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CK19 is a sensitive marker for yolk sac tumours of the testis.

Bremmer F, Ströbel P, Jarry H, Strecker J, Gaisa N, Strauß A, Schweyer S, Radzun HJ, Behnes CL - Diagn Pathol (2015)

Bottom Line: They are histologically divided into seminomas and non-seminomas.In addition Leydig cell- (n = 10) and Sertoli cell- tumours (n = 4) were included in this study.All investigated seminomas, embryonic carcinomas as well as normal testis and intratubular germ cell neoplasias did not express CK19.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathology, University Medical Center, University of Göttingen, Göttingen, Germany. felix.bremmer@med.uni-goettingen.de.

ABSTRACT

Background: Malignant germ cell tumours are the most common malignant tumours in young men. They are histologically divided into seminomas and non-seminomas. Non-seminomas are further subdivided into embryonic carcinomas, yolk sac tumours, chorionic carcinomas, and teratomas. For the therapeutic management it is essential to differentiate between these histological subtypes.

Methods: Investigated cases included normal testis (n = 50), intratubular germ cell neoplasia (n = 25), seminomas (n = 67), embryonic carcinomas (n = 56), yolk sac tumours (n = 29), chorionic carcinomas (n = 2), teratomas (n = 7) and four metastases of YST's for their CK19 expression. In addition Leydig cell- (n = 10) and Sertoli cell- tumours (n = 4) were included in this study.

Results: All investigated seminomas, embryonic carcinomas as well as normal testis and intratubular germ cell neoplasias did not express CK19. In contrast, all investigated yolk sac tumours strongly expressed CK19 protein. These findings became also evident in mixed germ cell tumours consisting of embryonic carcinomas and yolk sac tumours, although CK19-expression could also be observed in analysed chorionic carcinomas and epithelial components of teratomas.

Conclusion: CK19 proved to be a sensitive marker to identify yolk sac tumours of the testis and to distinguish them from other germ cell tumours, especially seminomas and embryonic carcinomas.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4075546891400979.

No MeSH data available.


Related in: MedlinePlus

CK19-expression in mixed germ cell tumours of the testis and metastases. YST showing a strong expression of CK19 and embryonic carcinoma component in the same tumour does not express CK19 protein (A and B, x100). Omentum majus with metastasis of an YST (C, HE staining x40) expressing CK19 protein (D, x40). Pulmonary metastases of an YST of the testis (E, HE staining x40) express CK19 (F, x40). Intestinal mucosa in teratomas (G, x40+ H, x100) shows positive CK19-expression.
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Fig3: CK19-expression in mixed germ cell tumours of the testis and metastases. YST showing a strong expression of CK19 and embryonic carcinoma component in the same tumour does not express CK19 protein (A and B, x100). Omentum majus with metastasis of an YST (C, HE staining x40) expressing CK19 protein (D, x40). Pulmonary metastases of an YST of the testis (E, HE staining x40) express CK19 (F, x40). Intestinal mucosa in teratomas (G, x40+ H, x100) shows positive CK19-expression.

Mentions: All 29 YST strongly expressed Glypican-3 and CK19. The immunohistochemical signals were located within the cytoplasma and at the cell membrane (Figure 1 E-G). In contrast, only 18 of these tumours showed a patchy expression of AFP (Figure 1H). CK19 expression was evident in the different growth patterns of YST such as reticular/microcystic pattern (Figure 2A and B), solid pattern (Figure 2C and D), papillary pattern (Figure 2E and F) or endodermal sinus pattern with festooned appearance (Figure 2G and H). The CK19 positivity could also be shown in tumours consisting of YST and embryonic carcinomas (Figure 3A and B) as well as in metastasis of the omentum majus (Figure 3C and D), lung (Figure 3E and F) and lymph nodes (data not shown).Figure 2


CK19 is a sensitive marker for yolk sac tumours of the testis.

Bremmer F, Ströbel P, Jarry H, Strecker J, Gaisa N, Strauß A, Schweyer S, Radzun HJ, Behnes CL - Diagn Pathol (2015)

CK19-expression in mixed germ cell tumours of the testis and metastases. YST showing a strong expression of CK19 and embryonic carcinoma component in the same tumour does not express CK19 protein (A and B, x100). Omentum majus with metastasis of an YST (C, HE staining x40) expressing CK19 protein (D, x40). Pulmonary metastases of an YST of the testis (E, HE staining x40) express CK19 (F, x40). Intestinal mucosa in teratomas (G, x40+ H, x100) shows positive CK19-expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4384355&req=5

Fig3: CK19-expression in mixed germ cell tumours of the testis and metastases. YST showing a strong expression of CK19 and embryonic carcinoma component in the same tumour does not express CK19 protein (A and B, x100). Omentum majus with metastasis of an YST (C, HE staining x40) expressing CK19 protein (D, x40). Pulmonary metastases of an YST of the testis (E, HE staining x40) express CK19 (F, x40). Intestinal mucosa in teratomas (G, x40+ H, x100) shows positive CK19-expression.
Mentions: All 29 YST strongly expressed Glypican-3 and CK19. The immunohistochemical signals were located within the cytoplasma and at the cell membrane (Figure 1 E-G). In contrast, only 18 of these tumours showed a patchy expression of AFP (Figure 1H). CK19 expression was evident in the different growth patterns of YST such as reticular/microcystic pattern (Figure 2A and B), solid pattern (Figure 2C and D), papillary pattern (Figure 2E and F) or endodermal sinus pattern with festooned appearance (Figure 2G and H). The CK19 positivity could also be shown in tumours consisting of YST and embryonic carcinomas (Figure 3A and B) as well as in metastasis of the omentum majus (Figure 3C and D), lung (Figure 3E and F) and lymph nodes (data not shown).Figure 2

Bottom Line: They are histologically divided into seminomas and non-seminomas.In addition Leydig cell- (n = 10) and Sertoli cell- tumours (n = 4) were included in this study.All investigated seminomas, embryonic carcinomas as well as normal testis and intratubular germ cell neoplasias did not express CK19.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathology, University Medical Center, University of Göttingen, Göttingen, Germany. felix.bremmer@med.uni-goettingen.de.

ABSTRACT

Background: Malignant germ cell tumours are the most common malignant tumours in young men. They are histologically divided into seminomas and non-seminomas. Non-seminomas are further subdivided into embryonic carcinomas, yolk sac tumours, chorionic carcinomas, and teratomas. For the therapeutic management it is essential to differentiate between these histological subtypes.

Methods: Investigated cases included normal testis (n = 50), intratubular germ cell neoplasia (n = 25), seminomas (n = 67), embryonic carcinomas (n = 56), yolk sac tumours (n = 29), chorionic carcinomas (n = 2), teratomas (n = 7) and four metastases of YST's for their CK19 expression. In addition Leydig cell- (n = 10) and Sertoli cell- tumours (n = 4) were included in this study.

Results: All investigated seminomas, embryonic carcinomas as well as normal testis and intratubular germ cell neoplasias did not express CK19. In contrast, all investigated yolk sac tumours strongly expressed CK19 protein. These findings became also evident in mixed germ cell tumours consisting of embryonic carcinomas and yolk sac tumours, although CK19-expression could also be observed in analysed chorionic carcinomas and epithelial components of teratomas.

Conclusion: CK19 proved to be a sensitive marker to identify yolk sac tumours of the testis and to distinguish them from other germ cell tumours, especially seminomas and embryonic carcinomas.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4075546891400979.

No MeSH data available.


Related in: MedlinePlus