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CK19 is a sensitive marker for yolk sac tumours of the testis.

Bremmer F, Ströbel P, Jarry H, Strecker J, Gaisa N, Strauß A, Schweyer S, Radzun HJ, Behnes CL - Diagn Pathol (2015)

Bottom Line: In addition Leydig cell- (n = 10) and Sertoli cell- tumours (n = 4) were included in this study.All investigated seminomas, embryonic carcinomas as well as normal testis and intratubular germ cell neoplasias did not express CK19.These findings became also evident in mixed germ cell tumours consisting of embryonic carcinomas and yolk sac tumours, although CK19-expression could also be observed in analysed chorionic carcinomas and epithelial components of teratomas.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathology, University Medical Center, University of Göttingen, Göttingen, Germany. felix.bremmer@med.uni-goettingen.de.

ABSTRACT

Background: Malignant germ cell tumours are the most common malignant tumours in young men. They are histologically divided into seminomas and non-seminomas. Non-seminomas are further subdivided into embryonic carcinomas, yolk sac tumours, chorionic carcinomas, and teratomas. For the therapeutic management it is essential to differentiate between these histological subtypes.

Methods: Investigated cases included normal testis (n = 50), intratubular germ cell neoplasia (n = 25), seminomas (n = 67), embryonic carcinomas (n = 56), yolk sac tumours (n = 29), chorionic carcinomas (n = 2), teratomas (n = 7) and four metastases of YST's for their CK19 expression. In addition Leydig cell- (n = 10) and Sertoli cell- tumours (n = 4) were included in this study.

Results: All investigated seminomas, embryonic carcinomas as well as normal testis and intratubular germ cell neoplasias did not express CK19. In contrast, all investigated yolk sac tumours strongly expressed CK19 protein. These findings became also evident in mixed germ cell tumours consisting of embryonic carcinomas and yolk sac tumours, although CK19-expression could also be observed in analysed chorionic carcinomas and epithelial components of teratomas.

Conclusion: CK19 proved to be a sensitive marker to identify yolk sac tumours of the testis and to distinguish them from other germ cell tumours, especially seminomas and embryonic carcinomas.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4075546891400979.

No MeSH data available.


Related in: MedlinePlus

CK19-expression in normal testis and germ cell tumours of the testis. CK19 is not expressed in normal testis (A, x40), IGCNU (B, x40), seminoma (C, x100), and embryonic carcinoma (D, x100). In all cases the rete testis showed a strong cytoplasmic and membrane bound expression of CK19 (A-D). YST of the testis with microcystic pattern (E, HE staining x200) express CK19 (F, x100), Glypican-3 (G, x100), and AFP (H, x100).
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Fig1: CK19-expression in normal testis and germ cell tumours of the testis. CK19 is not expressed in normal testis (A, x40), IGCNU (B, x40), seminoma (C, x100), and embryonic carcinoma (D, x100). In all cases the rete testis showed a strong cytoplasmic and membrane bound expression of CK19 (A-D). YST of the testis with microcystic pattern (E, HE staining x200) express CK19 (F, x100), Glypican-3 (G, x100), and AFP (H, x100).

Mentions: Normal testis, cells of the interstitium and Leydig cells did not express CK19 (Figure 1A), Glypican-3, or AFP. In addition IGCNU (Figure 1B), all examined seminomas (Figure 1C) and embryonic carcinomas (Figure 1D) did not express CK19, Glypican-3, or AFP. In all examined tissues the rete testis and the epidymides showed a strong cytoplasmatic and membrane bound expression of CK19 protein (Figure 1 A-D).Figure 1


CK19 is a sensitive marker for yolk sac tumours of the testis.

Bremmer F, Ströbel P, Jarry H, Strecker J, Gaisa N, Strauß A, Schweyer S, Radzun HJ, Behnes CL - Diagn Pathol (2015)

CK19-expression in normal testis and germ cell tumours of the testis. CK19 is not expressed in normal testis (A, x40), IGCNU (B, x40), seminoma (C, x100), and embryonic carcinoma (D, x100). In all cases the rete testis showed a strong cytoplasmic and membrane bound expression of CK19 (A-D). YST of the testis with microcystic pattern (E, HE staining x200) express CK19 (F, x100), Glypican-3 (G, x100), and AFP (H, x100).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4384355&req=5

Fig1: CK19-expression in normal testis and germ cell tumours of the testis. CK19 is not expressed in normal testis (A, x40), IGCNU (B, x40), seminoma (C, x100), and embryonic carcinoma (D, x100). In all cases the rete testis showed a strong cytoplasmic and membrane bound expression of CK19 (A-D). YST of the testis with microcystic pattern (E, HE staining x200) express CK19 (F, x100), Glypican-3 (G, x100), and AFP (H, x100).
Mentions: Normal testis, cells of the interstitium and Leydig cells did not express CK19 (Figure 1A), Glypican-3, or AFP. In addition IGCNU (Figure 1B), all examined seminomas (Figure 1C) and embryonic carcinomas (Figure 1D) did not express CK19, Glypican-3, or AFP. In all examined tissues the rete testis and the epidymides showed a strong cytoplasmatic and membrane bound expression of CK19 protein (Figure 1 A-D).Figure 1

Bottom Line: In addition Leydig cell- (n = 10) and Sertoli cell- tumours (n = 4) were included in this study.All investigated seminomas, embryonic carcinomas as well as normal testis and intratubular germ cell neoplasias did not express CK19.These findings became also evident in mixed germ cell tumours consisting of embryonic carcinomas and yolk sac tumours, although CK19-expression could also be observed in analysed chorionic carcinomas and epithelial components of teratomas.

View Article: PubMed Central - PubMed

Affiliation: Institute of Pathology, University Medical Center, University of Göttingen, Göttingen, Germany. felix.bremmer@med.uni-goettingen.de.

ABSTRACT

Background: Malignant germ cell tumours are the most common malignant tumours in young men. They are histologically divided into seminomas and non-seminomas. Non-seminomas are further subdivided into embryonic carcinomas, yolk sac tumours, chorionic carcinomas, and teratomas. For the therapeutic management it is essential to differentiate between these histological subtypes.

Methods: Investigated cases included normal testis (n = 50), intratubular germ cell neoplasia (n = 25), seminomas (n = 67), embryonic carcinomas (n = 56), yolk sac tumours (n = 29), chorionic carcinomas (n = 2), teratomas (n = 7) and four metastases of YST's for their CK19 expression. In addition Leydig cell- (n = 10) and Sertoli cell- tumours (n = 4) were included in this study.

Results: All investigated seminomas, embryonic carcinomas as well as normal testis and intratubular germ cell neoplasias did not express CK19. In contrast, all investigated yolk sac tumours strongly expressed CK19 protein. These findings became also evident in mixed germ cell tumours consisting of embryonic carcinomas and yolk sac tumours, although CK19-expression could also be observed in analysed chorionic carcinomas and epithelial components of teratomas.

Conclusion: CK19 proved to be a sensitive marker to identify yolk sac tumours of the testis and to distinguish them from other germ cell tumours, especially seminomas and embryonic carcinomas.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4075546891400979.

No MeSH data available.


Related in: MedlinePlus