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Crude extract of hydatid laminated layer from Echinococcus granulosus cyst attenuates mucosal intestinal damage and inflammatory responses in Dextran Sulfate Sodium induced colitis in mice.

Soufli I, Toumi R, Rafa H, Amri M, Labsi M, Khelifi L, Nicoletti F, Touil-Boukoffa C - J Inflamm (Lond) (2015)

Bottom Line: These results were associated with localized downregulation of iNOS and NF-κB expression.Our results demonstrate the potent anti-inflammatory effects of hydatid laminated layer.Furthermore, preventive treatment with the laminated layer played a beneficial role in maintaining the integrity of the intestinal mucosal barrier against DSS-induced injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Laboratory of Cellular and Molecular Biology, University of Sciences and Technology Houari Boumediene USTHB, Algiers, Algeria.

ABSTRACT

Background: Inflammatory bowel disease is an immunologically mediated disease. Notably, it is less common in countries where there is a greater risk of exposure to helminths. In our study, we examined the modulatory effect of the laminated layer extracted from the cyst wall of a helminth parasite, Echinococcus granulosus, on dextran sulfate sodium (DSS)-induced colitis in mice.

Methods: An acute colitis was induced in BALB/c mice using 2.5% w/v DSS in drinking water. The crude extract of E. granulosus laminated layer was injected intraperitoneally daily, starting 3 days before colitis induction. The Disease Activity Index was monitored daily, colon length and weight were measured and histological scores were evaluated. Nitric oxide (NO) and cytokine levels (interferon γ (IFN-γ), tumor necrosis factor α (TNF-α) and interleukin 10 (IL-10)) were assessed by enzyme-linked immunosorbent assay. In addition, the colonic expression of inducible nitric oxide synthase (iNOS) and nuclear factor-κB (NF-κB) was examined. Statistical analyses were performed by one-way analysis of variance and the survival rate was analyzed by the long rank test.

Results: Hydatid laminated layer pretreatment significantly improved the clinical symptoms and histological scores (*** p < 0.01) observed during DSS-induced colitis and maintained mucus production by goblet cells. Furthermore, treatment with hydatid laminated layer caused a significant decrease in NO, IFN-γ (** p < 0.01) and TNF-α production (* p < 0.05) and an increase in IL-10 production. These results were associated with localized downregulation of iNOS and NF-κB expression.

Conclusions: Our results demonstrate the potent anti-inflammatory effects of hydatid laminated layer. Furthermore, preventive treatment with the laminated layer played a beneficial role in maintaining the integrity of the intestinal mucosal barrier against DSS-induced injury.

No MeSH data available.


Related in: MedlinePlus

Representative images of immunofluorescence staining. Distal colon tissues from mice were stained with anti-iNOS (a–d) and anti p65 NF-κB subunit (e–h). Colonic tissue stained with anti-iNOS (a) CTRL group, (b) DSS group, (c) LL/DSS group, (d) LL group. Colonic tissue stained with anti-p65 subunit NF-κB (e) CTRL group, (f) DSS group, (g) LL/DSS group, (h) LL group. Red arrow indicates iNOS or NF-κB staining, all magnifications are × 40.
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Fig7: Representative images of immunofluorescence staining. Distal colon tissues from mice were stained with anti-iNOS (a–d) and anti p65 NF-κB subunit (e–h). Colonic tissue stained with anti-iNOS (a) CTRL group, (b) DSS group, (c) LL/DSS group, (d) LL group. Colonic tissue stained with anti-p65 subunit NF-κB (e) CTRL group, (f) DSS group, (g) LL/DSS group, (h) LL group. Red arrow indicates iNOS or NF-κB staining, all magnifications are × 40.

Mentions: High expression of iNOS is considered the most important source of elevated nitrite levels in experimental colitis and in patients with IBD [4-7]. The expression of iNOS is transcriptionally regulated by NF-κB. Therefore, we examined the effect of crude extract of hydatid LL on iNOS and NF-κB expression in DSS-induced colitis in mice. Colon tissue sections were tested for the expression of iNOS and NF-κB by immunofluorescence using anti-iNOS and anti-NF-κB p65 subunit antibodies. The expression of both NF-κB and iNOS was markedly increased in the colonic mucosa of DSS treated mice in comparison with the untreated control group. This increase in expression in colonic mucosa was significantly attenuated by LL treatment (Figure 7).Figure 7


Crude extract of hydatid laminated layer from Echinococcus granulosus cyst attenuates mucosal intestinal damage and inflammatory responses in Dextran Sulfate Sodium induced colitis in mice.

