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Tracking traction force changes of single cells on the liquid crystal surface.

Soon CF, Tee KS, Youseffi M, Denyer MC - Biosensors (Basel) (2015)

Bottom Line: In this study, time-lapse photo microscopy allowed cell induced deformations in liquid crystal coated substrates to be monitored and analyzed.The results indicated that the system could be used to monitor the generation of cell/surface forces in an initially quiescent cell, as it migrated over the culture substrate, via multiple points of contact between the cell and the surface.Future application of this system is the real-time assaying of the pharmacological effects of cytokines on the mechanics of cell migration.

View Article: PubMed Central - PubMed

Affiliation: Biosensor and Bioengineering Laboratory, MiNT-SRC, Universiti Tun Hussein Onn Malaysia, 83000 Batu Pahat, Malaysia. soon@uthm.edu.my.

ABSTRACT
Cell migration is a key contributor to wound repair. This study presents findings indicating that the liquid crystal based cell traction force transducer (LCTFT) system can be used in conjunction with a bespoke cell traction force mapping (CTFM) software to monitor cell/surface traction forces from quiescent state in real time. In this study, time-lapse photo microscopy allowed cell induced deformations in liquid crystal coated substrates to be monitored and analyzed. The results indicated that the system could be used to monitor the generation of cell/surface forces in an initially quiescent cell, as it migrated over the culture substrate, via multiple points of contact between the cell and the surface. Future application of this system is the real-time assaying of the pharmacological effects of cytokines on the mechanics of cell migration.

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Related in: MedlinePlus

Time-base tractions of a keratinocyte on a liquid crystal based cell traction force transducer (LCTFT) displayed in (a) phase contrast micrographs which were taken at 0, 5, 10 and 15 min of monitoring. The broken line arrow indicates the direction of movement and the dotted lines are the position of reference for the cell; (b) The associated distribution of traction forces; (c) The directions of forces as shown with thick arrows and the thin arrows represent the direction of the forces and actin bundles flow, respectively. The scale bar in pseudo color represents the magnitude of forces in nano-newton. (Scale bar: 20 µm).
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biosensors-05-00013-f001: Time-base tractions of a keratinocyte on a liquid crystal based cell traction force transducer (LCTFT) displayed in (a) phase contrast micrographs which were taken at 0, 5, 10 and 15 min of monitoring. The broken line arrow indicates the direction of movement and the dotted lines are the position of reference for the cell; (b) The associated distribution of traction forces; (c) The directions of forces as shown with thick arrows and the thin arrows represent the direction of the forces and actin bundles flow, respectively. The scale bar in pseudo color represents the magnitude of forces in nano-newton. (Scale bar: 20 µm).

Mentions: Figure 1 shows the time-lapse photo microscopy of a cell attached and migrated across the LC surface. As the cell migrate, cell traction forces translated into deformations that transiently rose and decayed over the LC surface. These deformations were quantified and rendered as a force distribution map according to the method reported in our previous work [16]. As shown in Figure 1a, cell area was divided into four regimes that are the trailing edge (rear), the leading edge (front) and the two lateral flanks (margins).


Tracking traction force changes of single cells on the liquid crystal surface.

Soon CF, Tee KS, Youseffi M, Denyer MC - Biosensors (Basel) (2015)

Time-base tractions of a keratinocyte on a liquid crystal based cell traction force transducer (LCTFT) displayed in (a) phase contrast micrographs which were taken at 0, 5, 10 and 15 min of monitoring. The broken line arrow indicates the direction of movement and the dotted lines are the position of reference for the cell; (b) The associated distribution of traction forces; (c) The directions of forces as shown with thick arrows and the thin arrows represent the direction of the forces and actin bundles flow, respectively. The scale bar in pseudo color represents the magnitude of forces in nano-newton. (Scale bar: 20 µm).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4384078&req=5

biosensors-05-00013-f001: Time-base tractions of a keratinocyte on a liquid crystal based cell traction force transducer (LCTFT) displayed in (a) phase contrast micrographs which were taken at 0, 5, 10 and 15 min of monitoring. The broken line arrow indicates the direction of movement and the dotted lines are the position of reference for the cell; (b) The associated distribution of traction forces; (c) The directions of forces as shown with thick arrows and the thin arrows represent the direction of the forces and actin bundles flow, respectively. The scale bar in pseudo color represents the magnitude of forces in nano-newton. (Scale bar: 20 µm).
Mentions: Figure 1 shows the time-lapse photo microscopy of a cell attached and migrated across the LC surface. As the cell migrate, cell traction forces translated into deformations that transiently rose and decayed over the LC surface. These deformations were quantified and rendered as a force distribution map according to the method reported in our previous work [16]. As shown in Figure 1a, cell area was divided into four regimes that are the trailing edge (rear), the leading edge (front) and the two lateral flanks (margins).

Bottom Line: In this study, time-lapse photo microscopy allowed cell induced deformations in liquid crystal coated substrates to be monitored and analyzed.The results indicated that the system could be used to monitor the generation of cell/surface forces in an initially quiescent cell, as it migrated over the culture substrate, via multiple points of contact between the cell and the surface.Future application of this system is the real-time assaying of the pharmacological effects of cytokines on the mechanics of cell migration.

View Article: PubMed Central - PubMed

Affiliation: Biosensor and Bioengineering Laboratory, MiNT-SRC, Universiti Tun Hussein Onn Malaysia, 83000 Batu Pahat, Malaysia. soon@uthm.edu.my.

ABSTRACT
Cell migration is a key contributor to wound repair. This study presents findings indicating that the liquid crystal based cell traction force transducer (LCTFT) system can be used in conjunction with a bespoke cell traction force mapping (CTFM) software to monitor cell/surface traction forces from quiescent state in real time. In this study, time-lapse photo microscopy allowed cell induced deformations in liquid crystal coated substrates to be monitored and analyzed. The results indicated that the system could be used to monitor the generation of cell/surface forces in an initially quiescent cell, as it migrated over the culture substrate, via multiple points of contact between the cell and the surface. Future application of this system is the real-time assaying of the pharmacological effects of cytokines on the mechanics of cell migration.

Show MeSH
Related in: MedlinePlus