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PAHs in baby food: assessment of three different processing techniques for the preparation of reference materials.

Huertas-Pérez JF, Bordajandi LR, Sejerøe-Olsen B, Emteborg H, Baù A, Schimmel H, Dabrio M - Anal Bioanal Chem (2015)

Bottom Line: To this end, an analytical method based on a solid-liquid extraction followed by cleaning up with gel permeation chromatography (GPC) and solid phase extraction (SPE) and GC-IDMS determination, was validated in-house.It could be demonstrated that the procedure fulfilled the demands for application to the homogeneity and isochronous stability studies for the candidate reference materials targeted here.All three materials proved to be sufficiently homogeneous for the intended use.

View Article: PubMed Central - PubMed

Affiliation: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, 2440, Geel, Belgium, huertas@ugr.es.

ABSTRACT
A feasibility study for producing a matrix reference material for selected polycyclic aromatic hydrocarbons (PAHs) in baby food is reported. A commercially available baby food, containing carrots, potatoes, tomato, white beans and meat, was spiked with the so-called 15 + 1 PAHs included in the PAHs priority list for food of the EU, at a mass fraction level of 1 μg/kg. The contaminated baby food was further processed by autoclaving, freezing or freeze drying. The homogeneity of the three materials (bottle-to-bottle variation) and their short-term (4 weeks) and long-term (18 months) stability at different temperatures were assessed. To this end, an analytical method based on a solid-liquid extraction followed by cleaning up with gel permeation chromatography (GPC) and solid phase extraction (SPE) and GC-IDMS determination, was validated in-house. It could be demonstrated that the procedure fulfilled the demands for application to the homogeneity and isochronous stability studies for the candidate reference materials targeted here. All three materials proved to be sufficiently homogeneous for the intended use. Measurements on the autoclaved material provided the most promising results in terms of envisaged shelf life, although freeze drying was also found to be a suitable processing technique for most of the investigated PAHs. These results are an important step towards the development of a matrix reference material for PAHs in a processed food matrix in a presentation very similar to routine samples.

No MeSH data available.


Related in: MedlinePlus

GC-MS chromatogram corresponding to frozen, autoclaved and freeze-dried materials. Identification of peaks: 1, BcF; 2, BaA; 3, Chr; 4, CPP; 5, 5-MC; 6, BbF; 7, BkF; 8, BjF; 9, BaP; 10, IcdP; 11, DahA; 12, BghiP; 13, DalP; 14, DaeP; 15, DaiP; 16, DahP
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Fig2: GC-MS chromatogram corresponding to frozen, autoclaved and freeze-dried materials. Identification of peaks: 1, BcF; 2, BaA; 3, Chr; 4, CPP; 5, 5-MC; 6, BbF; 7, BkF; 8, BjF; 9, BaP; 10, IcdP; 11, DahA; 12, BghiP; 13, DalP; 14, DaeP; 15, DaiP; 16, DahP

Mentions: It was concluded during the development of the method that optimal results are obtained by the use of a DB-17HT column. This column provided sufficient separation of the target analytes. Closely eluting peaks such as CPP/Chr, BbF/BkF and IcdP/DahA were separated by a valley equal or minor to 20 % of the height of the smallest peak within the pair. The analytes were identified and confirmed by the following criteria: relative retention time (tR in the sample/tR in calibration solution) ≥0.99, presence of the quantification ion as well as one confirmation ion (Table 2) and relative qualifying ion abundance in sample within ±15 % of the analogous compound in the calibration solution. The criteria were met for all analytes down to a level comparable with the estimated LODs, except for 5-MC, for which an interference was observed, possibly because of the presence and co-elution of another MC isomer. Thus, the validation criterion regarding selectivity was not entirely fulfilled for this compound, and therefore it was not considered for subsequent studies. Figure 2 shows typical chromatograms from processed FR, AC and FD samples.Fig. 2


PAHs in baby food: assessment of three different processing techniques for the preparation of reference materials.

