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Isolation and characterization of canine tumor endothelial cells.

Izumi Y, Hoshino Y, Hosoya K, Takagi S, Okumura M - J. Vet. Med. Sci. (2014)

Bottom Line: Further, both TECs and tumor cells derived from a seminoma exhibited increased C-X-C chemokine receptor type 7 (CXCR7) expression.However, CXCR7 expression was not detected in TECs and tumor cells derived from a hepatocellular carcinoma.Understanding TEC specific traits may be important in the development of more efficacious anti-angiogenic therapies that do not induce adverse effects.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.

ABSTRACT
The present study involved the isolation and characterization of canine tumor endothelial cells (TECs) from 2 malignancies. TECs were isolated using magnetic cell sorting following FITC labeling with UEA1 lectin, and they were characterized by measuring genetic and histopathological endothelial markers. Isolated TECs exhibited a cobblestone-like morphology and expressed both vascular endothelial growth factor receptor 2 (VEGFR2) and Von Willebrand factor (vWF). Further, both TECs and tumor cells derived from a seminoma exhibited increased C-X-C chemokine receptor type 7 (CXCR7) expression. However, CXCR7 expression was not detected in TECs and tumor cells derived from a hepatocellular carcinoma. Understanding TEC specific traits may be important in the development of more efficacious anti-angiogenic therapies that do not induce adverse effects.

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Related in: MedlinePlus

Phase contrast microphotographs of the isolated cells obtained from (A) adiposetissues, (B) HCC and (C) Seminoma (Magnification, ×100). All isolated cells showed acobblestone morphology, suggested endothelial cells.
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fig_001: Phase contrast microphotographs of the isolated cells obtained from (A) adiposetissues, (B) HCC and (C) Seminoma (Magnification, ×100). All isolated cells showed acobblestone morphology, suggested endothelial cells.

Mentions: The cells isolated from HCC, seminoma and adipose tissues all exhibited a cobblestonemorphology (Fig. 1Fig. 1.


Isolation and characterization of canine tumor endothelial cells.

Izumi Y, Hoshino Y, Hosoya K, Takagi S, Okumura M - J. Vet. Med. Sci. (2014)

Phase contrast microphotographs of the isolated cells obtained from (A) adiposetissues, (B) HCC and (C) Seminoma (Magnification, ×100). All isolated cells showed acobblestone morphology, suggested endothelial cells.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383786&req=5

fig_001: Phase contrast microphotographs of the isolated cells obtained from (A) adiposetissues, (B) HCC and (C) Seminoma (Magnification, ×100). All isolated cells showed acobblestone morphology, suggested endothelial cells.
Mentions: The cells isolated from HCC, seminoma and adipose tissues all exhibited a cobblestonemorphology (Fig. 1Fig. 1.

Bottom Line: Further, both TECs and tumor cells derived from a seminoma exhibited increased C-X-C chemokine receptor type 7 (CXCR7) expression.However, CXCR7 expression was not detected in TECs and tumor cells derived from a hepatocellular carcinoma.Understanding TEC specific traits may be important in the development of more efficacious anti-angiogenic therapies that do not induce adverse effects.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Veterinary Surgery, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.

ABSTRACT
The present study involved the isolation and characterization of canine tumor endothelial cells (TECs) from 2 malignancies. TECs were isolated using magnetic cell sorting following FITC labeling with UEA1 lectin, and they were characterized by measuring genetic and histopathological endothelial markers. Isolated TECs exhibited a cobblestone-like morphology and expressed both vascular endothelial growth factor receptor 2 (VEGFR2) and Von Willebrand factor (vWF). Further, both TECs and tumor cells derived from a seminoma exhibited increased C-X-C chemokine receptor type 7 (CXCR7) expression. However, CXCR7 expression was not detected in TECs and tumor cells derived from a hepatocellular carcinoma. Understanding TEC specific traits may be important in the development of more efficacious anti-angiogenic therapies that do not induce adverse effects.

Show MeSH
Related in: MedlinePlus