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Inflammatory response to nano- and microstructured hydroxyapatite.

Mestres G, Espanol M, Xia W, Persson C, Ginebra MP, Ott MK - PLoS ONE (2015)

Bottom Line: Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated.Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations.However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

View Article: PubMed Central - PubMed

Affiliation: Materials in Medicine, Div. of Applied Materials Science, Dpt. Engineering Sciences, Uppsala University, Uppsala, Sweden.

ABSTRACT
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate's microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

No MeSH data available.


Related in: MedlinePlus

Indirect contact assay.Proliferation of Raw 264.7 cells in C-HA and F-HA extracts and in fresh media (indirect contact assay). 2·104 cells were seeded to each well. Cell number was quantified by LDH released after cell lysis (n = 4). * indicates p < 0.05.
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pone.0120381.g006: Indirect contact assay.Proliferation of Raw 264.7 cells in C-HA and F-HA extracts and in fresh media (indirect contact assay). 2·104 cells were seeded to each well. Cell number was quantified by LDH released after cell lysis (n = 4). * indicates p < 0.05.

Mentions: Fig. 6 shows the effect of cell proliferation using HA extracts as the medium being supplied to the cells (indirect method). At day 3, cell proliferation was only evident in the control wells using fresh medium, not for the cells cultured with extracts. The number of cells in fresh medium remained constant between day 3 and 7, whereas for both HA extracts, a prominent increase was observed. At day 14, a similar number of cells were present in all samples after a decrease in cell number for wells with C-HA extract and fresh medium.


Inflammatory response to nano- and microstructured hydroxyapatite.

Mestres G, Espanol M, Xia W, Persson C, Ginebra MP, Ott MK - PLoS ONE (2015)

Indirect contact assay.Proliferation of Raw 264.7 cells in C-HA and F-HA extracts and in fresh media (indirect contact assay). 2·104 cells were seeded to each well. Cell number was quantified by LDH released after cell lysis (n = 4). * indicates p < 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4383585&req=5

pone.0120381.g006: Indirect contact assay.Proliferation of Raw 264.7 cells in C-HA and F-HA extracts and in fresh media (indirect contact assay). 2·104 cells were seeded to each well. Cell number was quantified by LDH released after cell lysis (n = 4). * indicates p < 0.05.
Mentions: Fig. 6 shows the effect of cell proliferation using HA extracts as the medium being supplied to the cells (indirect method). At day 3, cell proliferation was only evident in the control wells using fresh medium, not for the cells cultured with extracts. The number of cells in fresh medium remained constant between day 3 and 7, whereas for both HA extracts, a prominent increase was observed. At day 14, a similar number of cells were present in all samples after a decrease in cell number for wells with C-HA extract and fresh medium.

Bottom Line: Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated.Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations.However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

View Article: PubMed Central - PubMed

Affiliation: Materials in Medicine, Div. of Applied Materials Science, Dpt. Engineering Sciences, Uppsala University, Uppsala, Sweden.

ABSTRACT
The proliferation and activation of leukocytes upon contact with a biomaterial play a crucial role in the degree of inflammatory response, which may then determine the clinical failure or success of an implanted biomaterial. The aim of this study was to evaluate whether nano- and microstructured biomimetic hydroxyapatite substrates can influence the growth and activation of macrophage-like cells. Hydroxyapatite substrates with different crystal morphologies consisting of an entangled network of plate-like and needle-like crystals were evaluated. Macrophage proliferation was evaluated on the material surface (direct contact) and also in extracts i.e. media modified by the material (indirect contact). Additionally, the effect of supplementing the extracts with calcium ions and/or proteins was investigated. Macrophage activation on the substrates was evaluated by quantifying the release of reactive oxygen species and by morphological observations. The results showed that differences in the substrate's microstructure play a major role in the activation of macrophages as there was a higher release of reactive oxygen species after culturing the macrophages on plate-like crystals substrates compared to the almost non-existent release on needle-like substrates. However, the difference in macrophage proliferation was ascribed to different ionic exchanges and protein adsorption/retention from the substrates rather than to the texture of materials.

No MeSH data available.


Related in: MedlinePlus