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Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens.

Thanasarasakulpong A, Poolperm P, Tankaew P, Sawada T, Sthitmatee N - J. Vet. Med. Sci. (2014)

Bottom Line: Antibody responses in chickens intranasally immunized with rOmpH in combination with 2 different adjuvants were significantly increased (P<0.05) post immunization.Chicken survival rates showed that rOmpH formulated with ODN and LTB elicited 90% and 70% protection, respectively.Our findings indicated that rOmpH formulated with ODN elicited protection better than that formulated with LTB.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.

ABSTRACT
Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine candidate. The present study was aimed at developing rOmpH formulations for intranasal administration. The rOmpH was purified and formulated with either Escherichia coli enterotoxin B (LTB) or CpG oligodeoxynucleotides (ODN) as an adjuvant. Antibody responses in chickens intranasally immunized with rOmpH in combination with 2 different adjuvants were significantly increased (P<0.05) post immunization. Chicken survival rates showed that rOmpH formulated with ODN and LTB elicited 90% and 70% protection, respectively. Our findings indicated that rOmpH formulated with ODN elicited protection better than that formulated with LTB. Therefore, the vaccines formulations in the present study can be considered new intranasal vaccine formulations for fowl cholera in chickens.

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Related in: MedlinePlus

The antibody responses in the sera of chickens immunized with vaccines. (1) rOmpH +ODN via intranasal route, (2) rOmpH + LTB via intranasal route, (3) rOmpH + Freund’sincomplete adjuvant via intramuscular route, (4) DLD bacterin vaccine viaintramuscular route, (5) ODN via intranasal route, (6) LTB via intranasal route and(7) rOmpH without adjuvant via intranasal route. The data were calculated as describedin the test kit’s manual and presented as the log mean end-point titers, and the barsindicate the standard error of the means.
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fig_002: The antibody responses in the sera of chickens immunized with vaccines. (1) rOmpH +ODN via intranasal route, (2) rOmpH + LTB via intranasal route, (3) rOmpH + Freund’sincomplete adjuvant via intramuscular route, (4) DLD bacterin vaccine viaintramuscular route, (5) ODN via intranasal route, (6) LTB via intranasal route and(7) rOmpH without adjuvant via intranasal route. The data were calculated as describedin the test kit’s manual and presented as the log mean end-point titers, and the barsindicate the standard error of the means.

Mentions: Serum IgY profile: Serum IgY was detected in chickens immunized with theintranasal vaccine (Fig. 2Fig. 2.


Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens.

Thanasarasakulpong A, Poolperm P, Tankaew P, Sawada T, Sthitmatee N - J. Vet. Med. Sci. (2014)

The antibody responses in the sera of chickens immunized with vaccines. (1) rOmpH +ODN via intranasal route, (2) rOmpH + LTB via intranasal route, (3) rOmpH + Freund’sincomplete adjuvant via intramuscular route, (4) DLD bacterin vaccine viaintramuscular route, (5) ODN via intranasal route, (6) LTB via intranasal route and(7) rOmpH without adjuvant via intranasal route. The data were calculated as describedin the test kit’s manual and presented as the log mean end-point titers, and the barsindicate the standard error of the means.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383778&req=5

fig_002: The antibody responses in the sera of chickens immunized with vaccines. (1) rOmpH +ODN via intranasal route, (2) rOmpH + LTB via intranasal route, (3) rOmpH + Freund’sincomplete adjuvant via intramuscular route, (4) DLD bacterin vaccine viaintramuscular route, (5) ODN via intranasal route, (6) LTB via intranasal route and(7) rOmpH without adjuvant via intranasal route. The data were calculated as describedin the test kit’s manual and presented as the log mean end-point titers, and the barsindicate the standard error of the means.
Mentions: Serum IgY profile: Serum IgY was detected in chickens immunized with theintranasal vaccine (Fig. 2Fig. 2.

Bottom Line: Antibody responses in chickens intranasally immunized with rOmpH in combination with 2 different adjuvants were significantly increased (P<0.05) post immunization.Chicken survival rates showed that rOmpH formulated with ODN and LTB elicited 90% and 70% protection, respectively.Our findings indicated that rOmpH formulated with ODN elicited protection better than that formulated with LTB.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.

ABSTRACT
Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine candidate. The present study was aimed at developing rOmpH formulations for intranasal administration. The rOmpH was purified and formulated with either Escherichia coli enterotoxin B (LTB) or CpG oligodeoxynucleotides (ODN) as an adjuvant. Antibody responses in chickens intranasally immunized with rOmpH in combination with 2 different adjuvants were significantly increased (P<0.05) post immunization. Chicken survival rates showed that rOmpH formulated with ODN and LTB elicited 90% and 70% protection, respectively. Our findings indicated that rOmpH formulated with ODN elicited protection better than that formulated with LTB. Therefore, the vaccines formulations in the present study can be considered new intranasal vaccine formulations for fowl cholera in chickens.

Show MeSH
Related in: MedlinePlus