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Change of calbindin D-28k protein expression in the mice hippocampus after lipopolysaccharide treatment.

Chung TH, Choi HS, Lee CH - J. Vet. Med. Sci. (2014)

Bottom Line: CB immunoreactivity was markedly increased in pyramidal cells of the hippocampal CA1/2 regions and in granule cells of the dentate gyrus from 3 hr to 48 hr after LPS treatment.Thereafter, CB protein expression was decreased at 96 hr after LPS treatment.These results indicate that changes of CB protein expression may be associated with no neuronal death in the model of neuroinflammation with systemic administration of 1 mg/kg LPS.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Resources Science, Joongbu University, Chungnam 312-702, South Korea.

ABSTRACT
A previous study showed that 1 mg/kg lipopolysaccharide (LPS) treatment did not lead to the any neuronal death/degeneration in the mouse hippocampus. In the present study, we examined the time-dependent changes of calbindin D-28k (CB) protein expression in the mouse hippocampus after a systemic administration of 1 mg/kg LPS. CB immunoreactivity was markedly increased in pyramidal cells of the hippocampal CA1/2 regions and in granule cells of the dentate gyrus from 3 hr to 48 hr after LPS treatment. At this point in time, CB protein level was also significantly increased in the mouse hippocampus. Thereafter, CB protein expression was decreased at 96 hr after LPS treatment. These results indicate that changes of CB protein expression may be associated with no neuronal death in the model of neuroinflammation with systemic administration of 1 mg/kg LPS.

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Related in: MedlinePlus

Immunohistochemistry for CB in the CA1 region (A–D), the CA3 region (E–H) and thedentate gyrus (I–L) of the control- (A, E and I) and LPS-treated (B–D, F–H andJ–L)-groups. From 3 hr to 48 hr after LPS treatment, CB immunoreactivity is markedlyincreased in the stratum pyramidale (SP, arrows) of the CA1 region, in the stratumlucidum (SL, asterisks) of the CA3 region and in the granule cell layer (GCL,arrowheads) of the dentate gyrus. SO; stratum oriens, SR; stratum radiatum, ML;molecular layer, PL; polymorphic layer. Scale Bar=100 µm.
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fig_001: Immunohistochemistry for CB in the CA1 region (A–D), the CA3 region (E–H) and thedentate gyrus (I–L) of the control- (A, E and I) and LPS-treated (B–D, F–H andJ–L)-groups. From 3 hr to 48 hr after LPS treatment, CB immunoreactivity is markedlyincreased in the stratum pyramidale (SP, arrows) of the CA1 region, in the stratumlucidum (SL, asterisks) of the CA3 region and in the granule cell layer (GCL,arrowheads) of the dentate gyrus. SO; stratum oriens, SR; stratum radiatum, ML;molecular layer, PL; polymorphic layer. Scale Bar=100 µm.

Mentions: Immunoreactivity is scaled as −, ±, + or ++, representing no staining, weakly positive,moderate or strong, respectively.


Change of calbindin D-28k protein expression in the mice hippocampus after lipopolysaccharide treatment.

Chung TH, Choi HS, Lee CH - J. Vet. Med. Sci. (2014)

Immunohistochemistry for CB in the CA1 region (A–D), the CA3 region (E–H) and thedentate gyrus (I–L) of the control- (A, E and I) and LPS-treated (B–D, F–H andJ–L)-groups. From 3 hr to 48 hr after LPS treatment, CB immunoreactivity is markedlyincreased in the stratum pyramidale (SP, arrows) of the CA1 region, in the stratumlucidum (SL, asterisks) of the CA3 region and in the granule cell layer (GCL,arrowheads) of the dentate gyrus. SO; stratum oriens, SR; stratum radiatum, ML;molecular layer, PL; polymorphic layer. Scale Bar=100 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383784&req=5

fig_001: Immunohistochemistry for CB in the CA1 region (A–D), the CA3 region (E–H) and thedentate gyrus (I–L) of the control- (A, E and I) and LPS-treated (B–D, F–H andJ–L)-groups. From 3 hr to 48 hr after LPS treatment, CB immunoreactivity is markedlyincreased in the stratum pyramidale (SP, arrows) of the CA1 region, in the stratumlucidum (SL, asterisks) of the CA3 region and in the granule cell layer (GCL,arrowheads) of the dentate gyrus. SO; stratum oriens, SR; stratum radiatum, ML;molecular layer, PL; polymorphic layer. Scale Bar=100 µm.
Mentions: Immunoreactivity is scaled as −, ±, + or ++, representing no staining, weakly positive,moderate or strong, respectively.

Bottom Line: CB immunoreactivity was markedly increased in pyramidal cells of the hippocampal CA1/2 regions and in granule cells of the dentate gyrus from 3 hr to 48 hr after LPS treatment.Thereafter, CB protein expression was decreased at 96 hr after LPS treatment.These results indicate that changes of CB protein expression may be associated with no neuronal death in the model of neuroinflammation with systemic administration of 1 mg/kg LPS.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Resources Science, Joongbu University, Chungnam 312-702, South Korea.

ABSTRACT
A previous study showed that 1 mg/kg lipopolysaccharide (LPS) treatment did not lead to the any neuronal death/degeneration in the mouse hippocampus. In the present study, we examined the time-dependent changes of calbindin D-28k (CB) protein expression in the mouse hippocampus after a systemic administration of 1 mg/kg LPS. CB immunoreactivity was markedly increased in pyramidal cells of the hippocampal CA1/2 regions and in granule cells of the dentate gyrus from 3 hr to 48 hr after LPS treatment. At this point in time, CB protein level was also significantly increased in the mouse hippocampus. Thereafter, CB protein expression was decreased at 96 hr after LPS treatment. These results indicate that changes of CB protein expression may be associated with no neuronal death in the model of neuroinflammation with systemic administration of 1 mg/kg LPS.

Show MeSH
Related in: MedlinePlus