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Association between ankylosing spondylitis and the miR-146a and miR-499 polymorphisms.

Xu HY, Wang ZY, Chen JF, Wang TY, Wang LL, Tang LL, Lin XY, Zhang CW, Chen BC - PLoS ONE (2015)

Bottom Line: A significant difference was observed in the miR-146a rs2910164G>C SNP.However, no significant association was found between the miR-499 rs3746444T>C variant and susceptibility to AS.Further studies are needed to validate our findings in a larger series, as well as in other ethnic backgrounds.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.

ABSTRACT
miRNAs are small, non-coding RNAs that regulate the expression of multiple target genes at the post-transcriptional level. Single-nucleotide polymorphisms (SNPs) in miRNA sequences may alter miRNA expression and have been implicated in the pathogenesis of multiple forms of arthritis, including rheumatoid arthritis (RA) and osteoarthritis. The present study explored the association between ankylosing spondylitis (AS) and two single nucleotide polymorphisms (SNPs), miR-146a rs2910164G>C and miR-499 rs3746444T>C, in a Han Chinese population. A case-control study consisting of 102 subjects with AS and 105 healthy controls was designed. The two miRNA SNPs were identified by direct sequencing. Subsequently, their gene and genotype frequencies were compared with healthy controls. A significant difference was observed in the miR-146a rs2910164G>C SNP. The frequency of the G allele was markedly higher in the AS patients than in the healthy controls (P = 0.005, Pc = 0.01, OR = 1.787), and the frequency of the GG genotype was higher in AS patients than in controls (P = 0.014, Pc = 0.042, OR = 2.516). However, no significant association was found between the miR-499 rs3746444T>C variant and susceptibility to AS. This is the first study to address the association between the miR-146a rs2910164G>C and miR-499 rs3746444T>C polymorphisms and AS, and it suggests a potential pathogenic factor for AS. Further studies are needed to validate our findings in a larger series, as well as in other ethnic backgrounds.

No MeSH data available.


Related in: MedlinePlus

Association between variants of miR-146a (rs2910164) and miR-499 (rs3746444) and activity parameters.A. The levels of CRP among different miR-146a genotypes. No significant differences were found (CC vs. GG, P = 0.43; CC vs. GC, P = 0.79; GG vs. GC, P = 0.515). B. The levels of ESR among miR-146a genotypes. There were no significant differences among the three genotypes (CC vs. GG, P = 0.222; CC vs. GC, P = 0.249; GG vs. GC, P = 0.782). C. The levels of CRP among different miR-499 genotypes. No significant differences were found (TT vs. TC, P = 0.607). D. The levels of ESR among miR-499 genotypes. There were no significant differences (TT vs. TC, P = 0.759).
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pone.0122055.g001: Association between variants of miR-146a (rs2910164) and miR-499 (rs3746444) and activity parameters.A. The levels of CRP among different miR-146a genotypes. No significant differences were found (CC vs. GG, P = 0.43; CC vs. GC, P = 0.79; GG vs. GC, P = 0.515). B. The levels of ESR among miR-146a genotypes. There were no significant differences among the three genotypes (CC vs. GG, P = 0.222; CC vs. GC, P = 0.249; GG vs. GC, P = 0.782). C. The levels of CRP among different miR-499 genotypes. No significant differences were found (TT vs. TC, P = 0.607). D. The levels of ESR among miR-499 genotypes. There were no significant differences (TT vs. TC, P = 0.759).

Mentions: As shown in Fig. 1, there were no significant differences in the levels of CRP and ESR among different miR-146a genotype groups of AS patients, nor were there differences among miR-499 polymorphism groups. The two miRNA polymorphisms had no association with the disease activity of AS.


Association between ankylosing spondylitis and the miR-146a and miR-499 polymorphisms.

Xu HY, Wang ZY, Chen JF, Wang TY, Wang LL, Tang LL, Lin XY, Zhang CW, Chen BC - PLoS ONE (2015)

Association between variants of miR-146a (rs2910164) and miR-499 (rs3746444) and activity parameters.A. The levels of CRP among different miR-146a genotypes. No significant differences were found (CC vs. GG, P = 0.43; CC vs. GC, P = 0.79; GG vs. GC, P = 0.515). B. The levels of ESR among miR-146a genotypes. There were no significant differences among the three genotypes (CC vs. GG, P = 0.222; CC vs. GC, P = 0.249; GG vs. GC, P = 0.782). C. The levels of CRP among different miR-499 genotypes. No significant differences were found (TT vs. TC, P = 0.607). D. The levels of ESR among miR-499 genotypes. There were no significant differences (TT vs. TC, P = 0.759).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383612&req=5

pone.0122055.g001: Association between variants of miR-146a (rs2910164) and miR-499 (rs3746444) and activity parameters.A. The levels of CRP among different miR-146a genotypes. No significant differences were found (CC vs. GG, P = 0.43; CC vs. GC, P = 0.79; GG vs. GC, P = 0.515). B. The levels of ESR among miR-146a genotypes. There were no significant differences among the three genotypes (CC vs. GG, P = 0.222; CC vs. GC, P = 0.249; GG vs. GC, P = 0.782). C. The levels of CRP among different miR-499 genotypes. No significant differences were found (TT vs. TC, P = 0.607). D. The levels of ESR among miR-499 genotypes. There were no significant differences (TT vs. TC, P = 0.759).
Mentions: As shown in Fig. 1, there were no significant differences in the levels of CRP and ESR among different miR-146a genotype groups of AS patients, nor were there differences among miR-499 polymorphism groups. The two miRNA polymorphisms had no association with the disease activity of AS.

Bottom Line: A significant difference was observed in the miR-146a rs2910164G>C SNP.However, no significant association was found between the miR-499 rs3746444T>C variant and susceptibility to AS.Further studies are needed to validate our findings in a larger series, as well as in other ethnic backgrounds.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, China.

ABSTRACT
miRNAs are small, non-coding RNAs that regulate the expression of multiple target genes at the post-transcriptional level. Single-nucleotide polymorphisms (SNPs) in miRNA sequences may alter miRNA expression and have been implicated in the pathogenesis of multiple forms of arthritis, including rheumatoid arthritis (RA) and osteoarthritis. The present study explored the association between ankylosing spondylitis (AS) and two single nucleotide polymorphisms (SNPs), miR-146a rs2910164G>C and miR-499 rs3746444T>C, in a Han Chinese population. A case-control study consisting of 102 subjects with AS and 105 healthy controls was designed. The two miRNA SNPs were identified by direct sequencing. Subsequently, their gene and genotype frequencies were compared with healthy controls. A significant difference was observed in the miR-146a rs2910164G>C SNP. The frequency of the G allele was markedly higher in the AS patients than in the healthy controls (P = 0.005, Pc = 0.01, OR = 1.787), and the frequency of the GG genotype was higher in AS patients than in controls (P = 0.014, Pc = 0.042, OR = 2.516). However, no significant association was found between the miR-499 rs3746444T>C variant and susceptibility to AS. This is the first study to address the association between the miR-146a rs2910164G>C and miR-499 rs3746444T>C polymorphisms and AS, and it suggests a potential pathogenic factor for AS. Further studies are needed to validate our findings in a larger series, as well as in other ethnic backgrounds.

No MeSH data available.


Related in: MedlinePlus