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The extent of irradiation-induced long-term visceral organ damage depends on cranial/brain exposure.

Boittin FX, Denis J, Mayol JF, Martigne P, Raffin F, Coulon D, Grenier N, Drouet M, Hérodin F - PLoS ONE (2015)

Bottom Line: To investigate the influence of cranial/brain irradiation on late visceral organ damage in case of high-dose exposure, Wistar rats were irradiated at 12 Gy, with either the head and fore limbs or the two hind limbs protected behind a lead wall (head- and hind limbs-protected respectively), which allows long-term survival thanks to bone marrow protection.Histological analysis performed at this time revealed that late damages to liver, kidney and ileum were attenuated in rats with head exposed when compared to animals whose head was protected.Altogether our results demonstrate the influence of cranial/brain exposure in the onset of organ damage.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiobiology, IRBA (Institut de Recherche Biomédicale des Armées), Brétigny-sur-Orge, France.

ABSTRACT
In case of high-dose radiation exposure, mechanisms controlling late visceral organ damage are still not completely understood and may involve the central nervous system. To investigate the influence of cranial/brain irradiation on late visceral organ damage in case of high-dose exposure, Wistar rats were irradiated at 12 Gy, with either the head and fore limbs or the two hind limbs protected behind a lead wall (head- and hind limbs-protected respectively), which allows long-term survival thanks to bone marrow protection. Although hind limbs- and head-protected irradiated rats exhibited similar hematopoietic and spleen reconstitution, a late body weight loss was observed in hind limbs-protected rats only. Histological analysis performed at this time revealed that late damages to liver, kidney and ileum were attenuated in rats with head exposed when compared to animals whose head was protected. Plasma measurements of inflammation biomarkers (haptoglobin and the chemokine CXCL1) suggest that the attenuated organ damage in hind limbs-protected rats may be in part related to reduced acute and chronic inflammation. Altogether our results demonstrate the influence of cranial/brain exposure in the onset of organ damage.

No MeSH data available.


Related in: MedlinePlus

Kinetic analysis of plasma haptoglobin and corticosterone levels in 12 Gy-irradiated rats with hind limbs or head protection.A: Plasma haptoglobin level was measured using a Hitachi 912 automatic analyser. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of 4–5 hind limbs-protected rats, 4–5 head-protected rats and 5 age-matched control rats from the same batch. All animals were treated with Enrofloxacin. Insert shows average values of haptoglobin plasma levels obtained by pooling data from 10 to 101 days post-irradiation. The number of rats used is indicated on the bar chart. B: Plasma corticosterone levels were measured using enzyme immunoassays (EIA) kits. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of hind limbs-protected rats, head-protected rats and age-matched control rats from the same batch. All animals were treated with Enrofloxacin. (*** (p<0.001); ** (p<0.01); * (p<0.05); n.s.: not significant).
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pone.0122900.g005: Kinetic analysis of plasma haptoglobin and corticosterone levels in 12 Gy-irradiated rats with hind limbs or head protection.A: Plasma haptoglobin level was measured using a Hitachi 912 automatic analyser. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of 4–5 hind limbs-protected rats, 4–5 head-protected rats and 5 age-matched control rats from the same batch. All animals were treated with Enrofloxacin. Insert shows average values of haptoglobin plasma levels obtained by pooling data from 10 to 101 days post-irradiation. The number of rats used is indicated on the bar chart. B: Plasma corticosterone levels were measured using enzyme immunoassays (EIA) kits. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of hind limbs-protected rats, head-protected rats and age-matched control rats from the same batch. All animals were treated with Enrofloxacin. (*** (p<0.001); ** (p<0.01); * (p<0.05); n.s.: not significant).

Mentions: Kinetic analysis of plasma haptoglobin levels was performed in control and 12 Gy-irradiated rats with hind limbs or head protection. Haptoglobin is an acute phase protein of inflammatory reaction and a reliable marker of inflammation [26,27]. Plasma haptoglobin level was found to be greatly and significantly increased in both hind limbs- and head-protected rats 1 day post-irradiation, when compared to controls (Fig. 5A). However, while haptoglobin level returned to a value close to control in the case of hind limbs-protected rats 4 days post-irradiation, it remained significantly increased in head-protected rats, suggesting prolonged acute inflammatory reaction in head-protected rats (Fig. 5A). In both hind limbs- and head-protected rats, plasma haptoglobin levels returned to control levels 10 days post-irradiation. Insert in Fig. 5A shows average plasma haptoglobin values for control and irradiated rats during the late phase of irradiation. These results indicate that between 10 and 101 days post-irradiation, average plasma haptoglobin levels were significantly increased in both groups of irradiated rats when compared to controls. Therefore this indicates that late chronic inflammation developed in both groups of irradiated animals.


