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Application of GFP imaging in cancer.

Hoffman RM - Lab. Invest. (2015)

Bottom Line: Non-invasive imaging with fluorescent proteins enabled the dynamics of metastatic cancer to be followed in real time in individual animals.Recent applications of the technology described here include linking fluorescent proteins with cell-cycle-specific proteins such that the cells change color from red to green as they transit from G1 to S phases.With the macro- and micro-imaging technologies described here, essentially any in vivo process can be imaged, giving rise to the new field of in vivo cell biology using fluorescent proteins.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer, Inc., Department of Surgery, University of California San Diego, San Diego, CA, USA.

ABSTRACT
Multicolored proteins have allowed the color-coding of cancer cells growing in vivo and enabled the distinction of host from tumor with single-cell resolution. Non-invasive imaging with fluorescent proteins enabled the dynamics of metastatic cancer to be followed in real time in individual animals. Non-invasive imaging of cancer cells expressing fluorescent proteins has allowed the real-time determination of efficacy of candidate antitumor and antimetastatic agents in mouse models. The use of fluorescent proteins to differentially label cancer cells in the nucleus and cytoplasm can visualize the nuclear-cytoplasmic dynamics of cancer cells in vivo including: mitosis, apoptosis, cell-cycle position, and differential behavior of nucleus and cytoplasm that occurs during cancer-cell deformation and extravasation. Recent applications of the technology described here include linking fluorescent proteins with cell-cycle-specific proteins such that the cells change color from red to green as they transit from G1 to S phases. With the macro- and micro-imaging technologies described here, essentially any in vivo process can be imaged, giving rise to the new field of in vivo cell biology using fluorescent proteins.

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Invasive cancer cells are predominantly in G0/G1FUCCI-expressing cancer cells (5 × 106) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. (A) High-magnification real-time images of invading cancer cells cultured on Gelfoam® for 48 h. Arrows show the direction of invading cancer cells. (B) Histogram shows cell cycle phase of invading cancer cells.78
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Figure 4: Invasive cancer cells are predominantly in G0/G1FUCCI-expressing cancer cells (5 × 106) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. (A) High-magnification real-time images of invading cancer cells cultured on Gelfoam® for 48 h. Arrows show the direction of invading cancer cells. (B) Histogram shows cell cycle phase of invading cancer cells.78

Mentions: FUCCI 3D confocal imaging visualized that cancer cells in G0/G1 phase in Gelfoam® histoculture migrated more rapidly and further than cancer cells in S/G2/M phases. Cancer cells ceased migrating when they entered S/G2/M phases and restarted migrating after cell division when the cells re-entered G0/G1. Migrating cancer cells also were resistant to cytotoxic chemotherapy, since they were preponderantly in G0/G1, where cytotoxic chemotherapy is not effective (Figure 4).78


Application of GFP imaging in cancer.

Hoffman RM - Lab. Invest. (2015)

Invasive cancer cells are predominantly in G0/G1FUCCI-expressing cancer cells (5 × 106) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. (A) High-magnification real-time images of invading cancer cells cultured on Gelfoam® for 48 h. Arrows show the direction of invading cancer cells. (B) Histogram shows cell cycle phase of invading cancer cells.78
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4383682&req=5

Figure 4: Invasive cancer cells are predominantly in G0/G1FUCCI-expressing cancer cells (5 × 106) were placed on Gelfoam® (1 × 1 cm) in RPMI 1640 medium. (A) High-magnification real-time images of invading cancer cells cultured on Gelfoam® for 48 h. Arrows show the direction of invading cancer cells. (B) Histogram shows cell cycle phase of invading cancer cells.78
Mentions: FUCCI 3D confocal imaging visualized that cancer cells in G0/G1 phase in Gelfoam® histoculture migrated more rapidly and further than cancer cells in S/G2/M phases. Cancer cells ceased migrating when they entered S/G2/M phases and restarted migrating after cell division when the cells re-entered G0/G1. Migrating cancer cells also were resistant to cytotoxic chemotherapy, since they were preponderantly in G0/G1, where cytotoxic chemotherapy is not effective (Figure 4).78

Bottom Line: Non-invasive imaging with fluorescent proteins enabled the dynamics of metastatic cancer to be followed in real time in individual animals.Recent applications of the technology described here include linking fluorescent proteins with cell-cycle-specific proteins such that the cells change color from red to green as they transit from G1 to S phases.With the macro- and micro-imaging technologies described here, essentially any in vivo process can be imaged, giving rise to the new field of in vivo cell biology using fluorescent proteins.

View Article: PubMed Central - PubMed

Affiliation: AntiCancer, Inc., Department of Surgery, University of California San Diego, San Diego, CA, USA.

ABSTRACT
Multicolored proteins have allowed the color-coding of cancer cells growing in vivo and enabled the distinction of host from tumor with single-cell resolution. Non-invasive imaging with fluorescent proteins enabled the dynamics of metastatic cancer to be followed in real time in individual animals. Non-invasive imaging of cancer cells expressing fluorescent proteins has allowed the real-time determination of efficacy of candidate antitumor and antimetastatic agents in mouse models. The use of fluorescent proteins to differentially label cancer cells in the nucleus and cytoplasm can visualize the nuclear-cytoplasmic dynamics of cancer cells in vivo including: mitosis, apoptosis, cell-cycle position, and differential behavior of nucleus and cytoplasm that occurs during cancer-cell deformation and extravasation. Recent applications of the technology described here include linking fluorescent proteins with cell-cycle-specific proteins such that the cells change color from red to green as they transit from G1 to S phases. With the macro- and micro-imaging technologies described here, essentially any in vivo process can be imaged, giving rise to the new field of in vivo cell biology using fluorescent proteins.

Show MeSH
Related in: MedlinePlus