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Label-free detection of rare cell in human blood using gold nano slit surface plasmon resonance.

Mousavi MZ, Chen HY, Hou HS, Chang CY, Roffler S, Wei PK, Cheng JY - Biosensors (Basel) (2015)

Bottom Line: The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film.MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area.The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. m.mousavi07@gmail.com.

ABSTRACT
Label-free detection of rare cells in biological samples is an important and highly demanded task for clinical applications and various fields of research, such as detection of circulating tumor cells for cancer therapy and stem cells studies. Surface Plasmon Resonance (SPR) as a label-free method is a promising technology for detection of rare cells for diagnosis or research applications. Short detection depth of SPR (400 nm) provides a sensitive method with minimum interference of non-targets in the biological samples. In this work, we developed a novel microfluidic chip integrated with gold nanoslit SPR platform for highly efficient immunomagnetic capturing and detection of rare cells in human blood. Our method offers simple yet efficient detection of target cells with high purity. The approach for detection consists of two steps. Target cells are firs captured on functionalized magnetic nanoparticles (MNPs) with specific antibody I. The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film. MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area. The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits.

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Related in: MedlinePlus

(a) DCM was applied to 1 mL of blood sample spiked with CL1-5 cells. The SPR response at 40 min for the blood sample without the spiked cells and the blood sample spiked with 100 and 1000 cells are shown. (b) The temporal SPR response that indicates the progression of the cell capturing on the gold nanoslits. Sample was introduced to the chip within 30 min. At 30 min, PBS buffer as the washing buffer was introduced. Each data set is the average of two or three measurements. The error bars lengths have been set to either the measurement standard deviation or the spectrometer resolution (±0.4 nm) whichever is larger.
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biosensors-05-00098-f004: (a) DCM was applied to 1 mL of blood sample spiked with CL1-5 cells. The SPR response at 40 min for the blood sample without the spiked cells and the blood sample spiked with 100 and 1000 cells are shown. (b) The temporal SPR response that indicates the progression of the cell capturing on the gold nanoslits. Sample was introduced to the chip within 30 min. At 30 min, PBS buffer as the washing buffer was introduced. Each data set is the average of two or three measurements. The error bars lengths have been set to either the measurement standard deviation or the spectrometer resolution (±0.4 nm) whichever is larger.

Mentions: The result is shown in Figure 4a. At 40 min, a red shift of 0.6 ± 0.4 nm for the blood sample without spiked cells, a shift of 1.7 ± 0.4 nm for the sample spiked with 100 cells and 5.4 ± 0.6 nm shift for the sample with 1000 cells were observed. The corresponding temporal changes of the SPR response is shown in Figure 4b. As it has been shown for the sample of blood only (black dots), at 30 min after introducing the sample, the SPR spectrum was red shifted but the following post-wash step led to a backshift. The backshift after the post-washing step indicates effective elimination of non-specific binding of the blood cells from gold nanoslits. In comparison, the rapid red shift of the SPR spectrum (~5 nm in 20 min) caused by 1000 cells spiked in 1 mL blood confirms the high sensitivity and specificity of our method to detect the target cells in the blood sample.


Label-free detection of rare cell in human blood using gold nano slit surface plasmon resonance.

Mousavi MZ, Chen HY, Hou HS, Chang CY, Roffler S, Wei PK, Cheng JY - Biosensors (Basel) (2015)

(a) DCM was applied to 1 mL of blood sample spiked with CL1-5 cells. The SPR response at 40 min for the blood sample without the spiked cells and the blood sample spiked with 100 and 1000 cells are shown. (b) The temporal SPR response that indicates the progression of the cell capturing on the gold nanoslits. Sample was introduced to the chip within 30 min. At 30 min, PBS buffer as the washing buffer was introduced. Each data set is the average of two or three measurements. The error bars lengths have been set to either the measurement standard deviation or the spectrometer resolution (±0.4 nm) whichever is larger.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384085&req=5

biosensors-05-00098-f004: (a) DCM was applied to 1 mL of blood sample spiked with CL1-5 cells. The SPR response at 40 min for the blood sample without the spiked cells and the blood sample spiked with 100 and 1000 cells are shown. (b) The temporal SPR response that indicates the progression of the cell capturing on the gold nanoslits. Sample was introduced to the chip within 30 min. At 30 min, PBS buffer as the washing buffer was introduced. Each data set is the average of two or three measurements. The error bars lengths have been set to either the measurement standard deviation or the spectrometer resolution (±0.4 nm) whichever is larger.
Mentions: The result is shown in Figure 4a. At 40 min, a red shift of 0.6 ± 0.4 nm for the blood sample without spiked cells, a shift of 1.7 ± 0.4 nm for the sample spiked with 100 cells and 5.4 ± 0.6 nm shift for the sample with 1000 cells were observed. The corresponding temporal changes of the SPR response is shown in Figure 4b. As it has been shown for the sample of blood only (black dots), at 30 min after introducing the sample, the SPR spectrum was red shifted but the following post-wash step led to a backshift. The backshift after the post-washing step indicates effective elimination of non-specific binding of the blood cells from gold nanoslits. In comparison, the rapid red shift of the SPR spectrum (~5 nm in 20 min) caused by 1000 cells spiked in 1 mL blood confirms the high sensitivity and specificity of our method to detect the target cells in the blood sample.

Bottom Line: The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film.MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area.The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits.

View Article: PubMed Central - PubMed

Affiliation: Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan. m.mousavi07@gmail.com.

ABSTRACT
Label-free detection of rare cells in biological samples is an important and highly demanded task for clinical applications and various fields of research, such as detection of circulating tumor cells for cancer therapy and stem cells studies. Surface Plasmon Resonance (SPR) as a label-free method is a promising technology for detection of rare cells for diagnosis or research applications. Short detection depth of SPR (400 nm) provides a sensitive method with minimum interference of non-targets in the biological samples. In this work, we developed a novel microfluidic chip integrated with gold nanoslit SPR platform for highly efficient immunomagnetic capturing and detection of rare cells in human blood. Our method offers simple yet efficient detection of target cells with high purity. The approach for detection consists of two steps. Target cells are firs captured on functionalized magnetic nanoparticles (MNPs) with specific antibody I. The suspension containing the captured cells (MNPs-cells) is then introduced into a microfluidic chip integrated with a gold nanoslit film. MNPs-cells bind with the second specific antibody immobilized on the surface of the gold nanoslit and are therefore captured on the sensor active area. The cell binding on the gold nanoslit was monitored by the wavelength shift of the SPR spectrum generated by the gold nanoslits.

Show MeSH
Related in: MedlinePlus