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Biodegradable chitosan nanoparticle coatings on titanium for the delivery of BMP-2.

Poth N, Seiffart V, Gross G, Menzel H, Dempwolf W - Biomolecules (2015)

Bottom Line: The particle degradation via lysozyme displays a decrease in particle diameter of 40% after 4 days.Drug loaded nanoparticle coatings with biologically active BMP-2 are obtained in vitro within this work.These results show that biodegradable CS-TPP coatings can be utilized to present biologically active BMP-2 on common implant materials like Ti6Al4V.

View Article: PubMed Central - PubMed

Affiliation: Institut für Technische Chemie, Technische Universität Braunschweig, Hans-Sommer-Str. 10, 38106 Braunschweig, Germany. n.poth@tu-bs.de.

ABSTRACT
A simple method for the functionalization of a common implant material (Ti6Al4V) with biodegradable, drug loaded chitosan-tripolyphosphate (CS-TPP) nanoparticles is developed in order to enhance the osseointegration of endoprostheses after revision operations. The chitosan used has a tailored degree of acetylation which allows for a fast biodegradation by lysozyme. The degradability of chitosan is proven via viscometry. Characteristics and degradation of nanoparticles formed with TPP are analyzed using dynamic light scattering. The particle degradation via lysozyme displays a decrease in particle diameter of 40% after 4 days. Drug loading and release is investigated for the nanoparticles with bone morphogenetic protein 2 (BMP-2), using ELISA and the BRE luciferase test for quantification and bioactivity evaluation. Furthermore, nanoparticle coatings on titanium substrates are created via spray-coating and analyzed by ellipsometry, scanning electron microscopy and X-ray photoelectron spectroscopy. Drug loaded nanoparticle coatings with biologically active BMP-2 are obtained in vitro within this work. Additionally, an in vivo study in mice indicates the dose dependent induction of ectopic bone growth through CS-TPP-BMP-2 nanoparticles. These results show that biodegradable CS-TPP coatings can be utilized to present biologically active BMP-2 on common implant materials like Ti6Al4V.

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Related in: MedlinePlus

XPS measurement of a spray-coated chitosan-tripolyphosphate nanoparticle solution film (3:1, 1 mg/mL, 0.075% AcOH, DA 42%) on a titanium substrate and a blank substrate showing the atomic concentrations on the substrates surfaces.
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biomolecules-05-00003-f005: XPS measurement of a spray-coated chitosan-tripolyphosphate nanoparticle solution film (3:1, 1 mg/mL, 0.075% AcOH, DA 42%) on a titanium substrate and a blank substrate showing the atomic concentrations on the substrates surfaces.

Mentions: For further characterization of the coated titanium substrates XPS measurements were carried out, which give insight into the elemental composition of a surface up to a depth of about 10 nm. As shown in Figure 5 for the uncoated surface oxygen and titanium are detected due to the passivizing titanium oxide layer. In addition signals for carbon and nitrogen are found, which are due to common impurities. In comparison, for the CS(42)-TPP nanoparticle coated surface no titanium signal can be detected which indicates a complete coverage of the surface with CS(42)-TPP. Additionally, a signal for phosphor appears and an increase in nitrogen and carbon is obvious. The phosphor can be assigned to the tripolyphosphate (TPP) while carbon, oxygen and nitrogen are present in chitosan. This proves the presence of chitosan and tripolyphosphate on the coated titanium substrates.


Biodegradable chitosan nanoparticle coatings on titanium for the delivery of BMP-2.

Poth N, Seiffart V, Gross G, Menzel H, Dempwolf W - Biomolecules (2015)

XPS measurement of a spray-coated chitosan-tripolyphosphate nanoparticle solution film (3:1, 1 mg/mL, 0.075% AcOH, DA 42%) on a titanium substrate and a blank substrate showing the atomic concentrations on the substrates surfaces.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384108&req=5

biomolecules-05-00003-f005: XPS measurement of a spray-coated chitosan-tripolyphosphate nanoparticle solution film (3:1, 1 mg/mL, 0.075% AcOH, DA 42%) on a titanium substrate and a blank substrate showing the atomic concentrations on the substrates surfaces.
Mentions: For further characterization of the coated titanium substrates XPS measurements were carried out, which give insight into the elemental composition of a surface up to a depth of about 10 nm. As shown in Figure 5 for the uncoated surface oxygen and titanium are detected due to the passivizing titanium oxide layer. In addition signals for carbon and nitrogen are found, which are due to common impurities. In comparison, for the CS(42)-TPP nanoparticle coated surface no titanium signal can be detected which indicates a complete coverage of the surface with CS(42)-TPP. Additionally, a signal for phosphor appears and an increase in nitrogen and carbon is obvious. The phosphor can be assigned to the tripolyphosphate (TPP) while carbon, oxygen and nitrogen are present in chitosan. This proves the presence of chitosan and tripolyphosphate on the coated titanium substrates.

Bottom Line: The particle degradation via lysozyme displays a decrease in particle diameter of 40% after 4 days.Drug loaded nanoparticle coatings with biologically active BMP-2 are obtained in vitro within this work.These results show that biodegradable CS-TPP coatings can be utilized to present biologically active BMP-2 on common implant materials like Ti6Al4V.

View Article: PubMed Central - PubMed

Affiliation: Institut für Technische Chemie, Technische Universität Braunschweig, Hans-Sommer-Str. 10, 38106 Braunschweig, Germany. n.poth@tu-bs.de.

ABSTRACT
A simple method for the functionalization of a common implant material (Ti6Al4V) with biodegradable, drug loaded chitosan-tripolyphosphate (CS-TPP) nanoparticles is developed in order to enhance the osseointegration of endoprostheses after revision operations. The chitosan used has a tailored degree of acetylation which allows for a fast biodegradation by lysozyme. The degradability of chitosan is proven via viscometry. Characteristics and degradation of nanoparticles formed with TPP are analyzed using dynamic light scattering. The particle degradation via lysozyme displays a decrease in particle diameter of 40% after 4 days. Drug loading and release is investigated for the nanoparticles with bone morphogenetic protein 2 (BMP-2), using ELISA and the BRE luciferase test for quantification and bioactivity evaluation. Furthermore, nanoparticle coatings on titanium substrates are created via spray-coating and analyzed by ellipsometry, scanning electron microscopy and X-ray photoelectron spectroscopy. Drug loaded nanoparticle coatings with biologically active BMP-2 are obtained in vitro within this work. Additionally, an in vivo study in mice indicates the dose dependent induction of ectopic bone growth through CS-TPP-BMP-2 nanoparticles. These results show that biodegradable CS-TPP coatings can be utilized to present biologically active BMP-2 on common implant materials like Ti6Al4V.

Show MeSH
Related in: MedlinePlus