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Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation.

Selmin F, Puoci F, Parisi OI, Franzé S, Musazzi UM, Cilurzo F - J Funct Biomater (2015)

Bottom Line: By using a W1/O/W2 technique, g-CA-PLGA improved the encapsulation efficiency (EE), suggesting that the presence of caffeic residues improved the compatibility between components (EEPLGA = 35.0% ± 0.7% vs.The OVA content in g-CA-PLGA microspheres decreased to a lower extent with respect to PLGA microspheres.These results suggest that g-CA-PLGA is a promising biodegradable material to microencapsulate biological drugs.

View Article: PubMed Central - PubMed

Affiliation: Università degli Studi di Milano, Department of Pharmaceutical Sciences-via Giuseppe Colombo, 71-20133 Milano, Italy. francesca.selmin@unimi.it.

ABSTRACT
This work reports the feasibility of caffeic acid grafted PLGA (g-CA-PLGA) to design biodegradable sterile microspheres for the delivery of proteins. Ovalbumin (OVA) was selected as model compound because of its sensitiveness of γ-radiation. The adopted grafting procedure allowed us to obtain a material with good free radical scavenging properties, without a significant modification of Mw and Tg of the starting PLGA (Mw PLGA = 26.3 ± 1.3 kDa vs. Mw g-CA-PLGA = 22.8 ± 0.7 kDa; Tg PLGA = 47.7 ± 0.8 °C vs. Tg g-CA-PLGA = 47.4 ± 0.2 °C). By using a W1/O/W2 technique, g-CA-PLGA improved the encapsulation efficiency (EE), suggesting that the presence of caffeic residues improved the compatibility between components (EEPLGA = 35.0% ± 0.7% vs. EEg-CA-PLGA = 95.6% ± 2.7%). Microspheres particle size distribution ranged from 15 to 50 µm. The zeta-potential values of placebo and loaded microspheres were -25 mV and -15 mV, respectively. The irradiation of g-CA-PLGA at the dose of 25 kGy caused a less than 1% variation of Mw and the degradation patterns of the non-irradiated and irradiated microspheres were superimposable. The OVA content in g-CA-PLGA microspheres decreased to a lower extent with respect to PLGA microspheres. These results suggest that g-CA-PLGA is a promising biodegradable material to microencapsulate biological drugs.

No MeSH data available.


Related in: MedlinePlus

In vitro degradation pattern of g-CA-PLGA microspheres before (black symbol) and after γ-irradiation at the dose of 25 kGy (empty symbol). The results are expressed as the mean of three determinations ± standard deviation.
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jfb-06-00001-f003: In vitro degradation pattern of g-CA-PLGA microspheres before (black symbol) and after γ-irradiation at the dose of 25 kGy (empty symbol). The results are expressed as the mean of three determinations ± standard deviation.

Mentions: Drug release rates from g-CA-PLGA microspheres were improved resulting in more continuous release profiles by contrast to PLGA microspheres. Indeed, the microspheres prepared by using g-CA-PLGA showed a linear relationship between the OVA released fraction and the square root of time indicating that the drug release was controlled only by a diffusion process (R2 = 0.97). Indeed, no significant variations in the polymer Mw occurred up to 14 days (Figure 3). This peculiar pattern might cause also the slight increase in burst effect with respect to that determined in the case of PLGA microspheres.


Caffeic Acid-PLGA Conjugate to Design Protein Drug Delivery Systems Stable to Irradiation.

Selmin F, Puoci F, Parisi OI, Franzé S, Musazzi UM, Cilurzo F - J Funct Biomater (2015)

In vitro degradation pattern of g-CA-PLGA microspheres before (black symbol) and after γ-irradiation at the dose of 25 kGy (empty symbol). The results are expressed as the mean of three determinations ± standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384096&req=5

jfb-06-00001-f003: In vitro degradation pattern of g-CA-PLGA microspheres before (black symbol) and after γ-irradiation at the dose of 25 kGy (empty symbol). The results are expressed as the mean of three determinations ± standard deviation.
Mentions: Drug release rates from g-CA-PLGA microspheres were improved resulting in more continuous release profiles by contrast to PLGA microspheres. Indeed, the microspheres prepared by using g-CA-PLGA showed a linear relationship between the OVA released fraction and the square root of time indicating that the drug release was controlled only by a diffusion process (R2 = 0.97). Indeed, no significant variations in the polymer Mw occurred up to 14 days (Figure 3). This peculiar pattern might cause also the slight increase in burst effect with respect to that determined in the case of PLGA microspheres.

Bottom Line: By using a W1/O/W2 technique, g-CA-PLGA improved the encapsulation efficiency (EE), suggesting that the presence of caffeic residues improved the compatibility between components (EEPLGA = 35.0% ± 0.7% vs.The OVA content in g-CA-PLGA microspheres decreased to a lower extent with respect to PLGA microspheres.These results suggest that g-CA-PLGA is a promising biodegradable material to microencapsulate biological drugs.

View Article: PubMed Central - PubMed

Affiliation: Università degli Studi di Milano, Department of Pharmaceutical Sciences-via Giuseppe Colombo, 71-20133 Milano, Italy. francesca.selmin@unimi.it.

ABSTRACT
This work reports the feasibility of caffeic acid grafted PLGA (g-CA-PLGA) to design biodegradable sterile microspheres for the delivery of proteins. Ovalbumin (OVA) was selected as model compound because of its sensitiveness of γ-radiation. The adopted grafting procedure allowed us to obtain a material with good free radical scavenging properties, without a significant modification of Mw and Tg of the starting PLGA (Mw PLGA = 26.3 ± 1.3 kDa vs. Mw g-CA-PLGA = 22.8 ± 0.7 kDa; Tg PLGA = 47.7 ± 0.8 °C vs. Tg g-CA-PLGA = 47.4 ± 0.2 °C). By using a W1/O/W2 technique, g-CA-PLGA improved the encapsulation efficiency (EE), suggesting that the presence of caffeic residues improved the compatibility between components (EEPLGA = 35.0% ± 0.7% vs. EEg-CA-PLGA = 95.6% ± 2.7%). Microspheres particle size distribution ranged from 15 to 50 µm. The zeta-potential values of placebo and loaded microspheres were -25 mV and -15 mV, respectively. The irradiation of g-CA-PLGA at the dose of 25 kGy caused a less than 1% variation of Mw and the degradation patterns of the non-irradiated and irradiated microspheres were superimposable. The OVA content in g-CA-PLGA microspheres decreased to a lower extent with respect to PLGA microspheres. These results suggest that g-CA-PLGA is a promising biodegradable material to microencapsulate biological drugs.

No MeSH data available.


Related in: MedlinePlus