The Addgene repository: an international nonprofit plasmid and data resource.
Bottom Line: The library includes a large number of plasmid tools for use in a wide variety of research areas, such as empty backbones, lentiviral resources, fluorescent protein vectors and genome engineering tools.The Addgene Repository database is always evolving with new plasmid deposits so it contains currently pertinent resources while ensuring the information on earlier deposits is still available.Extensive educational materials and information are provided by the database curators to support the scientists that are accessing the repository's materials and data.
Affiliation: Addgene, Cambridge, MA 02139, USA firstname.lastname@example.org.Show MeSH
Mentions: Plasmids in the database are useful for studies in a wide variety of different experimental systems and model organisms. This diversity reflects the research fields of the >2000 depositing laboratories from >500 institutions around the world. Almost half of the library's plasmids have been designated as containing human gene sequence inserts (Figure 1). Most fields of research are represented in the library. Some areas for which the repository has especially rich resources include: neuroscience, stem cell research, lentiviral transduction, fluorescent protein tagging, optogenetics and genome engineering. A review of the 25 most accessed plasmids over time reflects popular plasmid technologies that are all well represented in the library (12). This top 25 list includes plasmids for retroviral expression, lentiviral expression, luciferase reporter assays, RNA interference studies, generation of induced pluripotent stem cells, mammalian Cre-Lox control and TALEN and CRISPR plasmids for genome engineering.
Affiliation: Addgene, Cambridge, MA 02139, USA email@example.com.