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Pathogenicity of border disease virus FNK2012-1 strain isolated from a pig in the natural host, sheep.

Tamura T, Mine J, Torii S, Fujimoto Y, Okamatsu M, Sakoda Y - J. Vet. Med. Sci. (2014)

Bottom Line: The inoculated sheep showed clinical symptoms and transient viremia.Seroconversion was observed in the inoculated sheep.Therefore, surveillance of border disease in Japan is necessary among both the swine and ruminant populations.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.

ABSTRACT
A first isolation of border disease virus (BDV) in Japan was from a pig on a farm without keeping any ruminants. Our previous study showed that this BDV, termed the FNK2012-1 strain, replicated inefficiently in swine-derived cells compared with those of ruminant origin. Pigs inoculated with this virus showed neither clinical symptoms nor viremia. In this study, we evaluated the pathogenicity of the FNK2012-1 strain in sheep, its natural host. The inoculated sheep showed clinical symptoms and transient viremia. Seroconversion was observed in the inoculated sheep. These results suggest that the FNK2012-1 strain was introduced from sheep and has not yet adapted to swine. Therefore, surveillance of border disease in Japan is necessary among both the swine and ruminant populations.

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Virus neutralization titers against the FNK2012-1 strain from inoculated lambs. Seracollected on days 0, 1, 5, 7, 10, 15, 22, 25, 28 and 33 pi were used to determine thevirus neutralization titer. “†” indicates the lambs were euthanized at that time.
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fig_003: Virus neutralization titers against the FNK2012-1 strain from inoculated lambs. Seracollected on days 0, 1, 5, 7, 10, 15, 22, 25, 28 and 33 pi were used to determine thevirus neutralization titer. “†” indicates the lambs were euthanized at that time.

Mentions: A neutralization test used to detect anti-BDV, BVDV and CSFV antibodies was performed aspreviously described [7]. Neutralizing antibody titer isexpressed as the reciprocal of the highest serum dilution that showed complete viralneutralization. Seroconversion against the FNK2012-1 strain was detected in the remaining 2lambs (#3 and #4) after day 22 pi (Fig. 3Fig. 3.


Pathogenicity of border disease virus FNK2012-1 strain isolated from a pig in the natural host, sheep.

Tamura T, Mine J, Torii S, Fujimoto Y, Okamatsu M, Sakoda Y - J. Vet. Med. Sci. (2014)

Virus neutralization titers against the FNK2012-1 strain from inoculated lambs. Seracollected on days 0, 1, 5, 7, 10, 15, 22, 25, 28 and 33 pi were used to determine thevirus neutralization titer. “†” indicates the lambs were euthanized at that time.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383782&req=5

fig_003: Virus neutralization titers against the FNK2012-1 strain from inoculated lambs. Seracollected on days 0, 1, 5, 7, 10, 15, 22, 25, 28 and 33 pi were used to determine thevirus neutralization titer. “†” indicates the lambs were euthanized at that time.
Mentions: A neutralization test used to detect anti-BDV, BVDV and CSFV antibodies was performed aspreviously described [7]. Neutralizing antibody titer isexpressed as the reciprocal of the highest serum dilution that showed complete viralneutralization. Seroconversion against the FNK2012-1 strain was detected in the remaining 2lambs (#3 and #4) after day 22 pi (Fig. 3Fig. 3.

Bottom Line: The inoculated sheep showed clinical symptoms and transient viremia.Seroconversion was observed in the inoculated sheep.Therefore, surveillance of border disease in Japan is necessary among both the swine and ruminant populations.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.

ABSTRACT
A first isolation of border disease virus (BDV) in Japan was from a pig on a farm without keeping any ruminants. Our previous study showed that this BDV, termed the FNK2012-1 strain, replicated inefficiently in swine-derived cells compared with those of ruminant origin. Pigs inoculated with this virus showed neither clinical symptoms nor viremia. In this study, we evaluated the pathogenicity of the FNK2012-1 strain in sheep, its natural host. The inoculated sheep showed clinical symptoms and transient viremia. Seroconversion was observed in the inoculated sheep. These results suggest that the FNK2012-1 strain was introduced from sheep and has not yet adapted to swine. Therefore, surveillance of border disease in Japan is necessary among both the swine and ruminant populations.

Show MeSH
Related in: MedlinePlus