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Experimental study on cryotherapy for fungal corneal ulcer.

Chen Y, Yang W, Gao M, Belin MW, Yu H, Yu J - BMC Ophthalmol (2015)

Bottom Line: Different fungi showed different susceptibilities to treatment.The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.This treatment could help facilitate the practice of fungal keratitis treatment in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, General Hospital of Shenyang Military Area Command, No. 83 Wenhua Road, Shenhe District, Shenyang, 110840, China. shuchiwsw@gmail.com.

ABSTRACT

Background: Fungal corneal ulcer is one of the major causes of visual impairment worldwide. Treatment of fungal corneal ulcer mainly depends on anti-fungal agents. In the current study, we developed an integrated combination therapy of cryotherapy and anti-fungal agents to facilitate effective treatment of fungal corneal ulcer.

Methods: Rabbit models of cornea infection were established using a combined method of intrastromal injection and keratoplasty. After treatment with cryotherapy and anti-fungal agents, scanning electron microscopy, transmission electron microscopy, and confocal microscopy were conducted to observe changes in microstructure in the rabbits. Periodic acid Schiff A and hematoxylin and eosin staining were used for detection of histological changes.

Results: Continuous scanning electron microscopy and transmission electron microscopy observations showed that cryothermal treatment inhibited growth of fungal mycelium by destroying fungal cellular structures. Typical cryotherapy was effective in curing fungal corneal ulcer. Different fungi showed different susceptibilities to treatment. The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.

Conclusions: Our study provides a novel method of a combination of cryotherapy and anti-fungal agents for treatment of fungal corneal ulcer. This treatment could help facilitate the practice of fungal keratitis treatment in the future.

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Related in: MedlinePlus

Fungal structural changes before and after cryotherapy by TEM (8000×). After surgery, the cells of Candida albicans were irregularly spherical, and the cell wall and the cell membrane had disappeared. The integrity of the cells of Fusarium solani and Aspergillus fumigates was disrupted. Arrows indicate the detailed changes due to cryotherapy.
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Fig3: Fungal structural changes before and after cryotherapy by TEM (8000×). After surgery, the cells of Candida albicans were irregularly spherical, and the cell wall and the cell membrane had disappeared. The integrity of the cells of Fusarium solani and Aspergillus fumigates was disrupted. Arrows indicate the detailed changes due to cryotherapy.

Mentions: The results of TEM were consistent with the results of SEM. In Candida albicans Group, the cells of Candida albicans were irregularly spherical, and cell wall and the cell membrane had disappeared (Figure 3). In Groups Fusarium solani and Aspergillus fumigates, the cell wall and cell membrane were fractured after surgery and the structural integrity of the cells was disrupted (Figure 3).Figure 3


Experimental study on cryotherapy for fungal corneal ulcer.

Chen Y, Yang W, Gao M, Belin MW, Yu H, Yu J - BMC Ophthalmol (2015)

Fungal structural changes before and after cryotherapy by TEM (8000×). After surgery, the cells of Candida albicans were irregularly spherical, and the cell wall and the cell membrane had disappeared. The integrity of the cells of Fusarium solani and Aspergillus fumigates was disrupted. Arrows indicate the detailed changes due to cryotherapy.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4384297&req=5

Fig3: Fungal structural changes before and after cryotherapy by TEM (8000×). After surgery, the cells of Candida albicans were irregularly spherical, and the cell wall and the cell membrane had disappeared. The integrity of the cells of Fusarium solani and Aspergillus fumigates was disrupted. Arrows indicate the detailed changes due to cryotherapy.
Mentions: The results of TEM were consistent with the results of SEM. In Candida albicans Group, the cells of Candida albicans were irregularly spherical, and cell wall and the cell membrane had disappeared (Figure 3). In Groups Fusarium solani and Aspergillus fumigates, the cell wall and cell membrane were fractured after surgery and the structural integrity of the cells was disrupted (Figure 3).Figure 3

Bottom Line: Different fungi showed different susceptibilities to treatment.The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.This treatment could help facilitate the practice of fungal keratitis treatment in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, General Hospital of Shenyang Military Area Command, No. 83 Wenhua Road, Shenhe District, Shenyang, 110840, China. shuchiwsw@gmail.com.

ABSTRACT

Background: Fungal corneal ulcer is one of the major causes of visual impairment worldwide. Treatment of fungal corneal ulcer mainly depends on anti-fungal agents. In the current study, we developed an integrated combination therapy of cryotherapy and anti-fungal agents to facilitate effective treatment of fungal corneal ulcer.

Methods: Rabbit models of cornea infection were established using a combined method of intrastromal injection and keratoplasty. After treatment with cryotherapy and anti-fungal agents, scanning electron microscopy, transmission electron microscopy, and confocal microscopy were conducted to observe changes in microstructure in the rabbits. Periodic acid Schiff A and hematoxylin and eosin staining were used for detection of histological changes.

Results: Continuous scanning electron microscopy and transmission electron microscopy observations showed that cryothermal treatment inhibited growth of fungal mycelium by destroying fungal cellular structures. Typical cryotherapy was effective in curing fungal corneal ulcer. Different fungi showed different susceptibilities to treatment. The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.

Conclusions: Our study provides a novel method of a combination of cryotherapy and anti-fungal agents for treatment of fungal corneal ulcer. This treatment could help facilitate the practice of fungal keratitis treatment in the future.

Show MeSH
Related in: MedlinePlus