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The canine epiphyseal-derived mesenchymal stem cells are comparable to bone marrow derived-mesenchymal stem cells.

Chang YP, Hong HP, Lee YH, Liu IH - J. Vet. Med. Sci. (2014)

Bottom Line: The successful rate for EMSC isolation is significantly higher compared to BMSC isolation, while the other properties of the isolated MSCs including the clonogenicity, proliferative potentials and molecular phenotypes were not discernibly different between the MSCs established by the two methods.In conclusion, we demonstrated a new procedure to harvest MSCs by bone biopsy at the epiphyseal region.This method is less time consuming and more reliable, and the resulting MSCs are comparable to those harvested by bone marrow aspiration.

View Article: PubMed Central - PubMed

Affiliation: Institute of Veterinary Clinical Science, School of Veterinary Medicine, National Taiwan University, Taipei 106, Taiwan.

ABSTRACT
Mesenchymal stem cells (MSCs) hold great potential in cell therapy and have attracted increasing interests in a wide range of biomedical sciences. However, the scarcity of MSCs and the prolonged isolation procedure limited the clinical application. To address these 2 issues, we developed a method to isolate MSCs from bone biopsy tissues of euthanized canine body donors. Compared to the traditional method to isolate MSCs from aspirated bone marrow (BMSCs), the isolation procedure for MSCs from harvested epiphyseal cancellous bone (EMSCs) was less time-consuming. The isolated EMSCs had similar plastic-adherence, tri-lineage differentiation and consistent surface marker profiles compared to BMSCs. We harvested BMSCs and EMSCs from 24 euthanized cases from clinics and 42 euthanized donors from a local shelter. The successful rate for EMSC isolation is significantly higher compared to BMSC isolation, while the other properties of the isolated MSCs including the clonogenicity, proliferative potentials and molecular phenotypes were not discernibly different between the MSCs established by the two methods. In conclusion, we demonstrated a new procedure to harvest MSCs by bone biopsy at the epiphyseal region. This method is less time consuming and more reliable, and the resulting MSCs are comparable to those harvested by bone marrow aspiration. The combination of the two methods can greatly improve the efficiency to harvest MSCs.

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Successful rates in establishing canine EMSCs and BMSCs. The successful rate wassignificantly lower in the isolation of BMSCs compared to EMSCs at both NTUVH (A) andstray animal shelters (B). * P<0.05
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fig_003: Successful rates in establishing canine EMSCs and BMSCs. The successful rate wassignificantly lower in the isolation of BMSCs compared to EMSCs at both NTUVH (A) andstray animal shelters (B). * P<0.05

Mentions: In this report, the procedure of initial isolation and expansion before any trypsinizationwas defined as passage 0 (P0), which took at most 10 days. A successful establishment ofpassage 1 (P1), i.e., MSCs that showed no sign of contamination, was defined as a successfulisolation. Among 24 patient donors from NTUVH (Fig.3AFig. 3.


The canine epiphyseal-derived mesenchymal stem cells are comparable to bone marrow derived-mesenchymal stem cells.

Chang YP, Hong HP, Lee YH, Liu IH - J. Vet. Med. Sci. (2014)

Successful rates in establishing canine EMSCs and BMSCs. The successful rate wassignificantly lower in the isolation of BMSCs compared to EMSCs at both NTUVH (A) andstray animal shelters (B). * P<0.05
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383772&req=5

fig_003: Successful rates in establishing canine EMSCs and BMSCs. The successful rate wassignificantly lower in the isolation of BMSCs compared to EMSCs at both NTUVH (A) andstray animal shelters (B). * P<0.05
Mentions: In this report, the procedure of initial isolation and expansion before any trypsinizationwas defined as passage 0 (P0), which took at most 10 days. A successful establishment ofpassage 1 (P1), i.e., MSCs that showed no sign of contamination, was defined as a successfulisolation. Among 24 patient donors from NTUVH (Fig.3AFig. 3.

Bottom Line: The successful rate for EMSC isolation is significantly higher compared to BMSC isolation, while the other properties of the isolated MSCs including the clonogenicity, proliferative potentials and molecular phenotypes were not discernibly different between the MSCs established by the two methods.In conclusion, we demonstrated a new procedure to harvest MSCs by bone biopsy at the epiphyseal region.This method is less time consuming and more reliable, and the resulting MSCs are comparable to those harvested by bone marrow aspiration.

View Article: PubMed Central - PubMed

Affiliation: Institute of Veterinary Clinical Science, School of Veterinary Medicine, National Taiwan University, Taipei 106, Taiwan.

ABSTRACT
Mesenchymal stem cells (MSCs) hold great potential in cell therapy and have attracted increasing interests in a wide range of biomedical sciences. However, the scarcity of MSCs and the prolonged isolation procedure limited the clinical application. To address these 2 issues, we developed a method to isolate MSCs from bone biopsy tissues of euthanized canine body donors. Compared to the traditional method to isolate MSCs from aspirated bone marrow (BMSCs), the isolation procedure for MSCs from harvested epiphyseal cancellous bone (EMSCs) was less time-consuming. The isolated EMSCs had similar plastic-adherence, tri-lineage differentiation and consistent surface marker profiles compared to BMSCs. We harvested BMSCs and EMSCs from 24 euthanized cases from clinics and 42 euthanized donors from a local shelter. The successful rate for EMSC isolation is significantly higher compared to BMSC isolation, while the other properties of the isolated MSCs including the clonogenicity, proliferative potentials and molecular phenotypes were not discernibly different between the MSCs established by the two methods. In conclusion, we demonstrated a new procedure to harvest MSCs by bone biopsy at the epiphyseal region. This method is less time consuming and more reliable, and the resulting MSCs are comparable to those harvested by bone marrow aspiration. The combination of the two methods can greatly improve the efficiency to harvest MSCs.

Show MeSH
Related in: MedlinePlus