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The influences of hyperbaric oxygen therapy with a lower pressure and oxygen concentration than previous methods on physiological mechanisms in dogs.

Ishibashi M, Hayashi A, Akiyoshi H, Ohashi F - J. Vet. Med. Sci. (2014)

Bottom Line: The results showed there were no changes in blood gas parameters, heart rate variability and catecholamine levels after L-HBOT.GPx activity was significantly higher, and the SC and cortisol level were lower in dogs that received L-HBOT than those when they were quiet.These results suggested that L-HBOT may have a small influence on oxygenation dynamics, activate antioxidant enzymes such as GPx, restrain autonomic nervous activity and control the balance between oxidation and antioxidation inside the body.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Clinical Medicine, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-ohrai-kita, Izumisano, Osaka 598-8531, Japan.

ABSTRACT
Recently, hyperbaric oxygen therapy with a lower pressure and oxygen concentration (L-HBOT) than previous methods has been used for dogs in Japan; however, the influences of L-HBOT on dogs have not been clarified. To verify the influences of L-HBOT on physiological mechanism in dogs, we investigated blood gas parameters, glutathione peroxidase (GPx) activity, heart rate variability, stress-related hormones and skin conductance (SC) in 4 clinically normal beagle dogs with catheters in their carotid arteries and jugular veins when they were quiet, after running, after receiving L-HBOT (30% oxygen concentration, 1.3 atmospheres absolute, 30 min) or after not receiving L-HBOT. The results showed there were no changes in blood gas parameters, heart rate variability and catecholamine levels after L-HBOT. GPx activity was significantly higher, and the SC and cortisol level were lower in dogs that received L-HBOT than those when they were quiet. These results suggested that L-HBOT may have a small influence on oxygenation dynamics, activate antioxidant enzymes such as GPx, restrain autonomic nervous activity and control the balance between oxidation and antioxidation inside the body.

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Study protocol. Seven days after catheter placement, we studied the 4 dogs in acrossover trial (1) when the dogs were quiet, (2) after running, (3) after non L-HBOTand (4) after L-HBOT. Briefly, a dog underwent one study protocol and then underwentthe other 7 days later.
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fig_001: Study protocol. Seven days after catheter placement, we studied the 4 dogs in acrossover trial (1) when the dogs were quiet, (2) after running, (3) after non L-HBOTand (4) after L-HBOT. Briefly, a dog underwent one study protocol and then underwentthe other 7 days later.

Mentions: Study protocol: Seven days after catheter placement, we studied the 4 dogsin a crossover trial. We did all studies during the morning to exclude the effects of dailyfluctuation. (1) When the dogs were quiet, we collected blood samples and measuredparameters. (2) Next, the dogs ran on a treadmill (Corpo Motor Walker CP4000, S. N. T. Co.,Ltd., Kanazawa, Japan) at 2.0 m/sec for 10 min to induce physiological stress. Afterrunning, we collected blood samples and measured parameters. Then, the dogs were placedrandomly (3) in a normal cage for 30 min (non L-HBOT) or (4) in a hyperbaric chamber with30% oxygen at 1.3 ATA for 30 min (L-HBOT). After these treatments, wecollected blood samples and measured parameters again. All dogs underwent treatment setsconsisting of (1), (2) and (3) and (1), (2) and (4) randomly. Briefly, a dog underwent onetreatment set and then underwent the other set 7 days later (Fig. 1Fig. 1.


The influences of hyperbaric oxygen therapy with a lower pressure and oxygen concentration than previous methods on physiological mechanisms in dogs.

Ishibashi M, Hayashi A, Akiyoshi H, Ohashi F - J. Vet. Med. Sci. (2014)

Study protocol. Seven days after catheter placement, we studied the 4 dogs in acrossover trial (1) when the dogs were quiet, (2) after running, (3) after non L-HBOTand (4) after L-HBOT. Briefly, a dog underwent one study protocol and then underwentthe other 7 days later.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383775&req=5

fig_001: Study protocol. Seven days after catheter placement, we studied the 4 dogs in acrossover trial (1) when the dogs were quiet, (2) after running, (3) after non L-HBOTand (4) after L-HBOT. Briefly, a dog underwent one study protocol and then underwentthe other 7 days later.
Mentions: Study protocol: Seven days after catheter placement, we studied the 4 dogsin a crossover trial. We did all studies during the morning to exclude the effects of dailyfluctuation. (1) When the dogs were quiet, we collected blood samples and measuredparameters. (2) Next, the dogs ran on a treadmill (Corpo Motor Walker CP4000, S. N. T. Co.,Ltd., Kanazawa, Japan) at 2.0 m/sec for 10 min to induce physiological stress. Afterrunning, we collected blood samples and measured parameters. Then, the dogs were placedrandomly (3) in a normal cage for 30 min (non L-HBOT) or (4) in a hyperbaric chamber with30% oxygen at 1.3 ATA for 30 min (L-HBOT). After these treatments, wecollected blood samples and measured parameters again. All dogs underwent treatment setsconsisting of (1), (2) and (3) and (1), (2) and (4) randomly. Briefly, a dog underwent onetreatment set and then underwent the other set 7 days later (Fig. 1Fig. 1.

Bottom Line: The results showed there were no changes in blood gas parameters, heart rate variability and catecholamine levels after L-HBOT.GPx activity was significantly higher, and the SC and cortisol level were lower in dogs that received L-HBOT than those when they were quiet.These results suggested that L-HBOT may have a small influence on oxygenation dynamics, activate antioxidant enzymes such as GPx, restrain autonomic nervous activity and control the balance between oxidation and antioxidation inside the body.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Clinical Medicine, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-ohrai-kita, Izumisano, Osaka 598-8531, Japan.

ABSTRACT
Recently, hyperbaric oxygen therapy with a lower pressure and oxygen concentration (L-HBOT) than previous methods has been used for dogs in Japan; however, the influences of L-HBOT on dogs have not been clarified. To verify the influences of L-HBOT on physiological mechanism in dogs, we investigated blood gas parameters, glutathione peroxidase (GPx) activity, heart rate variability, stress-related hormones and skin conductance (SC) in 4 clinically normal beagle dogs with catheters in their carotid arteries and jugular veins when they were quiet, after running, after receiving L-HBOT (30% oxygen concentration, 1.3 atmospheres absolute, 30 min) or after not receiving L-HBOT. The results showed there were no changes in blood gas parameters, heart rate variability and catecholamine levels after L-HBOT. GPx activity was significantly higher, and the SC and cortisol level were lower in dogs that received L-HBOT than those when they were quiet. These results suggested that L-HBOT may have a small influence on oxygenation dynamics, activate antioxidant enzymes such as GPx, restrain autonomic nervous activity and control the balance between oxidation and antioxidation inside the body.

Show MeSH
Related in: MedlinePlus