DBTMEE: a database of transcriptome in mouse early embryos.
Bottom Line: Thereby, users can extensively investigate molecular characteristics among totipotent, pluripotent and differentiated cells while taking genetic and epigenetic characteristics into consideration.We have also designed user friendly web interfaces that enable users to access the data quickly and easily.DBTMEE will help to promote our understanding of the enigmatic fertilization dynamics.
Affiliation: Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.Show MeSH
Mentions: DBTMEE provides a searchable gene expression profile during normal and parthenogenetic early embryo developments that were established previously (6). In brief, we extracted high-quality metaphase II oocytes (Oo) and one-cell stage (1C), two-cell (2C) and four-cell (4C) stage embryos by in vitro fertilization. In addition, we collected mouse embryonic fibroblast cells (MEFs) and parthenogenetic 1C (p1C) and 4C (p4C) embryos. Total RNAs from ≥104 × 2 cells per stage were sequenced by SOLiD system and analyzed by Bioinformatics tools, detecting 17 486 genes that exhibited intriguing expression patterns (Figures 1 and 2). The gene expression patterns categorized by hierarchical clustering are browsable from the front page of DBTMEE (Figure 3A-4).
Affiliation: Human Genome Center, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.