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Human BDCA2+CD123+CD56+ dendritic cells (DCs) related to blastic plasmacytoid dendritic cell neoplasm represent a unique myeloid DC subset.

Yu H, Zhang P, Yin X, Yin Z, Shi Q, Cui Y, Liu G, Wang S, Piccaluga PP, Jiang T, Zhang L - Protein Cell (2015)

Bottom Line: In addition, CD56(+) DCs cluster together with mDCs but not pDCs by genome-wide transcriptional profiling.These data suggest that the CD56(+) DCs represent a novel mDC subset mixed with some pDC features.However, we demonstrated that BPDCN is closer to CD56(+) DCs than pDCs by global gene-expression profiling.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Immunity and Infection, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.

ABSTRACT
Dendritic cells (DCs) comprise two functionally distinct subsets: plasmacytoid DCs (pDCs) and myeloid DCs (mDCs). pDCs are specialized in rapid and massive secretion of type I interferon (IFN-I) in response to nucleic acids through Toll like receptor (TLR)-7 or TLR-9. In this report, we characterized a CD56(+) DC population that express typical pDC markers including CD123 and BDCA2 but produce much less IFN-I comparing with pDCs. In addition, CD56(+) DCs cluster together with mDCs but not pDCs by genome-wide transcriptional profiling. Accordingly, CD56(+) DCs functionally resemble mDCs by producing IL-12 upon TLR4 stimulation and priming naïve T cells without prior activation. These data suggest that the CD56(+) DCs represent a novel mDC subset mixed with some pDC features. A CD4(+)CD56(+) hematological malignancy was classified as blastic plasmacytoid dendritic cell neoplasm (BPDCN) due to its expression of characteristic molecules of pDCs. However, we demonstrated that BPDCN is closer to CD56(+) DCs than pDCs by global gene-expression profiling. Thus, we propose that the CD4(+)CD56(+) neoplasm may be a tumor counterpart of CD56(+) mDCs but not pDCs.

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Related in: MedlinePlus

Global gene expression of BPDCN, pDCs and CD56+DCs. (A) Heat map of 127 genes highly expressed in BPDCN comparing with pDCs by cDNA array (left panel) and their expressions in CD56+ DCs relative to CD56− pDCs by RNA-Seq (right panel). (B) Heat map of 1143 genes highly expressed in pDCs comparing with BPDCN by cDNA array (left panel) and their expressions in CD56+ DCs relative to CD56− pDCs by RNA-Seq (right panel)
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Fig6: Global gene expression of BPDCN, pDCs and CD56+DCs. (A) Heat map of 127 genes highly expressed in BPDCN comparing with pDCs by cDNA array (left panel) and their expressions in CD56+ DCs relative to CD56− pDCs by RNA-Seq (right panel). (B) Heat map of 1143 genes highly expressed in pDCs comparing with BPDCN by cDNA array (left panel) and their expressions in CD56+ DCs relative to CD56− pDCs by RNA-Seq (right panel)

Mentions: In a previous report, we have found that BPDCN shows both myeloid and pDC characteristics (Cerboni et al., 2013). In order to clarify the relationship among BPDCN, pDC and CD56+ DCs, we analyzed the differentially expressed genes between BPDCN and pDCs. We identified 127 genes express at higher levels in BPDCN than in pDC in cDNA array data (Fig. 6A, left panel; Table S7), but 93 (73%) of them are highly expressed in CD56+ DCs compared to pDCs in our RNA-seq data (Fig. 6A, right panel). Similarly, there are 1143 genes expressed at lower levels in BPDCN cells than in pDCs (Fig. 6B, left panel; Table S8), and 778 (68%) of them are highly expressed in the CD56+ DCs than pDCs (Fig. 6B, right panel). The global gene expression profiling data suggests that BPDCN is more closely related to the CD56+ DCs than pDCs.


Human BDCA2+CD123+CD56+ dendritic cells (DCs) related to blastic plasmacytoid dendritic cell neoplasm represent a unique myeloid DC subset.

Yu H, Zhang P, Yin X, Yin Z, Shi Q, Cui Y, Liu G, Wang S, Piccaluga PP, Jiang T, Zhang L - Protein Cell (2015)

Global gene expression of BPDCN, pDCs and CD56+DCs. (A) Heat map of 127 genes highly expressed in BPDCN comparing with pDCs by cDNA array (left panel) and their expressions in CD56+ DCs relative to CD56− pDCs by RNA-Seq (right panel). (B) Heat map of 1143 genes highly expressed in pDCs comparing with BPDCN by cDNA array (left panel) and their expressions in CD56+ DCs relative to CD56− pDCs by RNA-Seq (right panel)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4383756&req=5

Fig6: Global gene expression of BPDCN, pDCs and CD56+DCs. (A) Heat map of 127 genes highly expressed in BPDCN comparing with pDCs by cDNA array (left panel) and their expressions in CD56+ DCs relative to CD56− pDCs by RNA-Seq (right panel). (B) Heat map of 1143 genes highly expressed in pDCs comparing with BPDCN by cDNA array (left panel) and their expressions in CD56+ DCs relative to CD56− pDCs by RNA-Seq (right panel)
Mentions: In a previous report, we have found that BPDCN shows both myeloid and pDC characteristics (Cerboni et al., 2013). In order to clarify the relationship among BPDCN, pDC and CD56+ DCs, we analyzed the differentially expressed genes between BPDCN and pDCs. We identified 127 genes express at higher levels in BPDCN than in pDC in cDNA array data (Fig. 6A, left panel; Table S7), but 93 (73%) of them are highly expressed in CD56+ DCs compared to pDCs in our RNA-seq data (Fig. 6A, right panel). Similarly, there are 1143 genes expressed at lower levels in BPDCN cells than in pDCs (Fig. 6B, left panel; Table S8), and 778 (68%) of them are highly expressed in the CD56+ DCs than pDCs (Fig. 6B, right panel). The global gene expression profiling data suggests that BPDCN is more closely related to the CD56+ DCs than pDCs.

Bottom Line: In addition, CD56(+) DCs cluster together with mDCs but not pDCs by genome-wide transcriptional profiling.These data suggest that the CD56(+) DCs represent a novel mDC subset mixed with some pDC features.However, we demonstrated that BPDCN is closer to CD56(+) DCs than pDCs by global gene-expression profiling.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Immunity and Infection, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China.

ABSTRACT
Dendritic cells (DCs) comprise two functionally distinct subsets: plasmacytoid DCs (pDCs) and myeloid DCs (mDCs). pDCs are specialized in rapid and massive secretion of type I interferon (IFN-I) in response to nucleic acids through Toll like receptor (TLR)-7 or TLR-9. In this report, we characterized a CD56(+) DC population that express typical pDC markers including CD123 and BDCA2 but produce much less IFN-I comparing with pDCs. In addition, CD56(+) DCs cluster together with mDCs but not pDCs by genome-wide transcriptional profiling. Accordingly, CD56(+) DCs functionally resemble mDCs by producing IL-12 upon TLR4 stimulation and priming naïve T cells without prior activation. These data suggest that the CD56(+) DCs represent a novel mDC subset mixed with some pDC features. A CD4(+)CD56(+) hematological malignancy was classified as blastic plasmacytoid dendritic cell neoplasm (BPDCN) due to its expression of characteristic molecules of pDCs. However, we demonstrated that BPDCN is closer to CD56(+) DCs than pDCs by global gene-expression profiling. Thus, we propose that the CD4(+)CD56(+) neoplasm may be a tumor counterpart of CD56(+) mDCs but not pDCs.

Show MeSH
Related in: MedlinePlus