EHFPI: a database and analysis resource of essential host factors for pathogenic infection.
Bottom Line: High-throughput screening and computational technology has greatly changed the face of microbiology in better understanding pathogen-host interactions.The EHFPI web interface provides appropriate tools that allow efficient query of EHF data and visualization of custom-made analysis results.EHFPI data and tools shall keep available without charge and serve the microbiology, biomedicine and pharmaceutics research communities, to finally facilitate the development of diagnostics, prophylactics and therapeutics for human pathogens.
Affiliation: Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing 100850, P.R.China.Show MeSH
Related in: MedlinePlus
Mentions: For the current use case, the PIP analysis identified 260 out of 1213 EHF genes (21.43%) for IAV as PIPs (Figure 4A), which means the large proportion of EHF gene products (78.57%) are not direct interacting proteins for IAV. Meanwhile, a total of 876 interactions were observed for the 260 EHF gene products that are also PIPs for IAV. Of note, the most highly interconnected EHF gene products for IAV include TP53 (i.e. 272 interactions, Figure 4B), EP300 (i.e. 211 interactions), GRB2 (i.e. 195 interactions) and PRKCA (i.e. 174 interactions), these basic topological properties of which can be easily summarized in the csv file for user download (Supplementary Table S2). This result implies that dysregulation of these EHF genes upon infection may perturb multiple host cell functions of biological essentiality and thereby affect the consequences where infection may lead to. Further, users can refer to the Search result on the start page of PIP analysis for detailed PIP resource information on interested EHF(s), e.g. TP53 (cellular tumor antigen p53, Figure 4A). This highly interacted EHF gene product of IAV, is also the PIP for eight other virus types, including HIV-1, HCV, Simian virus 40, Human adenovirus 1 and 2, Human papillomavirus (type 11, 16, 18 and 31), Bovine papillomavirus type 1, Alphapapillomavirus 7 and JC polyomavirus. Together, this comparative analysis of EHF and PIP provides insights into the functional understanding of EHFs at translational level.
Affiliation: Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing 100850, P.R.China.