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Clinicopathologic features of 112 cases with mantle cell lymphoma.

Zhou DM, Chen G, Zheng XW, Zhu WF, Chen BZ - Cancer Biol Med (2015)

Bottom Line: A break in the CCND1 gene was not found in 3 cases with CD5-MCL.The prognoses of blastoid and polymorphic subtypes are poor.Special subtypes should be classified during diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Key Laboratory of Cancer Translational Medicine of Fujian, Teaching Hospital of Fujian Medical University, Fujian Tumor Hospital, Fuzhou 350014, China.

ABSTRACT

Objective: This study aims to explore the clinicopathologic features of 112 patients with mantle cell lymphoma (MCL).

Methods: Data from 112 MCL cases were collected, and immunohistochemical assay was conducted. Fluorescence in situ hybridization (FISH) detected a break in the CCND1 gene. The t-test was used in the statistical analysis.

Results: All tumor cells in the 112 cases expressed B cell-related antigen, including 1 blastoid subtype and 1 polymorphic subtype. Among all cases, 106 expressed CD5 and 104 expressed cyclin D1. A break in the CCND1 gene was not found in 3 cases with CD5-MCL. IgH/CCND1 polyploid was observed in 2 classic cases.

Conclusion: MCL is a type of special immunophenotypic B-cell lymphoma. The prognoses of blastoid and polymorphic subtypes are poor. Special subtypes should be classified during diagnosis.

No MeSH data available.


Related in: MedlinePlus

Polymorphic variant MCL. (A) Diffused tumor cells and polymorphic subtype with apparent nucleolus and high mitotic rate (H&E, ×400); (B) Immunohistochemistry: cyclin D1 (+) (Envision, ×400); (C) CD5 (+) (Envision, ×400). MCL, mantle cell lymphoma.
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f2: Polymorphic variant MCL. (A) Diffused tumor cells and polymorphic subtype with apparent nucleolus and high mitotic rate (H&E, ×400); (B) Immunohistochemistry: cyclin D1 (+) (Envision, ×400); (C) CD5 (+) (Envision, ×400). MCL, mantle cell lymphoma.

Mentions: The 112 cases of MCL can be divided into mantle zone type (Figure 1), namely, nodular and diffuse, according to organizational structure. They can also be divided into classic and variant types (such as blastoid variant and polymorphic variant) according to cytologic morphology. The proliferation of single lymphoid cell was visible and formed by a majority of small- to medium-sized cells. The nucleus showed a slightly irregular contour and was similar to the center cell, whereas the nucleolus was not evident. The tumors lack transformed cells (centroblast) or immunoblast cells; no proliferation center was observed. Hyaline degeneration of small vessels, which scattered in the absence of phagocytosis of epithelioid histiocytes, could be observed in some cases. One case of pleomorphic variant of MCL’s tumor cells showed a medium or large size and was diffused, with pleomorphic nuclei. Nuclei were mostly rounding, but some were irregular, distorted, and recessed, with dispersed chromatin. Nucleoli were visible, and high nuclear division rate was observed (Figure 2). One case of blastoid variant of MCL’s tumor cells was similar to lymphoblasts. It exhibited a medium size, and its nuclei were round, with sparse chromatin. Nucleolus was not evident, and high nuclear division rate was observed (Figure 3).


Clinicopathologic features of 112 cases with mantle cell lymphoma.

Zhou DM, Chen G, Zheng XW, Zhu WF, Chen BZ - Cancer Biol Med (2015)

Polymorphic variant MCL. (A) Diffused tumor cells and polymorphic subtype with apparent nucleolus and high mitotic rate (H&E, ×400); (B) Immunohistochemistry: cyclin D1 (+) (Envision, ×400); (C) CD5 (+) (Envision, ×400). MCL, mantle cell lymphoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383843&req=5

f2: Polymorphic variant MCL. (A) Diffused tumor cells and polymorphic subtype with apparent nucleolus and high mitotic rate (H&E, ×400); (B) Immunohistochemistry: cyclin D1 (+) (Envision, ×400); (C) CD5 (+) (Envision, ×400). MCL, mantle cell lymphoma.
Mentions: The 112 cases of MCL can be divided into mantle zone type (Figure 1), namely, nodular and diffuse, according to organizational structure. They can also be divided into classic and variant types (such as blastoid variant and polymorphic variant) according to cytologic morphology. The proliferation of single lymphoid cell was visible and formed by a majority of small- to medium-sized cells. The nucleus showed a slightly irregular contour and was similar to the center cell, whereas the nucleolus was not evident. The tumors lack transformed cells (centroblast) or immunoblast cells; no proliferation center was observed. Hyaline degeneration of small vessels, which scattered in the absence of phagocytosis of epithelioid histiocytes, could be observed in some cases. One case of pleomorphic variant of MCL’s tumor cells showed a medium or large size and was diffused, with pleomorphic nuclei. Nuclei were mostly rounding, but some were irregular, distorted, and recessed, with dispersed chromatin. Nucleoli were visible, and high nuclear division rate was observed (Figure 2). One case of blastoid variant of MCL’s tumor cells was similar to lymphoblasts. It exhibited a medium size, and its nuclei were round, with sparse chromatin. Nucleolus was not evident, and high nuclear division rate was observed (Figure 3).

Bottom Line: A break in the CCND1 gene was not found in 3 cases with CD5-MCL.The prognoses of blastoid and polymorphic subtypes are poor.Special subtypes should be classified during diagnosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Key Laboratory of Cancer Translational Medicine of Fujian, Teaching Hospital of Fujian Medical University, Fujian Tumor Hospital, Fuzhou 350014, China.

ABSTRACT

Objective: This study aims to explore the clinicopathologic features of 112 patients with mantle cell lymphoma (MCL).

Methods: Data from 112 MCL cases were collected, and immunohistochemical assay was conducted. Fluorescence in situ hybridization (FISH) detected a break in the CCND1 gene. The t-test was used in the statistical analysis.

Results: All tumor cells in the 112 cases expressed B cell-related antigen, including 1 blastoid subtype and 1 polymorphic subtype. Among all cases, 106 expressed CD5 and 104 expressed cyclin D1. A break in the CCND1 gene was not found in 3 cases with CD5-MCL. IgH/CCND1 polyploid was observed in 2 classic cases.

Conclusion: MCL is a type of special immunophenotypic B-cell lymphoma. The prognoses of blastoid and polymorphic subtypes are poor. Special subtypes should be classified during diagnosis.

No MeSH data available.


Related in: MedlinePlus