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Surveillance of avian paramyxovirus serotype-1 in migratory waterfowls in Japan between 2011 and 2013.

Mase M, Kanehira K - J. Vet. Med. Sci. (2014)

Bottom Line: Phylogenetic analysis based on the partial fusion protein gene classified these APMV-1 isolates into 2 major genetic groups.Four isolates were classified as class II genotype I, and they were genetically closely related to strains isolated in Asian countries, including Japan.In contrast, two isolates were classified as class I, and they were genetically closely related to strains mainly isolated in the U.S.A.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Animal Health, 3-1-5 Kannondai, Ibaraki 305-0856; United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido 501-1193, Japan. masema@affrc.go.jp

ABSTRACT
To further understand the epidemiology of avian paramyxovirus serotype-1 (APMV-1) in migratory waterfowls in Japan, we conducted the surveillance of this virus from feces derived from the migratory waterfowls collected in 41 Japanese prefectures between October 2011 and March 2013. Six APMV-1 viruses were isolated from total 661 samples. All isolates were identified as the avirulent (lentogenic) type on the basis of intracerebral pathogenicity tests. Genetic analysis showed that these viruses possessed the deduced amino acid sequence of 112GKQGR-L117 or 112ERQER-L117 at the cleavage site of the F0 protein, which was identical to the motif in the avirulent type. Phylogenetic analysis based on the partial fusion protein gene classified these APMV-1 isolates into 2 major genetic groups. Four isolates were classified as class II genotype I, and they were genetically closely related to strains isolated in Asian countries, including Japan. In contrast, two isolates were classified as class I, and they were genetically closely related to strains mainly isolated in the U.S.A.

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Related in: MedlinePlus

Phylogenetic tree of the APMV-1 isolates based on nucleotide sequences from a portion(nt 47- 420) of the F gene. The horizontal distances are proportional to the minimumnumber of nucleotide differences required to join nodes and sequences. The isolatesdescribed in this study are shown by black circles. The tree was generated using theneighbor-joining algorithm, and alignments were bootstrapped 1,000 times. Bootstrapvalues >70 are shown.
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fig_001: Phylogenetic tree of the APMV-1 isolates based on nucleotide sequences from a portion(nt 47- 420) of the F gene. The horizontal distances are proportional to the minimumnumber of nucleotide differences required to join nodes and sequences. The isolatesdescribed in this study are shown by black circles. The tree was generated using theneighbor-joining algorithm, and alignments were bootstrapped 1,000 times. Bootstrapvalues >70 are shown.

Mentions: Phylogenetic analysis based on the partial fusion protein gene classified these APMV-1isolates into 2 major genetic groups (Fig. 1Fig. 1.


Surveillance of avian paramyxovirus serotype-1 in migratory waterfowls in Japan between 2011 and 2013.

Mase M, Kanehira K - J. Vet. Med. Sci. (2014)

Phylogenetic tree of the APMV-1 isolates based on nucleotide sequences from a portion(nt 47- 420) of the F gene. The horizontal distances are proportional to the minimumnumber of nucleotide differences required to join nodes and sequences. The isolatesdescribed in this study are shown by black circles. The tree was generated using theneighbor-joining algorithm, and alignments were bootstrapped 1,000 times. Bootstrapvalues >70 are shown.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383790&req=5

fig_001: Phylogenetic tree of the APMV-1 isolates based on nucleotide sequences from a portion(nt 47- 420) of the F gene. The horizontal distances are proportional to the minimumnumber of nucleotide differences required to join nodes and sequences. The isolatesdescribed in this study are shown by black circles. The tree was generated using theneighbor-joining algorithm, and alignments were bootstrapped 1,000 times. Bootstrapvalues >70 are shown.
Mentions: Phylogenetic analysis based on the partial fusion protein gene classified these APMV-1isolates into 2 major genetic groups (Fig. 1Fig. 1.

Bottom Line: Phylogenetic analysis based on the partial fusion protein gene classified these APMV-1 isolates into 2 major genetic groups.Four isolates were classified as class II genotype I, and they were genetically closely related to strains isolated in Asian countries, including Japan.In contrast, two isolates were classified as class I, and they were genetically closely related to strains mainly isolated in the U.S.A.

View Article: PubMed Central - PubMed

Affiliation: National Institute of Animal Health, 3-1-5 Kannondai, Ibaraki 305-0856; United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido 501-1193, Japan. masema@affrc.go.jp

ABSTRACT
To further understand the epidemiology of avian paramyxovirus serotype-1 (APMV-1) in migratory waterfowls in Japan, we conducted the surveillance of this virus from feces derived from the migratory waterfowls collected in 41 Japanese prefectures between October 2011 and March 2013. Six APMV-1 viruses were isolated from total 661 samples. All isolates were identified as the avirulent (lentogenic) type on the basis of intracerebral pathogenicity tests. Genetic analysis showed that these viruses possessed the deduced amino acid sequence of 112GKQGR-L117 or 112ERQER-L117 at the cleavage site of the F0 protein, which was identical to the motif in the avirulent type. Phylogenetic analysis based on the partial fusion protein gene classified these APMV-1 isolates into 2 major genetic groups. Four isolates were classified as class II genotype I, and they were genetically closely related to strains isolated in Asian countries, including Japan. In contrast, two isolates were classified as class I, and they were genetically closely related to strains mainly isolated in the U.S.A.

Show MeSH
Related in: MedlinePlus