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Direct determination of a small-molecule drug, valproic Acid, by an electrically-detected microcantilever biosensor for personalized diagnostics.

Huang LS, Gunawan C, Yen YK, Chang KF - Biosensors (Basel) (2015)

Bottom Line: The measured surface stresses showed a profile over a concentration range of 50-500 μg·mL-1, which covered the clinically therapeutic range of 50-100 μg·mL-1.The estimated limit of detection (LOD) was calculated to be 45 μg·mL-1, and the binding affinity between the drug and the antibody was measured at around 90 ± 21 μg·mL-1.Lastly, the results of the proposed device showed a similar profile in valproic acid drug detection with those of the clinically-used fluorescence polarization immunoassay.

View Article: PubMed Central - PubMed

Affiliation: Institute of Applied Mechanics, National Taiwan University, Taipei 10617, Taiwan. lshuang@ntu.edu.tw.

ABSTRACT
Direct, small-molecule determination of the antiepileptic drug, valproic acid, was investigated by a label-free, nanomechanical biosensor. Valproic acid has long been used as an antiepileptic medication, which is administered through therapeutic drug monitoring and has a narrow therapeutic dosage range of 50-100 μg·mL-1 in blood or serum. Unlike labeled and clinically-used measurement techniques, the label-free, electrical detection microcantilever biosensor can be miniaturized and simplified for use in portable or hand-held point-of-care platforms or personal diagnostic tools. A micromachined microcantilever sensor was packaged into the micro-channel of a fluidic system. The measurement of the antiepileptic drug, valproic acid, in phosphate-buffered saline and serum used a single free-standing, piezoresistive microcantilever biosensor in a thermally-controlled system. The measured surface stresses showed a profile over a concentration range of 50-500 μg·mL-1, which covered the clinically therapeutic range of 50-100 μg·mL-1. The estimated limit of detection (LOD) was calculated to be 45 μg·mL-1, and the binding affinity between the drug and the antibody was measured at around 90 ± 21 μg·mL-1. Lastly, the results of the proposed device showed a similar profile in valproic acid drug detection with those of the clinically-used fluorescence polarization immunoassay.

No MeSH data available.


The reproducible, measured steady-state signals of various valproic acid concentrations.
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biosensors-05-00037-f004: The reproducible, measured steady-state signals of various valproic acid concentrations.

Mentions: A series of measurement experiments were conducted to examine the reproducibility of microcantilever detection and to characterize the binding property of drug-antibody interaction. Figure 4 shows a profile of the steady-state response signals as the valproic acid concentration increases. The average steady-state resistance changes with error bars at each concentration obtained for three tests. As the sensor device was treated as being for disposable use for a point-of-care platform or personalized diagnostics, the experiment was designed such that the sensor were not reusable. Hence, each sensor surface was not regenerated in the process, and thus, they were freshly constructed.


Direct determination of a small-molecule drug, valproic Acid, by an electrically-detected microcantilever biosensor for personalized diagnostics.

Huang LS, Gunawan C, Yen YK, Chang KF - Biosensors (Basel) (2015)

The reproducible, measured steady-state signals of various valproic acid concentrations.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384081&req=5

biosensors-05-00037-f004: The reproducible, measured steady-state signals of various valproic acid concentrations.
Mentions: A series of measurement experiments were conducted to examine the reproducibility of microcantilever detection and to characterize the binding property of drug-antibody interaction. Figure 4 shows a profile of the steady-state response signals as the valproic acid concentration increases. The average steady-state resistance changes with error bars at each concentration obtained for three tests. As the sensor device was treated as being for disposable use for a point-of-care platform or personalized diagnostics, the experiment was designed such that the sensor were not reusable. Hence, each sensor surface was not regenerated in the process, and thus, they were freshly constructed.

Bottom Line: The measured surface stresses showed a profile over a concentration range of 50-500 μg·mL-1, which covered the clinically therapeutic range of 50-100 μg·mL-1.The estimated limit of detection (LOD) was calculated to be 45 μg·mL-1, and the binding affinity between the drug and the antibody was measured at around 90 ± 21 μg·mL-1.Lastly, the results of the proposed device showed a similar profile in valproic acid drug detection with those of the clinically-used fluorescence polarization immunoassay.

View Article: PubMed Central - PubMed

Affiliation: Institute of Applied Mechanics, National Taiwan University, Taipei 10617, Taiwan. lshuang@ntu.edu.tw.

ABSTRACT
Direct, small-molecule determination of the antiepileptic drug, valproic acid, was investigated by a label-free, nanomechanical biosensor. Valproic acid has long been used as an antiepileptic medication, which is administered through therapeutic drug monitoring and has a narrow therapeutic dosage range of 50-100 μg·mL-1 in blood or serum. Unlike labeled and clinically-used measurement techniques, the label-free, electrical detection microcantilever biosensor can be miniaturized and simplified for use in portable or hand-held point-of-care platforms or personal diagnostic tools. A micromachined microcantilever sensor was packaged into the micro-channel of a fluidic system. The measurement of the antiepileptic drug, valproic acid, in phosphate-buffered saline and serum used a single free-standing, piezoresistive microcantilever biosensor in a thermally-controlled system. The measured surface stresses showed a profile over a concentration range of 50-500 μg·mL-1, which covered the clinically therapeutic range of 50-100 μg·mL-1. The estimated limit of detection (LOD) was calculated to be 45 μg·mL-1, and the binding affinity between the drug and the antibody was measured at around 90 ± 21 μg·mL-1. Lastly, the results of the proposed device showed a similar profile in valproic acid drug detection with those of the clinically-used fluorescence polarization immunoassay.

No MeSH data available.