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Application of GFP imaging in cancer.

Hoffman RM - Lab. Invest. (2015)

Bottom Line: Non-invasive imaging with fluorescent proteins enabled the dynamics of metastatic cancer to be followed in real time in individual animals.Recent applications of the technology described here include linking fluorescent proteins with cell-cycle-specific proteins such that the cells change color from red to green as they transit from G1 to S phases.With the macro- and micro-imaging technologies described here, essentially any in vivo process can be imaged, giving rise to the new field of in vivo cell biology using fluorescent proteins.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer, Inc., Department of Surgery, University of California San Diego, San Diego, CA, USA.

ABSTRACT
Multicolored proteins have allowed the color-coding of cancer cells growing in vivo and enabled the distinction of host from tumor with single-cell resolution. Non-invasive imaging with fluorescent proteins enabled the dynamics of metastatic cancer to be followed in real time in individual animals. Non-invasive imaging of cancer cells expressing fluorescent proteins has allowed the real-time determination of efficacy of candidate antitumor and antimetastatic agents in mouse models. The use of fluorescent proteins to differentially label cancer cells in the nucleus and cytoplasm can visualize the nuclear-cytoplasmic dynamics of cancer cells in vivo including: mitosis, apoptosis, cell-cycle position, and differential behavior of nucleus and cytoplasm that occurs during cancer-cell deformation and extravasation. Recent applications of the technology described here include linking fluorescent proteins with cell-cycle-specific proteins such that the cells change color from red to green as they transit from G1 to S phases. With the macro- and micro-imaging technologies described here, essentially any in vivo process can be imaged, giving rise to the new field of in vivo cell biology using fluorescent proteins.

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Visualization of host macrophage–tumor cell interaction in fresh tumor tissueImages show host macrophages expressing GFP interacting with human PC-3-RFP prostate cancer cells on day 35 after orthotopic implantation of PC-3-RFP cells in the transgenic GFP nude mouse. (A) Host GFP macrophage (arrowhead) contacting RFP cancer cell (arrow). (B) GFP macrophage (arrowhead) engulfing RFP cancer cell (arrow). (C) RFP cancer cell (arrow) engulfed by GFP macrophage (arrowhead). (D) RFP cancer cell (arrows) digested by GFP macrophage (arrowhead). (Scale bars, 20 μm.)81
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Figure 7: Visualization of host macrophage–tumor cell interaction in fresh tumor tissueImages show host macrophages expressing GFP interacting with human PC-3-RFP prostate cancer cells on day 35 after orthotopic implantation of PC-3-RFP cells in the transgenic GFP nude mouse. (A) Host GFP macrophage (arrowhead) contacting RFP cancer cell (arrow). (B) GFP macrophage (arrowhead) engulfing RFP cancer cell (arrow). (C) RFP cancer cell (arrow) engulfed by GFP macrophage (arrowhead). (D) RFP cancer cell (arrows) digested by GFP macrophage (arrowhead). (Scale bars, 20 μm.)81

Mentions: Dual-color fluorescence imaging can be effected by using RFP-expressing tumors growing in GFP-expressing transgenic mice. Tumor–stroma interaction, especially tumor-induced angiogenesis and tumor-infiltrating lymphocytes can be readily imaged in the model. GFP-expressing dendritic cells were observed contacting RFP-expressing tumor cells with their dendrites. GFP-expressing macrophages were observed engulfing RFP-expressing cancer cells. GFP lymphocytes were seen surrounding cells of the RFP tumor, which eventually regressed (Figure 6,7).81


Application of GFP imaging in cancer.

Hoffman RM - Lab. Invest. (2015)

Visualization of host macrophage–tumor cell interaction in fresh tumor tissueImages show host macrophages expressing GFP interacting with human PC-3-RFP prostate cancer cells on day 35 after orthotopic implantation of PC-3-RFP cells in the transgenic GFP nude mouse. (A) Host GFP macrophage (arrowhead) contacting RFP cancer cell (arrow). (B) GFP macrophage (arrowhead) engulfing RFP cancer cell (arrow). (C) RFP cancer cell (arrow) engulfed by GFP macrophage (arrowhead). (D) RFP cancer cell (arrows) digested by GFP macrophage (arrowhead). (Scale bars, 20 μm.)81
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4383682&req=5

Figure 7: Visualization of host macrophage–tumor cell interaction in fresh tumor tissueImages show host macrophages expressing GFP interacting with human PC-3-RFP prostate cancer cells on day 35 after orthotopic implantation of PC-3-RFP cells in the transgenic GFP nude mouse. (A) Host GFP macrophage (arrowhead) contacting RFP cancer cell (arrow). (B) GFP macrophage (arrowhead) engulfing RFP cancer cell (arrow). (C) RFP cancer cell (arrow) engulfed by GFP macrophage (arrowhead). (D) RFP cancer cell (arrows) digested by GFP macrophage (arrowhead). (Scale bars, 20 μm.)81
Mentions: Dual-color fluorescence imaging can be effected by using RFP-expressing tumors growing in GFP-expressing transgenic mice. Tumor–stroma interaction, especially tumor-induced angiogenesis and tumor-infiltrating lymphocytes can be readily imaged in the model. GFP-expressing dendritic cells were observed contacting RFP-expressing tumor cells with their dendrites. GFP-expressing macrophages were observed engulfing RFP-expressing cancer cells. GFP lymphocytes were seen surrounding cells of the RFP tumor, which eventually regressed (Figure 6,7).81

Bottom Line: Non-invasive imaging with fluorescent proteins enabled the dynamics of metastatic cancer to be followed in real time in individual animals.Recent applications of the technology described here include linking fluorescent proteins with cell-cycle-specific proteins such that the cells change color from red to green as they transit from G1 to S phases.With the macro- and micro-imaging technologies described here, essentially any in vivo process can be imaged, giving rise to the new field of in vivo cell biology using fluorescent proteins.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer, Inc., Department of Surgery, University of California San Diego, San Diego, CA, USA.

ABSTRACT
Multicolored proteins have allowed the color-coding of cancer cells growing in vivo and enabled the distinction of host from tumor with single-cell resolution. Non-invasive imaging with fluorescent proteins enabled the dynamics of metastatic cancer to be followed in real time in individual animals. Non-invasive imaging of cancer cells expressing fluorescent proteins has allowed the real-time determination of efficacy of candidate antitumor and antimetastatic agents in mouse models. The use of fluorescent proteins to differentially label cancer cells in the nucleus and cytoplasm can visualize the nuclear-cytoplasmic dynamics of cancer cells in vivo including: mitosis, apoptosis, cell-cycle position, and differential behavior of nucleus and cytoplasm that occurs during cancer-cell deformation and extravasation. Recent applications of the technology described here include linking fluorescent proteins with cell-cycle-specific proteins such that the cells change color from red to green as they transit from G1 to S phases. With the macro- and micro-imaging technologies described here, essentially any in vivo process can be imaged, giving rise to the new field of in vivo cell biology using fluorescent proteins.

Show MeSH
Related in: MedlinePlus