The Addgene repository: an international nonprofit plasmid and data resource.
Bottom Line: The library includes a large number of plasmid tools for use in a wide variety of research areas, such as empty backbones, lentiviral resources, fluorescent protein vectors and genome engineering tools.The Addgene Repository database is always evolving with new plasmid deposits so it contains currently pertinent resources while ensuring the information on earlier deposits is still available.Extensive educational materials and information are provided by the database curators to support the scientists that are accessing the repository's materials and data.
Affiliation: Addgene, Cambridge, MA 02139, USA email@example.com.Show MeSH
Mentions: Each plasmid in the collection has a unique Addgene Accession number and Plasmid Data Page (Figure 3). The Plasmid Data Page first displays the depositing laboratory and the associated publication, if applicable. The ‘Purpose’ field information is provided by depositors or by Addgene Repository curators. It describes, in simple terms, a suggested experimental use for the plasmid. This field can be particularly helpful when scientists are comparing similar reagents to decide on what will work best for their planned experiments. The Plasmid Data Page displays details of the backbone, growth conditions, gene inserts and cloning information for each inserted sequence. Any additional documents that have been provided by the depositing scientist, such as protocols or maps, can be found under ‘Resource Information’. Maps for the Plasmid Data Page are constructed based on available sequence information. Some sequences might be partial or insert-only sequence. Actual or predicted full sequence is available for some plasmids. From the ‘View all sequences’ link on the Plasmid Data page, users can access a simple analysis tool for basic sequence manipulations including feature mapping, sequence download, NCBI ‘BLAST’ search, sequence alignment, restriction pattern prediction and translation.
Affiliation: Addgene, Cambridge, MA 02139, USA firstname.lastname@example.org.