamic pyruvic transaminase), ALP (Alkaline phosphatase), TP (Total protein), TB (Total bilirubin) and SOD (Superoxide dismutase), CAT (Catalase), MDA (Malondialdehyde). The cytotoxicity of the same extract on HepG2 cell lines were also assessed using MTT assay method at the concentration of 62.5, 125, 250, 500 μg/ml.

Results: Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver. The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups. Histopathological investigation further corroborated these biochemical observations. The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 μg/ml were not cytotoxic and appears to be safe.

Conclusions: Rumex vesicarius L. whole plant methanol extract exhibit hepatoprotective activity. However the cytotoxicity in HepG2 is inexplicable and warrants further study.

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Related in: MedlinePlus

Effect of methanolic extract ofRumex vesicariusL on acute liver injury induced by CCl4.A: (control): liver section with normal structure and architecture. B: (CCl4 treated): showing extensive area of necrosis, profound inflammation and congestion. C: (pretreatment with silymarin): reduced inflammation and degenerative changes. D: (pretreatment of extract at low dose of 100 mg/kgbw): reduced inflammation, degenerative changes. E: (pretreatment of extract at low dose of 200mg/kgbw): reduced inflammation, degenerative changes. cv: central vein
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Fig3: Effect of methanolic extract ofRumex vesicariusL on acute liver injury induced by CCl4.A: (control): liver section with normal structure and architecture. B: (CCl4 treated): showing extensive area of necrosis, profound inflammation and congestion. C: (pretreatment with silymarin): reduced inflammation and degenerative changes. D: (pretreatment of extract at low dose of 100 mg/kgbw): reduced inflammation, degenerative changes. E: (pretreatment of extract at low dose of 200mg/kgbw): reduced inflammation, degenerative changes. cv: central vein

Mentions: The results of histopathological studies provided supportive evidence for biochemical analysis. Histology of liver section of normal control animal exhibit normal hepatic cells each with well defined cytoplasm, prominent nucleus and nucleolus and well brought central vein (Figure 3A). The CCl4 intoxicated group animals showed total loss of hepatic architecture with centrilobular hepatic necrosis, fatty changes vacuolization and congestion of sinusoids, kupffer cell hyperplasia, crowding of central vein and apoptosis (Figure 3B). Pretreatment with Rumex vesicarius L. at the dose of 100 mg/kgbw and 200 mg/kgbw and silymarin showed protecting activity (Figure 3C-E). The methanol extract of Rumex vesicarius L. at low dose of 100 mg/kg bw. showed moderate or weak hepatoprotective activity for CCl4 injury. However the methanol extract at the high dose of 200 mg/kg bw and silymarin had shown a potential hepatoprotective activity and reduced the degenerative changes in liver. The result thus obtained is in the agreement with the work carried out by Sahreen [26]. They reported the hepatoprotective activity of methanol extract of Carissa opaca leaves on CCl4 induced damage in rat.Figure 3


Cytotoxicity and hepatoprotective attributes of methanolic extract of Rumex vesicarius L.

Tukappa N K A, Londonkar RL, Nayaka HB, Kumar C B S - Biol. Res. (2015)

Effect of methanolic extract ofRumex vesicariusL on acute liver injury induced by CCl4.A: (control): liver section with normal structure and architecture. B: (CCl4 treated): showing extensive area of necrosis, profound inflammation and congestion. C: (pretreatment with silymarin): reduced inflammation and degenerative changes. D: (pretreatment of extract at low dose of 100 mg/kgbw): reduced inflammation, degenerative changes. E: (pretreatment of extract at low dose of 200mg/kgbw): reduced inflammation, degenerative changes. cv: central vein
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4384386&req=5

Fig3: Effect of methanolic extract ofRumex vesicariusL on acute liver injury induced by CCl4.A: (control): liver section with normal structure and architecture. B: (CCl4 treated): showing extensive area of necrosis, profound inflammation and congestion. C: (pretreatment with silymarin): reduced inflammation and degenerative changes. D: (pretreatment of extract at low dose of 100 mg/kgbw): reduced inflammation, degenerative changes. E: (pretreatment of extract at low dose of 200mg/kgbw): reduced inflammation, degenerative changes. cv: central vein
Mentions: The results of histopathological studies provided supportive evidence for biochemical analysis. Histology of liver section of normal control animal exhibit normal hepatic cells each with well defined cytoplasm, prominent nucleus and nucleolus and well brought central vein (Figure 3A). The CCl4 intoxicated group animals showed total loss of hepatic architecture with centrilobular hepatic necrosis, fatty changes vacuolization and congestion of sinusoids, kupffer cell hyperplasia, crowding of central vein and apoptosis (Figure 3B). Pretreatment with Rumex vesicarius L. at the dose of 100 mg/kgbw and 200 mg/kgbw and silymarin showed protecting activity (Figure 3C-E). The methanol extract of Rumex vesicarius L. at low dose of 100 mg/kg bw. showed moderate or weak hepatoprotective activity for CCl4 injury. However the methanol extract at the high dose of 200 mg/kg bw and silymarin had shown a potential hepatoprotective activity and reduced the degenerative changes in liver. The result thus obtained is in the agreement with the work carried out by Sahreen [26]. They reported the hepatoprotective activity of methanol extract of Carissa opaca leaves on CCl4 induced damage in rat.Figure 3

Bottom Line: Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver.The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups.The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 μg/ml were not cytotoxic and appears to be safe.

View Article: PubMed Central - PubMed

ABSTRACT

Background: To evaluate the hepatoprotective potential and invitro cytotoxicity studies of whole plant methanol extract of Rumex vesicarius L. Methanol extract at a dose of 100 mg/kg bw and 200 mg/kg bw were assessed for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of SGOT (Serum glutamic oxaloacetic transaminase), SGPT (Serum glutamic pyruvic transaminase), ALP (Alkaline phosphatase), TP (Total protein), TB (Total bilirubin) and SOD (Superoxide dismutase), CAT (Catalase), MDA (Malondialdehyde). The cytotoxicity of the same extract on HepG2 cell lines were also assessed using MTT assay method at the concentration of 62.5, 125, 250, 500 μg/ml.

Results: Pretreatment of animals with whole plant methanol extracts of Rumex vesicarius L. significantly reduced the liver damage and the symptoms of liver injury by restoration of architecture of liver. The biochemical parameters in serum also improved in treated groups compared to the control and standard (silymarin) groups. Histopathological investigation further corroborated these biochemical observations. The cytotoxicity results indicated that the plant extract which were inhibitory to the proliferation of HepG2 cell line with IC50 value of 563.33 ± 0.8 μg/ml were not cytotoxic and appears to be safe.

Conclusions: Rumex vesicarius L. whole plant methanol extract exhibit hepatoprotective activity. However the cytotoxicity in HepG2 is inexplicable and warrants further study.

Show MeSH
Related in: MedlinePlus