Treadmill exercise induces murine cardiac allograft survival and generates regulatory T cell.
Bottom Line: Adoptive transfer of whole splenocytes and CD4(+) cells from treadmill exercise recipients significantly prolonged allograft survival in naive secondary recipients (MSTs, 30 and 52 days, respectively), suggesting that regulatory cells was generated after treadmill exercise.Moreover, flow cytometry studies showed that CD4(+) CD25(+) Foxp3(+) cell population increased in treadmill exercise recipients.Therefore, postoperative but not pre-operative exercise could induce prolongation of survival of fully allogeneic cardiac allografts and generate CD4(+) CD25(+) Foxp3(+) regulatory T cells.
Affiliation: Department of Cardiovascular Surgery, Teikyo University, Tokyo, Japan; Department of Surgery, Teikyo University, Tokyo, Japan.Show MeSH
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Mentions: Adoptive transfer studies were conducted to determine whether regulatory cells were generated in mice exercised on a treadmill. Thus, 30 days after transplantation of B6 hearts into primary CBA recipients exercised on a treadmill for 1 week after grafting, splenocytes (5.0 × 107) from primary recipients with functioning allografts were adoptively transferred into naïve secondary CBA recipients by means of intravenous injection into the penile vein. Immediately afterward, the secondary recipients underwent transplantation of a B6 heart. In some experiments, CD4+ cells were purified from the spleens of primary transplant recipients by positive selection using a magnetically activated cell sorter (MACS) and CD4 microbeads (Miltenyi Biotec, Auburn, CA; purity >98%), and CD4+ cells (2.0 × 107) were adoptively transferred into naïve secondary recipients, which then immediately underwent transplantation of a B6 heart (Fig.2a).
Affiliation: Department of Cardiovascular Surgery, Teikyo University, Tokyo, Japan; Department of Surgery, Teikyo University, Tokyo, Japan.