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Inflammatory response to nano- and microstructured hydroxyapatite.

Mestres G, Espanol M, Xia W, Persson C, Ginebra MP, Ott MK - PLoS ONE (2015)

Bottom Line: Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated.Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations.However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

View Article: PubMed Central - PubMed

Affiliation: Materials in Medicine, Div. of Applied Materials Science, Dpt. Engineering Sciences, Uppsala University, Uppsala, Sweden.

ABSTRACT
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate's microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

No MeSH data available.


Related in: MedlinePlus

Release of Reactive Oxygen Species.Luminol-amplified luminescence assay (data normalized to relative units); b) Quantification of cell number after 1h, using LDH detection of lysed cells; c) Overlapping images of live/dead staining after 1h (n = 3).
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pone.0120381.g009: Release of Reactive Oxygen Species.Luminol-amplified luminescence assay (data normalized to relative units); b) Quantification of cell number after 1h, using LDH detection of lysed cells; c) Overlapping images of live/dead staining after 1h (n = 3).

Mentions: A luminol-amplified luminescence assay was performed to evaluate the release of ROS from cells in contact with different HA substrates. ROS reacts with luminol and emits light, which can be kinetically monitored. The luminescence emitted is shown as relative light units obtained after normalization to the highest luminescence signal (Fig. 9A). Cells on TCPS produced ROS only when activated with PMA. This activation is shown by an intense and broad luminescence peak with a maximum around 25 min. A very weak signal was detected for non-activated cells, indicating absence or very low quantity of ROS. Interestingly, when non-activated cells were added to the topographically different substrates, unique luminescence patterns were observed for each sample type. A strong signal was only detected for cells in contact with C-HA, which was attributed to their release of ROS, whereas cells on F-HA produced a very weak signal, similar to that of non-activated cells on TCPS.


Inflammatory response to nano- and microstructured hydroxyapatite.

Mestres G, Espanol M, Xia W, Persson C, Ginebra MP, Ott MK - PLoS ONE (2015)

Release of Reactive Oxygen Species.Luminol-amplified luminescence assay (data normalized to relative units); b) Quantification of cell number after 1h, using LDH detection of lysed cells; c) Overlapping images of live/dead staining after 1h (n = 3).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383585&req=5

pone.0120381.g009: Release of Reactive Oxygen Species.Luminol-amplified luminescence assay (data normalized to relative units); b) Quantification of cell number after 1h, using LDH detection of lysed cells; c) Overlapping images of live/dead staining after 1h (n = 3).
Mentions: A luminol-amplified luminescence assay was performed to evaluate the release of ROS from cells in contact with different HA substrates. ROS reacts with luminol and emits light, which can be kinetically monitored. The luminescence emitted is shown as relative light units obtained after normalization to the highest luminescence signal (Fig. 9A). Cells on TCPS produced ROS only when activated with PMA. This activation is shown by an intense and broad luminescence peak with a maximum around 25 min. A very weak signal was detected for non-activated cells, indicating absence or very low quantity of ROS. Interestingly, when non-activated cells were added to the topographically different substrates, unique luminescence patterns were observed for each sample type. A strong signal was only detected for cells in contact with C-HA, which was attributed to their release of ROS, whereas cells on F-HA produced a very weak signal, similar to that of non-activated cells on TCPS.

Bottom Line: Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated.Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations.However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

View Article: PubMed Central - PubMed

Affiliation: Materials in Medicine, Div. of Applied Materials Science, Dpt. Engineering Sciences, Uppsala University, Uppsala, Sweden.

ABSTRACT
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate's microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

No MeSH data available.


Related in: MedlinePlus