Limits...
The extent of irradiation-induced long-term visceral organ damage depends on cranial/brain exposure.

Boittin FX, Denis J, Mayol JF, Martigne P, Raffin F, Coulon D, Grenier N, Drouet M, Hérodin F - PLoS ONE (2015)

Bottom Line: To investigate the influence of cranial/brain irradiation on late visceral organ damage in case of high-dose exposure, Wistar rats were irradiated at 12 Gy, with either the head and fore limbs or the two hind limbs protected behind a lead wall (head- and hind limbs-protected respectively), which allows long-term survival thanks to bone marrow protection.Histological analysis performed at this time revealed that late damages to liver, kidney and ileum were attenuated in rats with head exposed when compared to animals whose head was protected.Altogether our results demonstrate the influence of cranial/brain exposure in the onset of organ damage.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiobiology, IRBA (Institut de Recherche Biomédicale des Armées), Brétigny-sur-Orge, France.

ABSTRACT
In case of high-dose radiation exposure, mechanisms controlling late visceral organ damage are still not completely understood and may involve the central nervous system. To investigate the influence of cranial/brain irradiation on late visceral organ damage in case of high-dose exposure, Wistar rats were irradiated at 12 Gy, with either the head and fore limbs or the two hind limbs protected behind a lead wall (head- and hind limbs-protected respectively), which allows long-term survival thanks to bone marrow protection. Although hind limbs- and head-protected irradiated rats exhibited similar hematopoietic and spleen reconstitution, a late body weight loss was observed in hind limbs-protected rats only. Histological analysis performed at this time revealed that late damages to liver, kidney and ileum were attenuated in rats with head exposed when compared to animals whose head was protected. Plasma measurements of inflammation biomarkers (haptoglobin and the chemokine CXCL1) suggest that the attenuated organ damage in hind limbs-protected rats may be in part related to reduced acute and chronic inflammation. Altogether our results demonstrate the influence of cranial/brain exposure in the onset of organ damage.

No MeSH data available.


Related in: MedlinePlus

Evolution of body weight and plasma albumin levels in 12 Gy-irradiated rats with hind limbs or head protection.A: Body weight evolution for controls (5 rats) and 12 Gy-irradiated rats with either hind limbs (5 rats) or head protected (4 rats). All rats received antibiotic support (Enrofloxacin). Measurements of body weight were performed every 2–3 days. The arrow on the graph indicates late average body weight loss for hind limbs-protected rats. The evolution of body weight is represented for rat groups euthanized 101 days after irradiation. The same body weight evolution was observed for all groups of hind limbs-, head-protected and control rats euthanized earlier after irradiation. (*** (p0.001) significant versus control + enrofloxacin; § (p<0.05): significant versus head-protected). B: Kinetic analysis of plasma albumin levels in 12 Gy-irradiated rats with hind limbs or head protection. Plasma albumin levels were measured using a Hitachi 912 automatic analyser. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of 4–5 hind limbs-protected rats, 4–5 head-protected rats and 5 age-matched control rats from the same batch. All animals were treated with Enrofloxacin. (** (p<0.01); * (p<0.05); n.s.: not significant).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4383625&req=5

pone.0122900.g002: Evolution of body weight and plasma albumin levels in 12 Gy-irradiated rats with hind limbs or head protection.A: Body weight evolution for controls (5 rats) and 12 Gy-irradiated rats with either hind limbs (5 rats) or head protected (4 rats). All rats received antibiotic support (Enrofloxacin). Measurements of body weight were performed every 2–3 days. The arrow on the graph indicates late average body weight loss for hind limbs-protected rats. The evolution of body weight is represented for rat groups euthanized 101 days after irradiation. The same body weight evolution was observed for all groups of hind limbs-, head-protected and control rats euthanized earlier after irradiation. (*** (p0.001) significant versus control + enrofloxacin; § (p<0.05): significant versus head-protected). B: Kinetic analysis of plasma albumin levels in 12 Gy-irradiated rats with hind limbs or head protection. Plasma albumin levels were measured using a Hitachi 912 automatic analyser. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of 4–5 hind limbs-protected rats, 4–5 head-protected rats and 5 age-matched control rats from the same batch. All animals were treated with Enrofloxacin. (** (p<0.01); * (p<0.05); n.s.: not significant).

Mentions: Control and irradiated rats were weighed every 2–3 days. As shown in Fig. 2A, both hind limbs- and head-protected rats exhibited severe and rapid body weight loss until 8 days post-irradiation, due to acute radiation syndrome. This was followed by a recovery phase for both groups of irradiated rats. However, a second late phase of body weight loss, starting about 45 days post-irradiation, was observed in hind limbs-protected rats only (arrow in Fig. 2A). At 59 days post-irradiation, average body weight of hind limbs-protected rats was significantly reduced when compared to head-protected animals, while average body weight of both hind limbs- and head-protected irradiated rats was significantly reduced when compared to age-matched controls. Age-matched control rats receiving antibiotic support exhibited continuous body weight increase throughout the time course of experiments, as can be expected from rats fed ad libitum (Fig. 2A).


The extent of irradiation-induced long-term visceral organ damage depends on cranial/brain exposure.

