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Experimental study on cryotherapy for fungal corneal ulcer.

Chen Y, Yang W, Gao M, Belin MW, Yu H, Yu J - BMC Ophthalmol (2015)

Bottom Line: Different fungi showed different susceptibilities to treatment.The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.This treatment could help facilitate the practice of fungal keratitis treatment in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, General Hospital of Shenyang Military Area Command, No. 83 Wenhua Road, Shenhe District, Shenyang, 110840, China. shuchiwsw@gmail.com.

ABSTRACT

Background: Fungal corneal ulcer is one of the major causes of visual impairment worldwide. Treatment of fungal corneal ulcer mainly depends on anti-fungal agents. In the current study, we developed an integrated combination therapy of cryotherapy and anti-fungal agents to facilitate effective treatment of fungal corneal ulcer.

Methods: Rabbit models of cornea infection were established using a combined method of intrastromal injection and keratoplasty. After treatment with cryotherapy and anti-fungal agents, scanning electron microscopy, transmission electron microscopy, and confocal microscopy were conducted to observe changes in microstructure in the rabbits. Periodic acid Schiff A and hematoxylin and eosin staining were used for detection of histological changes.

Results: Continuous scanning electron microscopy and transmission electron microscopy observations showed that cryothermal treatment inhibited growth of fungal mycelium by destroying fungal cellular structures. Typical cryotherapy was effective in curing fungal corneal ulcer. Different fungi showed different susceptibilities to treatment. The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.

Conclusions: Our study provides a novel method of a combination of cryotherapy and anti-fungal agents for treatment of fungal corneal ulcer. This treatment could help facilitate the practice of fungal keratitis treatment in the future.

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Related in: MedlinePlus

Fungal keratits caused by fungal infection. Different panels represent the results of different observations (slit-lamp, 10% KOH smear, and confocal microscopy) of the three pathogenic fungi.
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Fig1: Fungal keratits caused by fungal infection. Different panels represent the results of different observations (slit-lamp, 10% KOH smear, and confocal microscopy) of the three pathogenic fungi.

Mentions: Among the 77 animals, 63 had successful establishment of the fungal corneal ulcer model (82%). However, 10 rabbits were used for pre-experiment and the data for analyses were derived from the remaining 53 rabbits. Different fungal species showed a significant difference in rate of success in establishment of the model. The success rate of the Candida albicans group was 91.4%, which was similar to that of 82.4% for the Fusarium solani group. However, the success rate of Aspergillus fumigatus was 66.7%. The symptoms of corneal ulcer in the different groups are shown in FigureĀ 1.Figure 1


Experimental study on cryotherapy for fungal corneal ulcer.

Chen Y, Yang W, Gao M, Belin MW, Yu H, Yu J - BMC Ophthalmol (2015)

Fungal keratits caused by fungal infection. Different panels represent the results of different observations (slit-lamp, 10% KOH smear, and confocal microscopy) of the three pathogenic fungi.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4384297&req=5

Fig1: Fungal keratits caused by fungal infection. Different panels represent the results of different observations (slit-lamp, 10% KOH smear, and confocal microscopy) of the three pathogenic fungi.
Mentions: Among the 77 animals, 63 had successful establishment of the fungal corneal ulcer model (82%). However, 10 rabbits were used for pre-experiment and the data for analyses were derived from the remaining 53 rabbits. Different fungal species showed a significant difference in rate of success in establishment of the model. The success rate of the Candida albicans group was 91.4%, which was similar to that of 82.4% for the Fusarium solani group. However, the success rate of Aspergillus fumigatus was 66.7%. The symptoms of corneal ulcer in the different groups are shown in FigureĀ 1.Figure 1

Bottom Line: Different fungi showed different susceptibilities to treatment.The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.This treatment could help facilitate the practice of fungal keratitis treatment in the future.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, General Hospital of Shenyang Military Area Command, No. 83 Wenhua Road, Shenhe District, Shenyang, 110840, China. shuchiwsw@gmail.com.

ABSTRACT

Background: Fungal corneal ulcer is one of the major causes of visual impairment worldwide. Treatment of fungal corneal ulcer mainly depends on anti-fungal agents. In the current study, we developed an integrated combination therapy of cryotherapy and anti-fungal agents to facilitate effective treatment of fungal corneal ulcer.

Methods: Rabbit models of cornea infection were established using a combined method of intrastromal injection and keratoplasty. After treatment with cryotherapy and anti-fungal agents, scanning electron microscopy, transmission electron microscopy, and confocal microscopy were conducted to observe changes in microstructure in the rabbits. Periodic acid Schiff A and hematoxylin and eosin staining were used for detection of histological changes.

Results: Continuous scanning electron microscopy and transmission electron microscopy observations showed that cryothermal treatment inhibited growth of fungal mycelium by destroying fungal cellular structures. Typical cryotherapy was effective in curing fungal corneal ulcer. Different fungi showed different susceptibilities to treatment. The curative effect of Candida albicans was the best, while that of Aspergillus fumigates was the worst.

Conclusions: Our study provides a novel method of a combination of cryotherapy and anti-fungal agents for treatment of fungal corneal ulcer. This treatment could help facilitate the practice of fungal keratitis treatment in the future.

Show MeSH
Related in: MedlinePlus