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Inflammatory response to nano- and microstructured hydroxyapatite.

Mestres G, Espanol M, Xia W, Persson C, Ginebra MP, Ott MK - PLoS ONE (2015)

Bottom Line: Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated.Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations.However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

View Article: PubMed Central - PubMed

Affiliation: Materials in Medicine, Div. of Applied Materials Science, Dpt. Engineering Sciences, Uppsala University, Uppsala, Sweden.

ABSTRACT
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate's microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

No MeSH data available.


Related in: MedlinePlus

Indirect contact assay supplementing extract with Ca and/or FBS.Proliferation of Raw 264.7 cells (6.5·103 cells per well) in C-HA and F-HA extracts supplemented with Ca and/or FBS. Four different extracts were used for each substrate. HA discs were soaked with complete media (cM, media supplemented with 10% FBS and 1% penicillin/streptomycin) that was either used after substrates soaking (cM) or was further supplemented with Ca (cM + Ca). Some other discs were soaked in media (M, containing no FBS) that was further supplemented with FBS (M + FBS) or supplemented with both FBS and Ca (M + FBS + Ca) (n = 4).
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pone.0120381.g008: Indirect contact assay supplementing extract with Ca and/or FBS.Proliferation of Raw 264.7 cells (6.5·103 cells per well) in C-HA and F-HA extracts supplemented with Ca and/or FBS. Four different extracts were used for each substrate. HA discs were soaked with complete media (cM, media supplemented with 10% FBS and 1% penicillin/streptomycin) that was either used after substrates soaking (cM) or was further supplemented with Ca (cM + Ca). Some other discs were soaked in media (M, containing no FBS) that was further supplemented with FBS (M + FBS) or supplemented with both FBS and Ca (M + FBS + Ca) (n = 4).

Mentions: Fig. 8 shows the results of cell proliferation using fresh medium (control), HA extracts and HA extracts supplemented with Ca, FBS or both Ca and FBS. Interestingly, the addition of Ca and FBS enhanced cell proliferation for both C-HA and F-HA extracts, although significantly more for C-HA extracts. At day 7, cell proliferation was mostly enhanced by the addition of both Ca and FBS; and the addition of only FBS enhanced cell proliferation more than addition of only Ca. At all time points, fresh medium had significantly higher cell numbers than any other condition.


Inflammatory response to nano- and microstructured hydroxyapatite.

Mestres G, Espanol M, Xia W, Persson C, Ginebra MP, Ott MK - PLoS ONE (2015)

Indirect contact assay supplementing extract with Ca and/or FBS.Proliferation of Raw 264.7 cells (6.5·103 cells per well) in C-HA and F-HA extracts supplemented with Ca and/or FBS. Four different extracts were used for each substrate. HA discs were soaked with complete media (cM, media supplemented with 10% FBS and 1% penicillin/streptomycin) that was either used after substrates soaking (cM) or was further supplemented with Ca (cM + Ca). Some other discs were soaked in media (M, containing no FBS) that was further supplemented with FBS (M + FBS) or supplemented with both FBS and Ca (M + FBS + Ca) (n = 4).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383585&req=5

pone.0120381.g008: Indirect contact assay supplementing extract with Ca and/or FBS.Proliferation of Raw 264.7 cells (6.5·103 cells per well) in C-HA and F-HA extracts supplemented with Ca and/or FBS. Four different extracts were used for each substrate. HA discs were soaked with complete media (cM, media supplemented with 10% FBS and 1% penicillin/streptomycin) that was either used after substrates soaking (cM) or was further supplemented with Ca (cM + Ca). Some other discs were soaked in media (M, containing no FBS) that was further supplemented with FBS (M + FBS) or supplemented with both FBS and Ca (M + FBS + Ca) (n = 4).
Mentions: Fig. 8 shows the results of cell proliferation using fresh medium (control), HA extracts and HA extracts supplemented with Ca, FBS or both Ca and FBS. Interestingly, the addition of Ca and FBS enhanced cell proliferation for both C-HA and F-HA extracts, although significantly more for C-HA extracts. At day 7, cell proliferation was mostly enhanced by the addition of both Ca and FBS; and the addition of only FBS enhanced cell proliferation more than addition of only Ca. At all time points, fresh medium had significantly higher cell numbers than any other condition.

Bottom Line: Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated.Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations.However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

View Article: PubMed Central - PubMed

Affiliation: Materials in Medicine, Div. of Applied Materials Science, Dpt. Engineering Sciences, Uppsala University, Uppsala, Sweden.

ABSTRACT
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate's microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

No MeSH data available.


Related in: MedlinePlus