Limits...
Label-free detection of rare cell in human blood using gold nano slit surface plasmon resonance.

Mousavi MZ, Chen HY, Hou HS, Chang CY, Roffler S, Wei PK, Cheng JY - Biosensors (Basel) (2015)

Bottom Line: The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film.MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area.The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. m.mousavi07@gmail.com.

ABSTRACT
Label-free detection of rare cells in biological samples is an important and highly demanded task for clinical applications and various fields of research, such as detection of circulating tumor cells for cancer therapy and stem cells studies. Surface Plasmon Resonance (SPR) as a label-free method is a promising technology for detection of rare cells for diagnosis or research applications. Short detection depth of SPR (400 nm) provides a sensitive method with minimum interference of non-targets in the biological samples. In this work, we developed a novel microfluidic chip integrated with gold nanoslit SPR platform for highly efficient immunomagnetic capturing and detection of rare cells in human blood. Our method offers simple yet efficient detection of target cells with high purity. The approach for detection consists of two steps. Target cells are firs captured on functionalized magnetic nanoparticles (MNPs) with specific antibody I. The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film. MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area. The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits.

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Related in: MedlinePlus

Identification of captured cells (a), the bright field images of the captured cells (20× magnifications). (b), the fluorescence image of the captured target cells (20× magnifications, exposure time 20 s).
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biosensors-05-00098-f011: Identification of captured cells (a), the bright field images of the captured cells (20× magnifications). (b), the fluorescence image of the captured target cells (20× magnifications, exposure time 20 s).

Mentions: It is important to verify that the cells captured by DCM are the target cells. To identify the captured cells on the nanoslits, the cancer cells were labeled by a fluorescence dye before being spiked into the blood sample. After the red blood cell lysis step, DCM was applied. The image of the captured cells on the gold nanoslits is shown in Figure A4. Bright field images of the captured cells (20× magnifications) are shown in Figure A4a. A fluorescence image of the captured cells is shown in Figure A4b.


Label-free detection of rare cell in human blood using gold nano slit surface plasmon resonance.

Mousavi MZ, Chen HY, Hou HS, Chang CY, Roffler S, Wei PK, Cheng JY - Biosensors (Basel) (2015)

Identification of captured cells (a), the bright field images of the captured cells (20× magnifications). (b), the fluorescence image of the captured target cells (20× magnifications, exposure time 20 s).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384085&req=5

biosensors-05-00098-f011: Identification of captured cells (a), the bright field images of the captured cells (20× magnifications). (b), the fluorescence image of the captured target cells (20× magnifications, exposure time 20 s).
Mentions: It is important to verify that the cells captured by DCM are the target cells. To identify the captured cells on the nanoslits, the cancer cells were labeled by a fluorescence dye before being spiked into the blood sample. After the red blood cell lysis step, DCM was applied. The image of the captured cells on the gold nanoslits is shown in Figure A4. Bright field images of the captured cells (20× magnifications) are shown in Figure A4a. A fluorescence image of the captured cells is shown in Figure A4b.

Bottom Line: The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film.MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area.The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. m.mousavi07@gmail.com.

ABSTRACT
Label-free detection of rare cells in biological samples is an important and highly demanded task for clinical applications and various fields of research, such as detection of circulating tumor cells for cancer therapy and stem cells studies. Surface Plasmon Resonance (SPR) as a label-free method is a promising technology for detection of rare cells for diagnosis or research applications. Short detection depth of SPR (400 nm) provides a sensitive method with minimum interference of non-targets in the biological samples. In this work, we developed a novel microfluidic chip integrated with gold nanoslit SPR platform for highly efficient immunomagnetic capturing and detection of rare cells in human blood. Our method offers simple yet efficient detection of target cells with high purity. The approach for detection consists of two steps. Target cells are firs captured on functionalized magnetic nanoparticles (MNPs) with specific antibody I. The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film. MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area. The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits.

Show MeSH
Related in: MedlinePlus