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Molecular characterization of an influenza A virus (H4N2) isolated from waterfowl habitats in the State of Mexico.

Ornelas-Eusebio E, Obregón-Ascencio A, Chávez-Maya F, García-Espinosa G - J. Vet. Med. Sci. (2014)

Bottom Line: This study describes the molecular characterization of an IAV isolated from 240 water samples of a small wetland during non-breeding season of migratory wild ducks in the State of Mexico, Mexico.The results showed that the virus belongs to the H4N2 subtype and each of its eight segments of the viral genome has similarity to IAV isolated from ducks in North America.This study suggests that IAV can be isolated from small wetland during non-breeding season of migrating waterfowl.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000 CP 04510, D.F., México.

ABSTRACT
Wild waterfowl and their habitats are the main reservoirs of influenza A virus (IAV) mainly during the breeding season and prior to migration. This study describes the molecular characterization of an IAV isolated from 240 water samples of a small wetland during non-breeding season of migratory wild ducks in the State of Mexico, Mexico. The results showed that the virus belongs to the H4N2 subtype and each of its eight segments of the viral genome has similarity to IAV isolated from ducks in North America. This study suggests that IAV can be isolated from small wetland during non-breeding season of migrating waterfowl.

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Related in: MedlinePlus

Phylogenetic tree of the HA gene of A/environment/Mexico/CPA-04740/2011 (H4N2) [■]Influenza A virus isolated from a wetland. The phylogenetic tree was constructed withthe Neighbor-Joining method. The percentages of replicate trees in which the associatedtaxa clustered together in a bootstrap test (1,000 replicates) are shown next to thebranches. The evolutionary distances were computed using the Kimura 2-parameter method.The analysis involved 45 nucleotide sequences. Evolutionary analyses were conducted inMEGA5.05.
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fig_001: Phylogenetic tree of the HA gene of A/environment/Mexico/CPA-04740/2011 (H4N2) [■]Influenza A virus isolated from a wetland. The phylogenetic tree was constructed withthe Neighbor-Joining method. The percentages of replicate trees in which the associatedtaxa clustered together in a bootstrap test (1,000 replicates) are shown next to thebranches. The evolutionary distances were computed using the Kimura 2-parameter method.The analysis involved 45 nucleotide sequences. Evolutionary analyses were conducted inMEGA5.05.

Mentions: We found that HA gene of the virus showed 99% similarity to the homologous sequence of thevirus A/blue-winged teal/LA/AI13-1334/2013 (H4N2) [KJ413565.1] and 98% similarity to theviruses A/pekin duck/California/P30/2006 (H4N2) [CY053821.1],A/environment/California/NWRC183970-18/2006 (H4N2) [CY122499.1] and A/environment/NewMexico/NWRC184137-06/2006 (H4N2) [CY122503.1], except for other isolates of the H4 subtypefrom North American wild waterfowl and environmental samples, collected between 1975 and 2009with similarity <97% (Fig. 1Fig. 1.


Molecular characterization of an influenza A virus (H4N2) isolated from waterfowl habitats in the State of Mexico.

Ornelas-Eusebio E, Obregón-Ascencio A, Chávez-Maya F, García-Espinosa G - J. Vet. Med. Sci. (2014)

Phylogenetic tree of the HA gene of A/environment/Mexico/CPA-04740/2011 (H4N2) [■]Influenza A virus isolated from a wetland. The phylogenetic tree was constructed withthe Neighbor-Joining method. The percentages of replicate trees in which the associatedtaxa clustered together in a bootstrap test (1,000 replicates) are shown next to thebranches. The evolutionary distances were computed using the Kimura 2-parameter method.The analysis involved 45 nucleotide sequences. Evolutionary analyses were conducted inMEGA5.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383787&req=5

fig_001: Phylogenetic tree of the HA gene of A/environment/Mexico/CPA-04740/2011 (H4N2) [■]Influenza A virus isolated from a wetland. The phylogenetic tree was constructed withthe Neighbor-Joining method. The percentages of replicate trees in which the associatedtaxa clustered together in a bootstrap test (1,000 replicates) are shown next to thebranches. The evolutionary distances were computed using the Kimura 2-parameter method.The analysis involved 45 nucleotide sequences. Evolutionary analyses were conducted inMEGA5.05.
Mentions: We found that HA gene of the virus showed 99% similarity to the homologous sequence of thevirus A/blue-winged teal/LA/AI13-1334/2013 (H4N2) [KJ413565.1] and 98% similarity to theviruses A/pekin duck/California/P30/2006 (H4N2) [CY053821.1],A/environment/California/NWRC183970-18/2006 (H4N2) [CY122499.1] and A/environment/NewMexico/NWRC184137-06/2006 (H4N2) [CY122503.1], except for other isolates of the H4 subtypefrom North American wild waterfowl and environmental samples, collected between 1975 and 2009with similarity <97% (Fig. 1Fig. 1.

Bottom Line: This study describes the molecular characterization of an IAV isolated from 240 water samples of a small wetland during non-breeding season of migratory wild ducks in the State of Mexico, Mexico.The results showed that the virus belongs to the H4N2 subtype and each of its eight segments of the viral genome has similarity to IAV isolated from ducks in North America.This study suggests that IAV can be isolated from small wetland during non-breeding season of migrating waterfowl.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Av. Universidad 3000 CP 04510, D.F., México.

ABSTRACT
Wild waterfowl and their habitats are the main reservoirs of influenza A virus (IAV) mainly during the breeding season and prior to migration. This study describes the molecular characterization of an IAV isolated from 240 water samples of a small wetland during non-breeding season of migratory wild ducks in the State of Mexico, Mexico. The results showed that the virus belongs to the H4N2 subtype and each of its eight segments of the viral genome has similarity to IAV isolated from ducks in North America. This study suggests that IAV can be isolated from small wetland during non-breeding season of migrating waterfowl.

Show MeSH
Related in: MedlinePlus