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Experimental study on cryotherapy for fungal corneal ulcer.

Chen Y, Yang W, Gao M, Belin MW, Yu H, Yu J - BMC Ophthalmol (2015)

Bottom Line: Different fungi showed different susceptibilities to treatment.The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.This treatment could help facilitate the practice of fungal keratitis treatment in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, General Hospital of Shenyang Military Area Command, No. 83 Wenhua Road, Shenhe District, Shenyang, 110840, China. shuchiwsw@gmail.com.

ABSTRACT

Background: Fungal corneal ulcer is one of the major causes of visual impairment worldwide. Treatment of fungal corneal ulcer mainly depends on anti-fungal agents. In the current study, we developed an integrated combination therapy of cryotherapy and anti-fungal agents to facilitate effective treatment of fungal corneal ulcer.

Methods: Rabbit models of cornea infection were established using a combined method of intrastromal injection and keratoplasty. After treatment with cryotherapy and anti-fungal agents, scanning electron microscopy, transmission electron microscopy, and confocal microscopy were conducted to observe changes in microstructure in the rabbits. Periodic acid Schiff A and hematoxylin and eosin staining were used for detection of histological changes.

Results: Continuous scanning electron microscopy and transmission electron microscopy observations showed that cryothermal treatment inhibited growth of fungal mycelium by destroying fungal cellular structures. Typical cryotherapy was effective in curing fungal corneal ulcer. Different fungi showed different susceptibilities to treatment. The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.

Conclusions: Our study provides a novel method of a combination of cryotherapy and anti-fungal agents for treatment of fungal corneal ulcer. This treatment could help facilitate the practice of fungal keratitis treatment in the future.

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Related in: MedlinePlus

Effect of cryotherapy on left eye of rabbit fungal corneal ulcer model by confocal microscopy. On the 1st day in the control group, there was obvious light reflection and diffuse hyphae in the area of the nidus. On the 30th day in the control group, there was a small amount of hyphae in the area of the nidus. On the 1st day in the surgery group, there was obvious light reflection and diffuse mycelia in the area of the nidus. On the 15th day in the surgery group, there was less light reflection and fewer inflammatory cells. On the 30th day, no or a small amount of hyphae were present.
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Fig5: Effect of cryotherapy on left eye of rabbit fungal corneal ulcer model by confocal microscopy. On the 1st day in the control group, there was obvious light reflection and diffuse hyphae in the area of the nidus. On the 30th day in the control group, there was a small amount of hyphae in the area of the nidus. On the 1st day in the surgery group, there was obvious light reflection and diffuse mycelia in the area of the nidus. On the 15th day in the surgery group, there was less light reflection and fewer inflammatory cells. On the 30th day, no or a small amount of hyphae were present.

Mentions: Slit-lamp microscopy showed that the combined therapy had a better treatment outcome for corneal ulcer compared with medication therapy. In the control group with different fungi, lesions did not improve until the 30th day after treatment (Figure 4). However, fungal corneal ulcers were not healed even at the 30th day after treatment, with visible symptoms in the corneas of the rabbits (Figure 4). In the surgery group with different fungi, treatment effect of cryotherapy was observed, with healing of fungal corneal ulcers since the 15th day after surgery (Figure 4). Confocal microscopy showed the same pattern as that with slit-lamp observations (Figure 5).Figure 4


Experimental study on cryotherapy for fungal corneal ulcer.

Chen Y, Yang W, Gao M, Belin MW, Yu H, Yu J - BMC Ophthalmol (2015)

Effect of cryotherapy on left eye of rabbit fungal corneal ulcer model by confocal microscopy. On the 1st day in the control group, there was obvious light reflection and diffuse hyphae in the area of the nidus. On the 30th day in the control group, there was a small amount of hyphae in the area of the nidus. On the 1st day in the surgery group, there was obvious light reflection and diffuse mycelia in the area of the nidus. On the 15th day in the surgery group, there was less light reflection and fewer inflammatory cells. On the 30th day, no or a small amount of hyphae were present.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4384297&req=5

Fig5: Effect of cryotherapy on left eye of rabbit fungal corneal ulcer model by confocal microscopy. On the 1st day in the control group, there was obvious light reflection and diffuse hyphae in the area of the nidus. On the 30th day in the control group, there was a small amount of hyphae in the area of the nidus. On the 1st day in the surgery group, there was obvious light reflection and diffuse mycelia in the area of the nidus. On the 15th day in the surgery group, there was less light reflection and fewer inflammatory cells. On the 30th day, no or a small amount of hyphae were present.
Mentions: Slit-lamp microscopy showed that the combined therapy had a better treatment outcome for corneal ulcer compared with medication therapy. In the control group with different fungi, lesions did not improve until the 30th day after treatment (Figure 4). However, fungal corneal ulcers were not healed even at the 30th day after treatment, with visible symptoms in the corneas of the rabbits (Figure 4). In the surgery group with different fungi, treatment effect of cryotherapy was observed, with healing of fungal corneal ulcers since the 15th day after surgery (Figure 4). Confocal microscopy showed the same pattern as that with slit-lamp observations (Figure 5).Figure 4

Bottom Line: Different fungi showed different susceptibilities to treatment.The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.This treatment could help facilitate the practice of fungal keratitis treatment in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, General Hospital of Shenyang Military Area Command, No. 83 Wenhua Road, Shenhe District, Shenyang, 110840, China. shuchiwsw@gmail.com.

ABSTRACT

Background: Fungal corneal ulcer is one of the major causes of visual impairment worldwide. Treatment of fungal corneal ulcer mainly depends on anti-fungal agents. In the current study, we developed an integrated combination therapy of cryotherapy and anti-fungal agents to facilitate effective treatment of fungal corneal ulcer.

Methods: Rabbit models of cornea infection were established using a combined method of intrastromal injection and keratoplasty. After treatment with cryotherapy and anti-fungal agents, scanning electron microscopy, transmission electron microscopy, and confocal microscopy were conducted to observe changes in microstructure in the rabbits. Periodic acid Schiff A and hematoxylin and eosin staining were used for detection of histological changes.

Results: Continuous scanning electron microscopy and transmission electron microscopy observations showed that cryothermal treatment inhibited growth of fungal mycelium by destroying fungal cellular structures. Typical cryotherapy was effective in curing fungal corneal ulcer. Different fungi showed different susceptibilities to treatment. The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.

Conclusions: Our study provides a novel method of a combination of cryotherapy and anti-fungal agents for treatment of fungal corneal ulcer. This treatment could help facilitate the practice of fungal keratitis treatment in the future.

Show MeSH
Related in: MedlinePlus