Huertas-Pérez JF, Bordajandi LR, Sejerøe-Olsen B, Emteborg H, Baù A, Schimmel H, Dabrio M - Anal Bioanal Chem (2015)

GC-MS chromatogram corresponding to frozen, autoclaved and freeze-dried materials. Identification of peaks: 1, BcF; 2, BaA; 3, Chr; 4, CPP; 5, 5-MC; 6, BbF; 7, BkF; 8, BjF; 9, BaP; 10, IcdP; 11, DahA; 12, BghiP; 13, DalP; 14, DaeP; 15, DaiP; 16, DahP
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4383830&req=5

Fig2: GC-MS chromatogram corresponding to frozen, autoclaved and freeze-dried materials. Identification of peaks: 1, BcF; 2, BaA; 3, Chr; 4, CPP; 5, 5-MC; 6, BbF; 7, BkF; 8, BjF; 9, BaP; 10, IcdP; 11, DahA; 12, BghiP; 13, DalP; 14, DaeP; 15, DaiP; 16, DahP
Mentions: It was concluded during the development of the method that optimal results are obtained by the use of a DB-17HT column. This column provided sufficient separation of the target analytes. Closely eluting peaks such as CPP/Chr, BbF/BkF and IcdP/DahA were separated by a valley equal or minor to 20 % of the height of the smallest peak within the pair. The analytes were identified and confirmed by the following criteria: relative retention time (tR in the sample/tR in calibration solution) ≥0.99, presence of the quantification ion as well as one confirmation ion (Table 2) and relative qualifying ion abundance in sample within ±15 % of the analogous compound in the calibration solution. The criteria were met for all analytes down to a level comparable with the estimated LODs, except for 5-MC, for which an interference was observed, possibly because of the presence and co-elution of another MC isomer. Thus, the validation criterion regarding selectivity was not entirely fulfilled for this compound, and therefore it was not considered for subsequent studies. Figure 2 shows typical chromatograms from processed FR, AC and FD samples.Fig. 2

Bottom Line: To this end, an analytical method based on a solid-liquid extraction followed by cleaning up with gel permeation chromatography (GPC) and solid phase extraction (SPE) and GC-IDMS determination, was validated in-house.It could be demonstrated that the procedure fulfilled the demands for application to the homogeneity and isochronous stability studies for the candidate reference materials targeted here.All three materials proved to be sufficiently homogeneous for the intended use.

View Article: PubMed Central - PubMed

Affiliation: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Retieseweg 111, 2440, Geel, Belgium, huertas@ugr.es.

ABSTRACT
A feasibility study for producing a matrix reference material for selected polycyclic aromatic hydrocarbons (PAHs) in baby food is reported. A commercially available baby food, containing carrots, potatoes, tomato, white beans and meat, was spiked with the so-called 15 + 1 PAHs included in the PAHs priority list for food of the EU, at a mass fraction level of 1 μg/kg. The contaminated baby food was further processed by autoclaving, freezing or freeze drying. The homogeneity of the three materials (bottle-to-bottle variation) and their short-term (4 weeks) and long-term (18 months) stability at different temperatures were assessed. To this end, an analytical method based on a solid-liquid extraction followed by cleaning up with gel permeation chromatography (GPC) and solid phase extraction (SPE) and GC-IDMS determination, was validated in-house. It could be demonstrated that the procedure fulfilled the demands for application to the homogeneity and isochronous stability studies for the candidate reference materials targeted here. All three materials proved to be sufficiently homogeneous for the intended use. Measurements on the autoclaved material provided the most promising results in terms of envisaged shelf life, although freeze drying was also found to be a suitable processing technique for most of the investigated PAHs. These results are an important step towards the development of a matrix reference material for PAHs in a processed food matrix in a presentation very similar to routine samples.

No MeSH data available.


Related in: MedlinePlus