Boittin FX, Denis J, Mayol JF, Martigne P, Raffin F, Coulon D, Grenier N, Drouet M, Hérodin F - PLoS ONE (2015)

Evolution of body weight and plasma albumin levels in 12 Gy-irradiated rats with hind limbs or head protection.A: Body weight evolution for controls (5 rats) and 12 Gy-irradiated rats with either hind limbs (5 rats) or head protected (4 rats). All rats received antibiotic support (Enrofloxacin). Measurements of body weight were performed every 2–3 days. The arrow on the graph indicates late average body weight loss for hind limbs-protected rats. The evolution of body weight is represented for rat groups euthanized 101 days after irradiation. The same body weight evolution was observed for all groups of hind limbs-, head-protected and control rats euthanized earlier after irradiation. (*** (p0.001) significant versus control + enrofloxacin; § (p<0.05): significant versus head-protected). B: Kinetic analysis of plasma albumin levels in 12 Gy-irradiated rats with hind limbs or head protection. Plasma albumin levels were measured using a Hitachi 912 automatic analyser. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of 4–5 hind limbs-protected rats, 4–5 head-protected rats and 5 age-matched control rats from the same batch. All animals were treated with Enrofloxacin. (** (p<0.01); * (p<0.05); n.s.: not significant).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383625&req=5

pone.0122900.g002: Evolution of body weight and plasma albumin levels in 12 Gy-irradiated rats with hind limbs or head protection.A: Body weight evolution for controls (5 rats) and 12 Gy-irradiated rats with either hind limbs (5 rats) or head protected (4 rats). All rats received antibiotic support (Enrofloxacin). Measurements of body weight were performed every 2–3 days. The arrow on the graph indicates late average body weight loss for hind limbs-protected rats. The evolution of body weight is represented for rat groups euthanized 101 days after irradiation. The same body weight evolution was observed for all groups of hind limbs-, head-protected and control rats euthanized earlier after irradiation. (*** (p0.001) significant versus control + enrofloxacin; § (p<0.05): significant versus head-protected). B: Kinetic analysis of plasma albumin levels in 12 Gy-irradiated rats with hind limbs or head protection. Plasma albumin levels were measured using a Hitachi 912 automatic analyser. For each day post-irradiation (1, 4, 10, 21, 31, 37, 50, 59, 71, 101), data represent average values obtained from the plasma of 4–5 hind limbs-protected rats, 4–5 head-protected rats and 5 age-matched control rats from the same batch. All animals were treated with Enrofloxacin. (** (p<0.01); * (p<0.05); n.s.: not significant).
Mentions: Control and irradiated rats were weighed every 2–3 days. As shown in Fig. 2A, both hind limbs- and head-protected rats exhibited severe and rapid body weight loss until 8 days post-irradiation, due to acute radiation syndrome. This was followed by a recovery phase for both groups of irradiated rats. However, a second late phase of body weight loss, starting about 45 days post-irradiation, was observed in hind limbs-protected rats only (arrow in Fig. 2A). At 59 days post-irradiation, average body weight of hind limbs-protected rats was significantly reduced when compared to head-protected animals, while average body weight of both hind limbs- and head-protected irradiated rats was significantly reduced when compared to age-matched controls. Age-matched control rats receiving antibiotic support exhibited continuous body weight increase throughout the time course of experiments, as can be expected from rats fed ad libitum (Fig. 2A).

Bottom Line: To investigate the influence of cranial/brain irradiation on late visceral organ damage in case of high-dose exposure, Wistar rats were irradiated at 12 Gy, with either the head and fore limbs or the two hind limbs protected behind a lead wall (head- and hind limbs-protected respectively), which allows long-term survival thanks to bone marrow protection.Histological analysis performed at this time revealed that late damages to liver, kidney and ileum were attenuated in rats with head exposed when compared to animals whose head was protected.Altogether our results demonstrate the influence of cranial/brain exposure in the onset of organ damage.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiobiology, IRBA (Institut de Recherche Biomédicale des Armées), Brétigny-sur-Orge, France.

ABSTRACT
In case of high-dose radiation exposure, mechanisms controlling late visceral organ damage are still not completely understood and may involve the central nervous system. To investigate the influence of cranial/brain irradiation on late visceral organ damage in case of high-dose exposure, Wistar rats were irradiated at 12 Gy, with either the head and fore limbs or the two hind limbs protected behind a lead wall (head- and hind limbs-protected respectively), which allows long-term survival thanks to bone marrow protection. Although hind limbs- and head-protected irradiated rats exhibited similar hematopoietic and spleen reconstitution, a late body weight loss was observed in hind limbs-protected rats only. Histological analysis performed at this time revealed that late damages to liver, kidney and ileum were attenuated in rats with head exposed when compared to animals whose head was protected. Plasma measurements of inflammation biomarkers (haptoglobin and the chemokine CXCL1) suggest that the attenuated organ damage in hind limbs-protected rats may be in part related to reduced acute and chronic inflammation. Altogether our results demonstrate the influence of cranial/brain exposure in the onset of organ damage.

No MeSH data available.


Related in: MedlinePlus