The extent of irradiation-induced long-term visceral organ damage depends on cranial/brain exposure.

Boittin FX, Denis J, Mayol JF, Martigne P, Raffin F, Coulon D, Grenier N, Drouet M, Hérodin F - PLoS ONE (2015)

Kinetic analysis of plasma haptoglobin and corticosterone levels in 12 Gy-irradiated rats with hind limbs or head protection.A: Plasma haptoglobin level was measured using a Hitachi 912 automatic analyser. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of 4–5 hind limbs-protected rats, 4–5 head-protected rats and 5 age-matched control rats from the same batch. All animals were treated with Enrofloxacin. Insert shows average values of haptoglobin plasma levels obtained by pooling data from 10 to 101 days post-irradiation. The number of rats used is indicated on the bar chart. B: Plasma corticosterone levels were measured using enzyme immunoassays (EIA) kits. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of hind limbs-protected rats, head-protected rats and age-matched control rats from the same batch. All animals were treated with Enrofloxacin. (*** (p<0.001); ** (p<0.01); * (p<0.05); n.s.: not significant).
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getmorefigures.php?uid=PMC4383625&req=5

pone.0122900.g005: Kinetic analysis of plasma haptoglobin and corticosterone levels in 12 Gy-irradiated rats with hind limbs or head protection.A: Plasma haptoglobin level was measured using a Hitachi 912 automatic analyser. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of 4–5 hind limbs-protected rats, 4–5 head-protected rats and 5 age-matched control rats from the same batch. All animals were treated with Enrofloxacin. Insert shows average values of haptoglobin plasma levels obtained by pooling data from 10 to 101 days post-irradiation. The number of rats used is indicated on the bar chart. B: Plasma corticosterone levels were measured using enzyme immunoassays (EIA) kits. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of hind limbs-protected rats, head-protected rats and age-matched control rats from the same batch. All animals were treated with Enrofloxacin. (*** (p<0.001); ** (p<0.01); * (p<0.05); n.s.: not significant).
Mentions: Kinetic analysis of plasma haptoglobin levels was performed in control and 12 Gy-irradiated rats with hind limbs or head protection. Haptoglobin is an acute phase protein of inflammatory reaction and a reliable marker of inflammation [26,27]. Plasma haptoglobin level was found to be greatly and significantly increased in both hind limbs- and head-protected rats 1 day post-irradiation, when compared to controls (Fig. 5A). However, while haptoglobin level returned to a value close to control in the case of hind limbs-protected rats 4 days post-irradiation, it remained significantly increased in head-protected rats, suggesting prolonged acute inflammatory reaction in head-protected rats (Fig. 5A). In both hind limbs- and head-protected rats, plasma haptoglobin levels returned to control levels 10 days post-irradiation. Insert in Fig. 5A shows average plasma haptoglobin values for control and irradiated rats during the late phase of irradiation. These results indicate that between 10 and 101 days post-irradiation, average plasma haptoglobin levels were significantly increased in both groups of irradiated rats when compared to controls. Therefore this indicates that late chronic inflammation developed in both groups of irradiated animals.

Bottom Line: To investigate the influence of cranial/brain irradiation on late visceral organ damage in case of high-dose exposure, Wistar rats were irradiated at 12 Gy, with either the head and fore limbs or the two hind limbs protected behind a lead wall (head- and hind limbs-protected respectively), which allows long-term survival thanks to bone marrow protection.Histological analysis performed at this time revealed that late damages to liver, kidney and ileum were attenuated in rats with head exposed when compared to animals whose head was protected.Altogether our results demonstrate the influence of cranial/brain exposure in the onset of organ damage.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiobiology, IRBA (Institut de Recherche Biomédicale des Armées), Brétigny-sur-Orge, France.

ABSTRACT
In case of high-dose radiation exposure, mechanisms controlling late visceral organ damage are still not completely understood and may involve the central nervous system. To investigate the influence of cranial/brain irradiation on late visceral organ damage in case of high-dose exposure, Wistar rats were irradiated at 12 Gy, with either the head and fore limbs or the two hind limbs protected behind a lead wall (head- and hind limbs-protected respectively), which allows long-term survival thanks to bone marrow protection. Although hind limbs- and head-protected irradiated rats exhibited similar hematopoietic and spleen reconstitution, a late body weight loss was observed in hind limbs-protected rats only. Histological analysis performed at this time revealed that late damages to liver, kidney and ileum were attenuated in rats with head exposed when compared to animals whose head was protected. Plasma measurements of inflammation biomarkers (haptoglobin and the chemokine CXCL1) suggest that the attenuated organ damage in hind limbs-protected rats may be in part related to reduced acute and chronic inflammation. Altogether our results demonstrate the influence of cranial/brain exposure in the onset of organ damage.

No MeSH data available.


Related in: MedlinePlus