Soufli I, Toumi R, Rafa H, Amri M, Labsi M, Khelifi L, Nicoletti F, Touil-Boukoffa C - J Inflamm (Lond) (2015)

Representative images of immunofluorescence staining. Distal colon tissues from mice were stained with anti-iNOS (a–d) and anti p65 NF-κB subunit (e–h). Colonic tissue stained with anti-iNOS (a) CTRL group, (b) DSS group, (c) LL/DSS group, (d) LL group. Colonic tissue stained with anti-p65 subunit NF-κB (e) CTRL group, (f) DSS group, (g) LL/DSS group, (h) LL group. Red arrow indicates iNOS or NF-κB staining, all magnifications are × 40.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4384302&req=5

Fig7: Representative images of immunofluorescence staining. Distal colon tissues from mice were stained with anti-iNOS (a–d) and anti p65 NF-κB subunit (e–h). Colonic tissue stained with anti-iNOS (a) CTRL group, (b) DSS group, (c) LL/DSS group, (d) LL group. Colonic tissue stained with anti-p65 subunit NF-κB (e) CTRL group, (f) DSS group, (g) LL/DSS group, (h) LL group. Red arrow indicates iNOS or NF-κB staining, all magnifications are × 40.
Mentions: High expression of iNOS is considered the most important source of elevated nitrite levels in experimental colitis and in patients with IBD [4-7]. The expression of iNOS is transcriptionally regulated by NF-κB. Therefore, we examined the effect of crude extract of hydatid LL on iNOS and NF-κB expression in DSS-induced colitis in mice. Colon tissue sections were tested for the expression of iNOS and NF-κB by immunofluorescence using anti-iNOS and anti-NF-κB p65 subunit antibodies. The expression of both NF-κB and iNOS was markedly increased in the colonic mucosa of DSS treated mice in comparison with the untreated control group. This increase in expression in colonic mucosa was significantly attenuated by LL treatment (Figure 7).Figure 7

Bottom Line: These results were associated with localized downregulation of iNOS and NF-κB expression.Our results demonstrate the potent anti-inflammatory effects of hydatid laminated layer.Furthermore, preventive treatment with the laminated layer played a beneficial role in maintaining the integrity of the intestinal mucosal barrier against DSS-induced injury.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Laboratory of Cellular and Molecular Biology, University of Sciences and Technology Houari Boumediene USTHB, Algiers, Algeria.

ABSTRACT

Background: Inflammatory bowel disease is an immunologically mediated disease. Notably, it is less common in countries where there is a greater risk of exposure to helminths. In our study, we examined the modulatory effect of the laminated layer extracted from the cyst wall of a helminth parasite, Echinococcus granulosus, on dextran sulfate sodium (DSS)-induced colitis in mice.

Methods: An acute colitis was induced in BALB/c mice using 2.5% w/v DSS in drinking water. The crude extract of E. granulosus laminated layer was injected intraperitoneally daily, starting 3 days before colitis induction. The Disease Activity Index was monitored daily, colon length and weight were measured and histological scores were evaluated. Nitric oxide (NO) and cytokine levels (interferon γ (IFN-γ), tumor necrosis factor α (TNF-α) and interleukin 10 (IL-10)) were assessed by enzyme-linked immunosorbent assay. In addition, the colonic expression of inducible nitric oxide synthase (iNOS) and nuclear factor-κB (NF-κB) was examined. Statistical analyses were performed by one-way analysis of variance and the survival rate was analyzed by the long rank test.

Results: Hydatid laminated layer pretreatment significantly improved the clinical symptoms and histological scores (*** p < 0.01) observed during DSS-induced colitis and maintained mucus production by goblet cells. Furthermore, treatment with hydatid laminated layer caused a significant decrease in NO, IFN-γ (** p < 0.01) and TNF-α production (* p < 0.05) and an increase in IL-10 production. These results were associated with localized downregulation of iNOS and NF-κB expression.

Conclusions: Our results demonstrate the potent anti-inflammatory effects of hydatid laminated layer. Furthermore, preventive treatment with the laminated layer played a beneficial role in maintaining the integrity of the intestinal mucosal barrier against DSS-induced injury.

No MeSH data available.


Related in: MedlinePlus