Limits...
Nutrient starvation affects expression of LC3 family at the feto-maternal interface during murine placentation.

Hiyama M, Kusakabe KT, Takeshita A, Sugi S, Kuniyoshi N, Imai H, Kano K, Kiso Y - J. Vet. Med. Sci. (2014)

Bottom Line: LC3 - the mammalian homolog of Atg8 - was found as autophagosome membrane binding protein in mammals and widely used as an autophagosomal marker.The results of real time RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by starvation.Taken together, this study suggests that autophagy could play a role in placental invasion system and that nutrient starvation affects LC3B expression.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
LC3 - the mammalian homolog of Atg8 - was found as autophagosome membrane binding protein in mammals and widely used as an autophagosomal marker. LC3A, B and C show different expression patterns in each tissue. The aim of this study was to reveal the differences of expression patterns among LC3 families in mouse placenta under normal condition and nutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A and B were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C was decreased. Starvation induced increase in LC3B expression specifically. Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3A expression in syncytiotrophoblast was vanished by starvation. The results of real time RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by starvation. Taken together, this study suggests that autophagy could play a role in placental invasion system and that nutrient starvation affects LC3B expression.

Show MeSH

Related in: MedlinePlus

Summary of immunohistochemistry of LC3A, B and C. Each positive cell and negativecell is indicated by + and –, respectively. +~– indicates weakly stainabilitycompared with + cells. +/− indicates that both positive and negative cells areobserved.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4383776&req=5

fig_003: Summary of immunohistochemistry of LC3A, B and C. Each positive cell and negativecell is indicated by + and –, respectively. +~– indicates weakly stainabilitycompared with + cells. +/− indicates that both positive and negative cells areobserved.

Mentions: We also observed the expression region of LC3A, B and C by immunohistochemistry. The resultis summarized in Fig. 3Fig. 3.


Nutrient starvation affects expression of LC3 family at the feto-maternal interface during murine placentation.

Hiyama M, Kusakabe KT, Takeshita A, Sugi S, Kuniyoshi N, Imai H, Kano K, Kiso Y - J. Vet. Med. Sci. (2014)

Summary of immunohistochemistry of LC3A, B and C. Each positive cell and negativecell is indicated by + and –, respectively. +~– indicates weakly stainabilitycompared with + cells. +/− indicates that both positive and negative cells areobserved.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383776&req=5

fig_003: Summary of immunohistochemistry of LC3A, B and C. Each positive cell and negativecell is indicated by + and –, respectively. +~– indicates weakly stainabilitycompared with + cells. +/− indicates that both positive and negative cells areobserved.
Mentions: We also observed the expression region of LC3A, B and C by immunohistochemistry. The resultis summarized in Fig. 3Fig. 3.

Bottom Line: LC3 - the mammalian homolog of Atg8 - was found as autophagosome membrane binding protein in mammals and widely used as an autophagosomal marker.The results of real time RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by starvation.Taken together, this study suggests that autophagy could play a role in placental invasion system and that nutrient starvation affects LC3B expression.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Basic Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753-8515, Japan.

ABSTRACT
LC3 - the mammalian homolog of Atg8 - was found as autophagosome membrane binding protein in mammals and widely used as an autophagosomal marker. LC3A, B and C show different expression patterns in each tissue. The aim of this study was to reveal the differences of expression patterns among LC3 families in mouse placenta under normal condition and nutrient starving condition. LC3A and B were highly expressed in decidual cells. LC3A and B were increased in D14 compared with D12 and D16 in mouse placenta, while LC3C was decreased. Starvation induced increase in LC3B expression specifically. Immunohistochemistry showed different expression patterns among LC3A, B and C. LC3A expression in syncytiotrophoblast was vanished by starvation. The results of real time RT-PCR suggested differences between D12 and D16 in autophagic cascade induced by starvation. Taken together, this study suggests that autophagy could play a role in placental invasion system and that nutrient starvation affects LC3B expression.

Show MeSH
Related in: MedlinePlus